Box-Behnken响应面法优选地芍苣冬颗粒提取工艺
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摘要
目的:采用Box-Behnken响应面法优选地芍苣冬颗粒的提取工艺。方法:通过单因素试验考查浸泡时间、提取时间、加水倍数和提取次数对地芍苣冬颗粒中天麻素、芍药苷含量的影响。基于单因素的结果,应用BoxBehnken响应面法考察浸泡时间、提取时间、加水倍数3个因素对地芍苣冬颗粒中天麻素、芍药苷含量以及离体血管舒张率的影响,对结果进行二项式方程拟合,用响应面法预测最佳水提工艺。结果:二项式拟合方程预测性良好,理论预测值与实际值偏差较小。在最佳提取工艺条件(浸泡时间为1. 5 h,加10倍量水提取1 h,共提取2次)下,天麻素平均质量分数为0. 59 mg·g-1,芍药苷平均质量分数为6. 35 mg·g-1,平均离体血管舒张率为71. 03%。结论:此工艺稳定、方便,准确快速地优化地芍苣冬颗粒的提取工艺。
Objective: This paper aims to optimize the extraction process of Dishaojudong granule by using the Box-Behnken response surface method. Methods: The effects of immersion time,extraction time,extraction multiples and extraction times on the content of gastrotin,paeoniflorin were studied by single factor test. Based on the results of single factor,the effects of the study on the time of immersion,the time of extraction and the extraction multiples were studied. The effect of the three factors on the content of gastrotin,paeoniflorin and blood vessel diastolic rate in vitro was studied,the results were binomial equation fitting,by using the response surface method to predict the best water extraction process. Results: The predictive value of the binomial fitting equation was relatively low. In the best extraction technology conditions( soaking time for 1. 5 hours,ten times the amount of water,extraction of 1 hour every time,extraction of twice),the mean content of gastrotin was 0. 59 mg·g-1,the average content of paeoniflorin was 6. 35 mg·g-1,and the average blood vessel diastolic rate in vitro was 71. 03%. Conclusion: The method is stable and convenient and it can optimize the extraction process of the Dishaojudong granules accurately and rapidly.
Objective: This paper aims to optimize the extraction process of Dishaojudong granule by using the Box-Behnken response surface method. Methods: The effects of immersion time,extraction time,extraction multiples and extraction times on the content of gastrotin,paeoniflorin were studied by single factor test. Based on the results of single factor,the effects of the study on the time of immersion,the time of extraction and the extraction multiples were studied. The effect of the three factors on the content of gastrotin,paeoniflorin and blood vessel diastolic rate in vitro was studied,the results were binomial equation fitting,by using the response surface method to predict the best water extraction process. Results: The predictive value of the binomial fitting equation was relatively low. In the best extraction technology conditions( soaking time for 1. 5 hours,ten times the amount of water,extraction of 1 hour every time,extraction of twice),the mean content of gastrotin was 0. 59 mg·g-1,the average content of paeoniflorin was 6. 35 mg·g-1,and the average blood vessel diastolic rate in vitro was 71. 03%. Conclusion: The method is stable and convenient and it can optimize the extraction process of the Dishaojudong granules accurately and rapidly.
引文
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[2]刘力生.临床高血压病学[M].北京:北京大学医学出版社,2015.
[3]彭珍香,黄海定,邓时贵.芍药苷对大鼠离体胸主动脉血管环的作用及其机制[J].中国实验方剂学杂志,2011,17(7):190-194.
[4]谢世虎.天麻的鉴定和药理作用研究[J].中国卫生产业,2016,13(21):167-169.
[5]杨荣艳. HPLC测定通络痹痛丸中天麻素的含量[J].中国现代中药,2011,13(3):36-37.
[6]国家药典委员会.中华人民共和国药典:一部[M].北京:中国医药科技出版社,2015:105.
[7]刘厂辉,李新,陈锋,等. ERK1/2参与自发性高血压大鼠离体血管环对apelin-13舒张反应性降低作用[J].生物化学与生物物理进展,2009,36(12):1578-1588.
[8]贾国栋,崔文玉,龙超良,等.盐酸埃他卡林对大鼠主动脉血管环和尾动脉血管条的选择性舒张作用[J].中国临床康复,2004,8(3):443-445.