酸荔枝核提取物对α-葡萄糖苷酶的抑制作用
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摘要
探讨酸荔枝提取物对α-葡萄糖苷酶的抑制活性。将酸荔枝干燥核粉碎,用乙醇超声波法提取,对提取物用石油醚、乙酸乙酯、正丁醇进行萃取。采用α-葡萄糖苷酶体外活性抑制模型,测定酸荔枝的4种提取物对α-葡萄糖苷酶的抑制活性。结果表明,这4种提取物对α-葡萄苷酶活性有较强的抑制作用,抑制活性均高于阿卡波糖,其中水部位的抑制作用最强,提示酸荔枝在抗糖尿病产品开发方面具有很好的应用前景。试验揭示酸荔枝核提取物对α-葡萄糖苷酶具有一定抑制作用。
The inhibitory activity of acid litchi extract of lychee to α-glucosidase was investigated. The dried acid litchi seed pulverized, and extracted by ethanol ultrasonic method, the extract with petroleum ether, ethyl acetate, n-butanolextraction to purify. Using α-glucosidase inhibitory activity in vitro model, the inhibitory activity of four acid litchi extracts to α-glucosidase was determined. The results showed that α-glucosidase activity was strongly inhibited by four extracts, and the inhibitory activity was higher than acarbose. The part of water had strongest inhibition, suggesting that acid litchi had prospects of development of anti-diabetic litchi product. The acid litchi seed extract had inhibitory activity on α-glucosidase to a certain extent.
The inhibitory activity of acid litchi extract of lychee to α-glucosidase was investigated. The dried acid litchi seed pulverized, and extracted by ethanol ultrasonic method, the extract with petroleum ether, ethyl acetate, n-butanolextraction to purify. Using α-glucosidase inhibitory activity in vitro model, the inhibitory activity of four acid litchi extracts to α-glucosidase was determined. The results showed that α-glucosidase activity was strongly inhibited by four extracts, and the inhibitory activity was higher than acarbose. The part of water had strongest inhibition, suggesting that acid litchi had prospects of development of anti-diabetic litchi product. The acid litchi seed extract had inhibitory activity on α-glucosidase to a certain extent.
引文
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[13]LI T,ZHANG X D,SONG Y W,et al.Amicroplate-based screening method for alpha-glucosidase inhibitors[J].Chin JClinpharmacol Ther,2005,10(10):1129.
[14]张丽,李彩芳李晓梅,等,加拿大蓬α-葡萄糖苷酶抑制作用[J].河南大学学报(医学版),2008,27(4):39-41.
[15]康文艺,张丽,张倩.黄连提取物对α-葡萄糖苷酶抑制作用研究[J].天然产物研究与开发,2009,21:992-994,1014.
[2]蔡长河,曾庆孝,段冬洋.荔枝的营养保健功能、加工研究现状及开发利用前景[J].食品科学,2005,26(8):530-533.
[3]陆志科,黎深.荔枝核活性成分分析及其提取物抗氧化性能研究[J].食品科学,2009,30(23):110-113.
[4]冯卫华,于立梅,秦艳,等.荔枝多酚的抗氧化性动力学及稳定性[J].食品科学,2011,23(15):5-9.
[5]姚伙旺,曾秋平,劳永民,等.食品添加剂对荔枝果皮花色素苷稳定性影响的研究[J].食品科学,2006,27(5):152-156.
[6]姜振国.荔枝核的化学成分及降血糖活性研究[D].长春长春中医药大学,2011.
[7]郭洁文,潘竞锵.荔枝和荔枝核的化学成分、生物活性及药理作用研究[J].中国新药杂志,2006,15(8):585-588.
[8]谢纹,沈洁,赵晓山.糖尿病的中西医治疗近况[J].中国临床康复,2003,7(21):2973.
[9]杨燕军,梁北梅.荔枝核抗糖尿病有效部位总皂苷的含量测定[J].广东药学,2004,14(6):13-15.
[10]潘竞锵,郭洁文,韩超.荔枝核的药理实验研究[J].中国新药杂志,2009,9(1):14-16.
[11]邓志军,郭洁文,潘竞锵.荔枝和荔枝核及其有效部位的药理及药效学作用[J].今日药学,2009,19(5):7-9.
[12]杨燕军,梁北梅.荔枝核抗糖尿病有效部位总皂苷的含量测定[J].广东药学,2004,14(6):13-15.
[13]LI T,ZHANG X D,SONG Y W,et al.Amicroplate-based screening method for alpha-glucosidase inhibitors[J].Chin JClinpharmacol Ther,2005,10(10):1129.
[14]张丽,李彩芳李晓梅,等,加拿大蓬α-葡萄糖苷酶抑制作用[J].河南大学学报(医学版),2008,27(4):39-41.
[15]康文艺,张丽,张倩.黄连提取物对α-葡萄糖苷酶抑制作用研究[J].天然产物研究与开发,2009,21:992-994,1014.