摘要
目的研究黔产细锥香茶菜Isodon coetsa的化学成分。方法采用硅胶、凝胶柱色谱、制备型HPLC等方法进行分离纯化,通过理化性质、光谱数据结合参考文献鉴定化合物结构。结果从细锥香茶菜95%甲醇提取物中分离得到了19个化合物,分别鉴定为β-谷甾醇(1)、豆甾醇(2)、β-胡萝卜苷(3)、亚麻酸甲酯(4)、油酸-1,3-甘油二酯(5)、熊果酸(6)、齐墩果酸(7)、3β-羟基-乌索-20-烯-28-酸(8)、白桦脂酸(9)、苦参黄素(10)、山楂酸(11)、2α羟基-熊果酸(12)、2α,3α-二羟基-12-烯-28-乌苏酸(13)、2α,3α,24-三羟基-12-烯-28-齐墩果酸(14)、2α,3α,24-三羟基-12-烯-28-熊果酸(15)、芒柄花素(16)、大黄素(17)、尿嘧啶(18)、蔗糖(19)。结论化合物2、4、5、9、11、13~19均为首次从该植物中分离得到,化合物11、17、19为首次从香茶菜属植物中分离得到。
Objective To investigate chemical constituents from Isodon coetsa distributed in Guizhou. Methods Chemical constituents were isolated and purified by chromatography with silica gel, Sephadex LH-20, and semi-preparative HPLC, and their structures were identified by analysis of spectroscopic evidences and physicochemical properties as well as relevant references. Results A total of 19 compounds were isolated from plant material extracted with 95% aqueous methanol, which were elucidated as β-sitosterol(1), stigmasterol(2), β-daucosterol(3), methyl linolenat(4), 9,12-octadecadienoic acid-2-hydroxy-1,3-propanedinyl ester(5), ursolic acid(6), oleanoic acid(7), 3β-hydroxy-urs-20-en-28-oic acid(8), betulinic acid(9), kurarinone(10), maslinic acid(11), 2α-hydroxy ursolic acid(12), 2α,3α-dihydroxy-urs-12-en-28-oic acid(13), 2α,3α,24-trihydrxyolean-12-en-28-oic acid(14), 2α,3β,24-trihydroxyolean-12-en-28-oic acid(15), formononetin(16), emodin(17), uracil(18), and sucrose(19). Conclusion Compounds 2, 4, 5, 9, 11, 13—19 are isolated from this plant for first time, and compounds 11, 17, 19 are isolated from the genus of Isodon for the first time.
引文
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