龙门山地震带不同植被类型土壤微生物群落多样性分析
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摘要
采用BIOLOG-ECO (Ecology Plate,ECO)技术,探讨龙门山地震带——银厂沟地区杂木林、竹林和松树林3种植被类型对土壤微生物群落多样性的影响.结果表明,随着培养时间的延长,土壤微生物群落的平均颜色变化率(Average Well Color Development,AWCD)均呈"S"型曲线增长,但3种植被类型土壤的AWCD值在增长速率和最大值方面存在一定的差异性;碳水化合物类、氨基酸类、醇类和酯类碳源是土壤微生物群落主要利用的碳源类型;当培养0～144 h时,竹林微生物群落的多样性最高,其次是杂木林,松树林最低,当培养144～240 h时,杂木林微生物群落的多样性高于竹林,松树林最低.研究结果说明植被类型可以影响其土壤微生物群落的多样性,同时也为龙门山地震带土壤生态系统的恢复和重建提供了科学依据.
BIOLOG-ECO technology was used to explore the effect of three types of vegetation,including mixed forest,bamboo forest and pine forest on the diversity of soil microbial community in Longmen mountain seismic zone-Yinchanggou area. The results showed that the average color change rate(AWCD) of soil microbial communities was a "S"shape curve with the increase of incubation time. However,there is a certain difference in the growth rate and maximum value of the AWCD values of different types of vegetation soils microbial communities. The main carbon sources of soil microbial community utilization were carbohydrates,amino acids,alcohols and esters. The metabolic diversity of soil microbial community of bamboo forest was the greatest followed by the mixed forest,and the lowest was pine forest when the incubation time were 0 ～ 144 h. The microbial diversity of soil microbial community of the mixed forest was the highest followed by the bamboo forest,and the lowest was also in the pine forest when the incubation time were 144 ～ 240 h. Earthquakes undermined the surface vegetation and soil structure directly,meanwhile,affected the diversity of soil microbial community. The results of this study indicated that vegetation types could affect the diversity of soil microbial communities and provide scientific basis for the restoration and reconstruction of the soil ecosystem in the Longmen mountain seismic zone.
BIOLOG-ECO technology was used to explore the effect of three types of vegetation,including mixed forest,bamboo forest and pine forest on the diversity of soil microbial community in Longmen mountain seismic zone-Yinchanggou area. The results showed that the average color change rate(AWCD) of soil microbial communities was a "S"shape curve with the increase of incubation time. However,there is a certain difference in the growth rate and maximum value of the AWCD values of different types of vegetation soils microbial communities. The main carbon sources of soil microbial community utilization were carbohydrates,amino acids,alcohols and esters. The metabolic diversity of soil microbial community of bamboo forest was the greatest followed by the mixed forest,and the lowest was pine forest when the incubation time were 0 ～ 144 h. The microbial diversity of soil microbial community of the mixed forest was the highest followed by the bamboo forest,and the lowest was also in the pine forest when the incubation time were 144 ～ 240 h. Earthquakes undermined the surface vegetation and soil structure directly,meanwhile,affected the diversity of soil microbial community. The results of this study indicated that vegetation types could affect the diversity of soil microbial communities and provide scientific basis for the restoration and reconstruction of the soil ecosystem in the Longmen mountain seismic zone.
引文
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[6]吴等等,宋志文,徐爱玲,等.青岛市不同功能区冬季空气微生物群落代谢与多样性特征[J].生态学报,2015,35(7):2277-2284.
[7]姚晓东,王娓,曾辉.磷脂脂肪酸法在土壤微生物群落分析中的应用[J].微生物学通报,2016,43(9):2086-2095.
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[10]邱洁,徐丽丽,钱叶,等.不同桑品种的根际土壤细菌群落及土壤理化性质研究[J].蚕业科学,2017,43(4):26-34.
[11]陈喜蓉,金映虹,欧滨,等.基于BIOLOG-ECO技术分析不同林下种植竹荪地土壤微生物功能多样性[J].基因组学与应用生物学,2017,36(1):370-375.
[12]吴则焰,林文雄,陈志芳,等.武夷山国家自然保护区不同植被类型土壤微生物群落特征[J].应用生态学报,2013,24(8):2301-2309.
[13]张广帅,林勇明,马瑞丰,等.汶川地震灾区不同气候区土壤微生物群落碳源代谢多样性[J].环境科学,2015,36(2):669-677.
[14]向泽宇,张莉,张全发,等.青海不同林分类型土壤养分与微生物功能多样性[J].林业科学,2014,50(4):22-31.
[15]朱平,陈仁升,宋耀选,等.祁连山不同植被类型土壤微生物群落多样性差异[J].草业学报,2015,24(6):75-84.
[16]韩世忠,高人,李爱萍,等.中亚热带地区两种森林植被类型土壤微生物群落结构[J].应用生态学报,2015,26(7):2151-2158.
[17]张毅,熊春莲,蔡艳,等.震后土壤微生物分布状况研究[J].西南农业学报,2009,22(5):1404-1409.
[18]王蕾,曾森,周云帆.汶川震后植被恢复趋势与地形关系研究[J].长沙大学学报,2015,29(5):14-16.
[19]李晓琴.龙门山地区山地旅游资源特征及开发模式探讨[J].地理与地理信息科学,2010,26(2):107-110.
[20]刘守江,张斌,杨清伟,等.汶川地震非规范滑坡体上植被的自然恢复能力研究——以彭州银厂沟谢家店子滑坡体为例[J].山地学报,2010,28(3):373-378.
[21]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科学技术出版社,2000:12-193.
[22] MICHIEL Rutgers,MARJA Wouterse,SYTSKE M Drost,et al. Monitoring soil bacteria with community-level physiological profiles using BIOLOGTMECO-plates in the Netherlands and Europe[J]. Applied Soil Ecology,2016,97:23-35.
[23]席劲瑛,胡洪营,钱易,等. BIOLOG方法在环境微生物群落研究中的应用[J].微生物学报,2003,43(1):138-141.
[24] ZHANG Hai-fang,LI Gang,SONG Xiao-long,et al. Changes in soil microbial functional diversity under different vegetation restoration patterns for Hulunbeier Sandy Land[J]. Acta Ecologica Sinica,2013,33(1):38-44.
[25] ROBIN Duponnois,PAUGYA M,JANE Thioulouse,et al. Functional diversity of soil microbial community,rock phosphate dissolution and growth of Acacia seyal as influenced by grass-,litter-and soil-feeding termite nest structure amendments[J]. Geoderma,2005,124(3/4):349-361.
[26]张海涵,唐明,陈辉,等.黄土高原5种造林树种菌根根际土壤微生物群落多样性研究[J].北京林业大学学报,2008,30(3):85-90.
[27]欧阳志云,徐卫华,王学志,等.汶川大地震对生态系统的影响[J].生态学报,2008,28(12):5801-5809.
[28]赵丽丽,钟哲科,史作民.汶川地震对岷江柏林土壤微生物群落及养分的影响[J].土壤通报,2016,47(1):98-104.
[29]唐杰,徐青锐,王立明,等.若尔盖高原湿地不同退化阶段的土壤细菌群落多样性[J].微生物学通报,2011,38(5):677-686.
[30]钱叶,侯怡铃,邱洁,等.龙门山地震带土壤细菌多样性的研究[J].土壤通报,2017,48(5):1093-1101.
[31]王春香,田宝玉,吕睿瑞,等.西双版纳地区热带雨林土壤酸杆菌(Acidobacteria)群体结构和多样性分析[J].微生物学通报,2010,37(1):24-29.
[32] ROBERT Griffiths,BRUCE C Thomson,PHILLIP James,et al. The bacterial biogeography of British soils[J]. Environmental Micro Biology,2011,13(6):1642-1654.
[33]淡俊豪,齐绍武,黎娟,等.生石灰对酸性土壤p H值及微生物群落功能多样性的影响[J].西南农业学报,2017(12):2739-2745.
[34] TRAUTE Heidi Anderson,KLAUS Heinz Domsch. Soil microbial biomass:the ECO-physiological approach[J]. Soil Biology&Biochemistry,2010,42(12):2039-2043.
[35] LI Hui,YE Dan-dan,WANG Xu-gao,et al. Soil bacterial communities of different natural forest types in Northeast China[J]. Plant and Soil,2014,383(1):203-216.
[36] ZHOU Jiang,LI Ping,WANG Yan-hong,et al. Effects of roxarsone on the functional diversity of soil microbial community[J]. International Biodeterioration&Biodegradation,2013,76(1):32-35.
[2]郭金瑞,宋振伟,朱平,等.长期不同种植模式对东北黑土微生物群落结构与土壤理化性质的影响[J].土壤通报,2016,47(2):353-359.
[3]沈萍,陈向东.微生物学复兴的机遇挑战和趋势[J].微生物学报,2010,50(1):1-6.
[4]贾国梅,王刚,陈芳清,等.子午岭植被演替过程中土壤生物学特性的动态[J].生态环境,2007,16(5):140-143.
[5] LISBET Holm Bach,JOHN Arvid Grytnes,RUNE Halvorsen,et al. Tree influence on soil microbial community structure[J]. Soil Biology and Biochemistry,2010,42(11):1934-1943.
[6]吴等等,宋志文,徐爱玲,等.青岛市不同功能区冬季空气微生物群落代谢与多样性特征[J].生态学报,2015,35(7):2277-2284.
[7]姚晓东,王娓,曾辉.磷脂脂肪酸法在土壤微生物群落分析中的应用[J].微生物学通报,2016,43(9):2086-2095.
[8] VICKY Mckinley,AARON D Peacock,DAVID C White. Microbial community PLFA and PHB responses to Ecosystem restoration in tallgrass prairie soils[J]. Soil Biolgy&Biochemistry,2005,37(10):1946-1958.
[9] WANG Lan,ZHANG Jing,LI Huilin,et al. Shift in the microbial community composition of surface water and sediment along an urban river[J]. Science of the Total Environment,2018,627:600-612.
[10]邱洁,徐丽丽,钱叶,等.不同桑品种的根际土壤细菌群落及土壤理化性质研究[J].蚕业科学,2017,43(4):26-34.
[11]陈喜蓉,金映虹,欧滨,等.基于BIOLOG-ECO技术分析不同林下种植竹荪地土壤微生物功能多样性[J].基因组学与应用生物学,2017,36(1):370-375.
[12]吴则焰,林文雄,陈志芳,等.武夷山国家自然保护区不同植被类型土壤微生物群落特征[J].应用生态学报,2013,24(8):2301-2309.
[13]张广帅,林勇明,马瑞丰,等.汶川地震灾区不同气候区土壤微生物群落碳源代谢多样性[J].环境科学,2015,36(2):669-677.
[14]向泽宇,张莉,张全发,等.青海不同林分类型土壤养分与微生物功能多样性[J].林业科学,2014,50(4):22-31.
[15]朱平,陈仁升,宋耀选,等.祁连山不同植被类型土壤微生物群落多样性差异[J].草业学报,2015,24(6):75-84.
[16]韩世忠,高人,李爱萍,等.中亚热带地区两种森林植被类型土壤微生物群落结构[J].应用生态学报,2015,26(7):2151-2158.
[17]张毅,熊春莲,蔡艳,等.震后土壤微生物分布状况研究[J].西南农业学报,2009,22(5):1404-1409.
[18]王蕾,曾森,周云帆.汶川震后植被恢复趋势与地形关系研究[J].长沙大学学报,2015,29(5):14-16.
[19]李晓琴.龙门山地区山地旅游资源特征及开发模式探讨[J].地理与地理信息科学,2010,26(2):107-110.
[20]刘守江,张斌,杨清伟,等.汶川地震非规范滑坡体上植被的自然恢复能力研究——以彭州银厂沟谢家店子滑坡体为例[J].山地学报,2010,28(3):373-378.
[21]鲁如坤.土壤农业化学分析方法[M].北京:中国农业科学技术出版社,2000:12-193.
[22] MICHIEL Rutgers,MARJA Wouterse,SYTSKE M Drost,et al. Monitoring soil bacteria with community-level physiological profiles using BIOLOGTMECO-plates in the Netherlands and Europe[J]. Applied Soil Ecology,2016,97:23-35.
[23]席劲瑛,胡洪营,钱易,等. BIOLOG方法在环境微生物群落研究中的应用[J].微生物学报,2003,43(1):138-141.
[24] ZHANG Hai-fang,LI Gang,SONG Xiao-long,et al. Changes in soil microbial functional diversity under different vegetation restoration patterns for Hulunbeier Sandy Land[J]. Acta Ecologica Sinica,2013,33(1):38-44.
[25] ROBIN Duponnois,PAUGYA M,JANE Thioulouse,et al. Functional diversity of soil microbial community,rock phosphate dissolution and growth of Acacia seyal as influenced by grass-,litter-and soil-feeding termite nest structure amendments[J]. Geoderma,2005,124(3/4):349-361.
[26]张海涵,唐明,陈辉,等.黄土高原5种造林树种菌根根际土壤微生物群落多样性研究[J].北京林业大学学报,2008,30(3):85-90.
[27]欧阳志云,徐卫华,王学志,等.汶川大地震对生态系统的影响[J].生态学报,2008,28(12):5801-5809.
[28]赵丽丽,钟哲科,史作民.汶川地震对岷江柏林土壤微生物群落及养分的影响[J].土壤通报,2016,47(1):98-104.
[29]唐杰,徐青锐,王立明,等.若尔盖高原湿地不同退化阶段的土壤细菌群落多样性[J].微生物学通报,2011,38(5):677-686.
[30]钱叶,侯怡铃,邱洁,等.龙门山地震带土壤细菌多样性的研究[J].土壤通报,2017,48(5):1093-1101.
[31]王春香,田宝玉,吕睿瑞,等.西双版纳地区热带雨林土壤酸杆菌(Acidobacteria)群体结构和多样性分析[J].微生物学通报,2010,37(1):24-29.
[32] ROBERT Griffiths,BRUCE C Thomson,PHILLIP James,et al. The bacterial biogeography of British soils[J]. Environmental Micro Biology,2011,13(6):1642-1654.
[33]淡俊豪,齐绍武,黎娟,等.生石灰对酸性土壤p H值及微生物群落功能多样性的影响[J].西南农业学报,2017(12):2739-2745.
[34] TRAUTE Heidi Anderson,KLAUS Heinz Domsch. Soil microbial biomass:the ECO-physiological approach[J]. Soil Biology&Biochemistry,2010,42(12):2039-2043.
[35] LI Hui,YE Dan-dan,WANG Xu-gao,et al. Soil bacterial communities of different natural forest types in Northeast China[J]. Plant and Soil,2014,383(1):203-216.
[36] ZHOU Jiang,LI Ping,WANG Yan-hong,et al. Effects of roxarsone on the functional diversity of soil microbial community[J]. International Biodeterioration&Biodegradation,2013,76(1):32-35.