脑梗死患者循环circRNA表达谱的分析
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摘要
目的采用高通量芯片检测技术研究脑梗死患者循环circ RNA的差异表达谱,分析其可能的病理机制和临床相关情况。方法选取40例脑梗死患者为脑梗死组,入院后抽取血标本,另设40例健康者作对照(健康组)。两组均运用芯片检测循环circ RNA表达谱,进行组间比较,并运用生物信息学技术探讨其功能机制,同时对显著差异表达的前10个circ RNA建立生物信息分析。结果和健康组比较,脑梗死患者外周血中表达上调和下调的circ RNA有348个(P<0.01或0.05),其中上调186个,下调162个。通过生物信息网络资源检索发现,上、下调的前10位circ RNA均储存于中枢及外周等多个部位。结论脑梗死患者外周血中circ RNA的表达异于健康者,提示circ RNA可能参与调控脑梗死的发生、发展以及修复等过程。
Objective To explore the differential expression profile of circular RNA(circ RNA) in patients with cerebral infarction by using high-throughput chip detection method and analyze the possible pathological mechanism and clinical related conditions. 40 patients with cerebral infarction were selected as Cerebral Infarction Group, and their blood Methodssamples were taken after admission. Another 40 healthy subjects were set as Healthy Group and blood samples were taken as control. The microarray was used to detect the expression profile of circ RNA in both groups. Comparison between two groups was conducted, and the functional mechanism was discussed by using bioinformatics technology. Bioinformatics analysis was established for the first 10 circ RNAs with significant differential expression. Results Compared with the Healthy Group, the Cerebral Infarction Group had 348 up-regulated and down-regulated circ RNAs in peripheral blood(P<0.01, P<0.05), of which there were 186 up-regulated circ RNAs and 162 down-regulated circ RNAs. Through the retrieval of biological information network resources, the first 10 up-regulated and down-regulated circ RNAs were all stored in central nerve system and peripheral region. Conclusion The patients with cerebral infarction have the circ RNA expression profile in peripheral blood different from that of healthy people, suggesting that circ RNA may be involved in controlling the occurrence, development repair process of cerebral infarction.
Objective To explore the differential expression profile of circular RNA(circ RNA) in patients with cerebral infarction by using high-throughput chip detection method and analyze the possible pathological mechanism and clinical related conditions. 40 patients with cerebral infarction were selected as Cerebral Infarction Group, and their blood Methodssamples were taken after admission. Another 40 healthy subjects were set as Healthy Group and blood samples were taken as control. The microarray was used to detect the expression profile of circ RNA in both groups. Comparison between two groups was conducted, and the functional mechanism was discussed by using bioinformatics technology. Bioinformatics analysis was established for the first 10 circ RNAs with significant differential expression. Results Compared with the Healthy Group, the Cerebral Infarction Group had 348 up-regulated and down-regulated circ RNAs in peripheral blood(P<0.01, P<0.05), of which there were 186 up-regulated circ RNAs and 162 down-regulated circ RNAs. Through the retrieval of biological information network resources, the first 10 up-regulated and down-regulated circ RNAs were all stored in central nerve system and peripheral region. Conclusion The patients with cerebral infarction have the circ RNA expression profile in peripheral blood different from that of healthy people, suggesting that circ RNA may be involved in controlling the occurrence, development repair process of cerebral infarction.
引文
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[4]Rybak-wolf A,Stottmeister C,Gla?ar P,et al.Circular RNAs in the mammalian brain are highly abundant,conserved,and dynamically expressed[J].Molecular Cell,2015,58(5):870-885.
[5]Memczak S,Jens M,Elefsinioti A,et al.Circular RNAs are a large class of animal RNAs with regulatory potency[J].Nature,2013,495(21):333-338.
[6]吴江,贾建平,崔丽英,等.神经病学[M].北京:人民卫生出版社,2005:158-170.
[7]Lasda E,Parker R.Circular RNAs diversity of form and function[J].RNA,2014,20(12):1829-1842.
[8]Zhang X O,Wang H B,Zhang Y,et al.Complementary sequence-mediated exon circularization[J].Cell,2014,159(25):134-147.
[9]Umekage S,Kikuchi Y.In vivo circular RNA expression by the permuted intron-exon method[J].J Biosci Bioeng,2009,108(4):354-356.
[10]夏天,蒋孝明,陈晓敏,等.环状RNA:竞争性内源RNA新成员[J].中国细胞生物学学报,2013,35(10):1695-1700.
[11]You X,Vlatkovic I,Babic A,et al.Neural circular RNAs are derived from synaptic genes and regulated by development and plasticity[J].Nat Neurosci,2015,18(4):603-610.