2个荔枝品种体胚发生过程中几种抗氧化酶与多胺氧化酶活性的变化
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摘要
以‘新球蜜荔’和‘大丁香’2个荔枝品种胚性愈伤组织为材料,研究了‘新球蜜荔’(XQTD)和‘大丁香’(DDXTD)接种在TD(MS+0.1 mg/L NAA+0.5 mg/L TDZ+0.1 g/L肌醇+0.4 g/L水解乳蛋白(LH)+100 mL/L椰汁+60g/L蔗糖+10 g/L琼脂)培养基与‘新球蜜荔’接种在TX(MS+0.1 mg/L NAA+5 mg/L ZT+0.1 g/L肌醇+0.4 g/L水解乳蛋白(LH)+100 ml/L椰汁+60 g/L蔗糖+10 g/L琼脂)培养基(XQTX)上各培养物体胚发生过程中抗氧化酶与多胺氧化酶活性变化。结果表明:体胚发生过程中,三者的可溶性蛋白含量变化都呈单峰曲线,峰值出现在第6天,在前12 d的培养中,XQTD高于XQTX,仅在第9天,DDXTD高于XQTD。三者的丙二醛(MDA)含量和超氧化物歧化酶(SOD)活性都变化平缓且差异不大。三者的过氧化物酶(POD)活性变化都呈单峰曲线,XQTD的峰值出现在第9天,其余出现在第12天,体胚发生过程中DDXTD最高。XQTD和XQTX的过氧化氢酶(CAT)活性都呈先降后升趋势,而DDXTD只是后期下降。三者的多胺氧化酶(PAO)活性变化都呈先降后升趋势,在前15 d的培养中,XQTX和DDXTD都低于XQTD,且DDXTD在前6 d里基本无变化。培养物中可溶性蛋白含量、POD和PAO活性变化可能与‘新球蜜荔’及‘大丁香’的体胚发生效率差异有关。
Changes of several antioxidant enzymes and polyamine oxidase activity in embryonic callus of litchi cultivars ‘Xinqiumili' and ‘Dadingxiang' were studied during the somatic embryogenesis induced on different induction media.The results showed that there was one peak curve of soluble protein content in both the embryonic callus of ‘Dadingxaing'(DDXTD) and ‘Xingqiumili'(XQTD)inoculated on TD medium(MS + 0.1 mg/L NAA + 0.5 mg/L TDZ + 0.1 g/L inositol + 0.4 g/L LH + 100 ml/L coconut juice + 60 g/L sucrose + 10 g/L agar), and the embryonic callus of ‘Xingqiumili'(XQTX)inoculated on TX medium(MS + 0.1 mg/L NAA + 5 mg/L ZT + 0.1 g/L inositol + 0.4 g/L LH + 100 ml/L coconut juice + 60 g/L sucrose + 10 g/L agar), with the peak occurring on the 6 th day after inoculation(th day). The content of soluble protein in XQTD was higher than that in XQTX from 6 th to 12 th day, while DDXTD had higher soluble protein content on 9 th day than XQTD. The malondialdehyde(MDA) content and the superoxide dismutase(SOD) activity in XQTX, XQTD and DDXTD had gentle variations and little differences. During the process of somatic embryogenesis, the changes of peroxidase(POD) activity in XQTX, XQTD and DDXTD showed a single peak curve, with the peak of XQTD appeared on the 9 th day, the rest appeared on the 12 th day. The highest POD activity was in DDXTD, and the lowest was on XQTD. The catalase(CAT) activity in XQTD and XQTX increased after decrease during somatic embryogenesis, while the catalase(CAT) activity in DDXTD only decreased in the later period. The polyamine oxidase(PAO) activity in XQTD, XQTX and DDXTD all increased after a slight decrease in the early 6 days. PAO activity in XQTD was higher both than that in XQTX from the 6 th to 15 th day, and than that in DDXTD from the 0 to 15 th day. The soluble protein content, POD activity and PAO activity may be related to the different frequency of the somatic embryogenesis of 'Xinqiumili' and 'Dadingxiang'.
Changes of several antioxidant enzymes and polyamine oxidase activity in embryonic callus of litchi cultivars ‘Xinqiumili' and ‘Dadingxiang' were studied during the somatic embryogenesis induced on different induction media.The results showed that there was one peak curve of soluble protein content in both the embryonic callus of ‘Dadingxaing'(DDXTD) and ‘Xingqiumili'(XQTD)inoculated on TD medium(MS + 0.1 mg/L NAA + 0.5 mg/L TDZ + 0.1 g/L inositol + 0.4 g/L LH + 100 ml/L coconut juice + 60 g/L sucrose + 10 g/L agar), and the embryonic callus of ‘Xingqiumili'(XQTX)inoculated on TX medium(MS + 0.1 mg/L NAA + 5 mg/L ZT + 0.1 g/L inositol + 0.4 g/L LH + 100 ml/L coconut juice + 60 g/L sucrose + 10 g/L agar), with the peak occurring on the 6 th day after inoculation(th day). The content of soluble protein in XQTD was higher than that in XQTX from 6 th to 12 th day, while DDXTD had higher soluble protein content on 9 th day than XQTD. The malondialdehyde(MDA) content and the superoxide dismutase(SOD) activity in XQTX, XQTD and DDXTD had gentle variations and little differences. During the process of somatic embryogenesis, the changes of peroxidase(POD) activity in XQTX, XQTD and DDXTD showed a single peak curve, with the peak of XQTD appeared on the 9 th day, the rest appeared on the 12 th day. The highest POD activity was in DDXTD, and the lowest was on XQTD. The catalase(CAT) activity in XQTD and XQTX increased after decrease during somatic embryogenesis, while the catalase(CAT) activity in DDXTD only decreased in the later period. The polyamine oxidase(PAO) activity in XQTD, XQTX and DDXTD all increased after a slight decrease in the early 6 days. PAO activity in XQTD was higher both than that in XQTX from the 6 th to 15 th day, and than that in DDXTD from the 0 to 15 th day. The soluble protein content, POD activity and PAO activity may be related to the different frequency of the somatic embryogenesis of 'Xinqiumili' and 'Dadingxiang'.
引文
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[4]刘宝宝,张士璀.多胺氧化酶在多胺代谢中的作用[J].鲁东大学学报(自然科学版),2014,30(4):336-341.
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[9]金玉佩,刘佳,纪若璇,等.楸树体胚发生过程中5种酶的活性变化研究[J].热带作物学报,2017,38(2):252-257.
[10]关正君,郭斌,尉亚辉.樱桃番茄叶体细胞胚发生过程中抗氧化酶活性和生理参数的变化[J].核农学报,2011,25(3):594-601.
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[14]Smith T A.Purification and properties of the polyamine oxidase of barley plants[J].Phytochemistry,1972,11(3):899-910.
[15]陈伟.荔枝(Litchi chinensis Sonn.)胚胎发育的生理生化基础[D].福州:福建农林大学,2000.
[16]郭绍云.荔枝、龙眼离体培养及再生植株的研究[D].南宁:广西大学,2014.
[17]佘茂云,殷桂香,杜丽璞,等.非生物胁迫处理对植物离体组织培养再生效果影响研究进展[J].科技导报,2014,32(2):97-103.
[18]詹园凤,吴震,金潇潇,等.大蒜体细胞胚胎发生过程中抗氧化酶活性变化及某些生理特征[J].西北植物学报,2006,26(9):1 799-1 802.
[19]邢更妹,李杉,崔凯荣,等.植物体细胞胚发生中某些机理探讨[J].自然科学进展,2000,10(8):14-22.
[20]王义,赵文君,孙春玉,等.人参体细胞胚胎发生过程中的生理变化[J].东北师大学报(自然科学版),2008,40(2):93-97.
[21]崔凯荣,任红旭,邢更妹,等.枸杞组织培养中抗氧化酶活性与体细胞胚发生相关性的研究[J].兰州大学学报,1998,34(3):93-99.
[22]陈伟,吕柳.荔枝胚珠中多胺含量变化与胚胎发育的关系[J].热带亚热带植物学报,2000,8(3):229-234.
[2]邝哲师,周丽侬,马雪筠,等.荔枝体胚发生及成苗研究[J].广东农业科学,1997,24(1):16-18.
[3]王果,李焕苓,王家保.2个海南荔枝稀优品种花药培养诱导胚性愈伤组织的研究[J].热带作物学报,2013,34(4):669-674.
[4]刘宝宝,张士璀.多胺氧化酶在多胺代谢中的作用[J].鲁东大学学报(自然科学版),2014,30(4):336-341.
[5]辛福梅.栓皮栎体胚发生生理生化特性的研究[D].西安:西北农林科技大学,2007.
[6]张慧君,华玉伟,黄天带,等.橡胶树体胚发生过程中的生理生化特性[J].热带农业科学,2014,34(10):12-14,18.
[7]邓白平,陈小飞,鲁旭东,等.石刁柏体细胞胚发生过程中多胺代谢酶和过氧化氢清除酶的活性变化[J].植物生理学通讯,2008(4):647-650.
[8]黄浅.荔枝TPEC高频率体胚发生及荔枝与龙眼原生质体融合研究[D].福州:福建农林大学,2008.
[9]金玉佩,刘佳,纪若璇,等.楸树体胚发生过程中5种酶的活性变化研究[J].热带作物学报,2017,38(2):252-257.
[10]关正君,郭斌,尉亚辉.樱桃番茄叶体细胞胚发生过程中抗氧化酶活性和生理参数的变化[J].核农学报,2011,25(3):594-601.
[11]曹建康,姜微波,赵玉梅.果蔬采后生理生化实验指导[M].北京:中国轻工业出版社,2007.
[12]Nag S,Saha K,Choudhuri M A.A rapid and sensitive assay method for measuring amine oxidase based on hydrogen peroxide-titanium complex formation[J].Plant Science,2000,157(2):157-163.
[13]汪天,郭世荣,刘俊,等.多胺氧化酶检测方法的改进及其在低氧水培黄瓜根系中的应用[J].植物生理学通讯,2004(3):358-360.
[14]Smith T A.Purification and properties of the polyamine oxidase of barley plants[J].Phytochemistry,1972,11(3):899-910.
[15]陈伟.荔枝(Litchi chinensis Sonn.)胚胎发育的生理生化基础[D].福州:福建农林大学,2000.
[16]郭绍云.荔枝、龙眼离体培养及再生植株的研究[D].南宁:广西大学,2014.
[17]佘茂云,殷桂香,杜丽璞,等.非生物胁迫处理对植物离体组织培养再生效果影响研究进展[J].科技导报,2014,32(2):97-103.
[18]詹园凤,吴震,金潇潇,等.大蒜体细胞胚胎发生过程中抗氧化酶活性变化及某些生理特征[J].西北植物学报,2006,26(9):1 799-1 802.
[19]邢更妹,李杉,崔凯荣,等.植物体细胞胚发生中某些机理探讨[J].自然科学进展,2000,10(8):14-22.
[20]王义,赵文君,孙春玉,等.人参体细胞胚胎发生过程中的生理变化[J].东北师大学报(自然科学版),2008,40(2):93-97.
[21]崔凯荣,任红旭,邢更妹,等.枸杞组织培养中抗氧化酶活性与体细胞胚发生相关性的研究[J].兰州大学学报,1998,34(3):93-99.
[22]陈伟,吕柳.荔枝胚珠中多胺含量变化与胚胎发育的关系[J].热带亚热带植物学报,2000,8(3):229-234.