UPLC-Q-TOF-MS法同时测定化妆品中14种植物提取物标识成分
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摘要
建立了化妆品中添加植物提取物标识成分的超高效液相色谱-高分辨质谱(UPLC-Q-TOF-MS)测定方法,能同时测定积雪草苷、羟基积雪草苷、木犀草苷等14种标识成分。样品经有机溶剂超声提取后,提取液高速离心处理,经C18色谱柱分离,电喷雾-四极杆-飞行时间质谱检测。14种标识成分的检出限在0.01～0.025 mg/L之间,在膏霜基质中的加标回收率为61.3%～115.1%,相对标准偏差小于16.6%(n=6)。该方法适用于化妆品中添加植物提取物标识成分的定性鉴定和含量测定,方法准确、快速、灵敏度高,为化妆品的高通量快速筛查提供了实用可靠的技术手段。
A method based on ultra-high-performance liquid chromatography tandem high resolution mass spectrometry(UPLC-Q-TOF-MS) had been developed and validated for simultaneous determination of 14 indicative components in plant extracts added to cosmetics,such as Asiaticoside, Madecassoside, Cynaroside, etc. The samples were extracted by organic solvent with ultrasonic method at ?rst, then the obtained extracts were conducted fast speed centrifugation, separated on C18 column and detected by ESI-Q-TOF-MS analysis. The results showed that the limits of detection of 14 indicative components in cosmetics were 0.01 to 0.025 mg/L, the average recovery in cream matrix was 61.3 % to 115.1 %, the relative standard deviation(RSD) was less than 16.6%(n = 6). This method is accurate, rapid and high sensitive, which can be applied to qualitative identi?cation and quantitative determination of the indicative components in cosmetics with plant extracts. It may serve as a reliable and practical technology with rapid high-throughput screening for cosmeceuticals.
A method based on ultra-high-performance liquid chromatography tandem high resolution mass spectrometry(UPLC-Q-TOF-MS) had been developed and validated for simultaneous determination of 14 indicative components in plant extracts added to cosmetics,such as Asiaticoside, Madecassoside, Cynaroside, etc. The samples were extracted by organic solvent with ultrasonic method at ?rst, then the obtained extracts were conducted fast speed centrifugation, separated on C18 column and detected by ESI-Q-TOF-MS analysis. The results showed that the limits of detection of 14 indicative components in cosmetics were 0.01 to 0.025 mg/L, the average recovery in cream matrix was 61.3 % to 115.1 %, the relative standard deviation(RSD) was less than 16.6%(n = 6). This method is accurate, rapid and high sensitive, which can be applied to qualitative identi?cation and quantitative determination of the indicative components in cosmetics with plant extracts. It may serve as a reliable and practical technology with rapid high-throughput screening for cosmeceuticals.
引文
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[2]李杨杰,赵祥龙,岑立怡,等.高分辨质谱对化妆品中非法添加标准外糖皮质激素的快速筛查及应用[J].香料香精化妆品,2018(1):58-62.
[3]刘丁,杨晋青,赵艳菊,等.液相色谱-串联质谱法测定化妆品中新型防腐剂月桂酰精氨酸乙酯盐酸盐[J].香料香精化妆品,2018(2):53-58.
[4]马强,白桦,王超,等.超高效液相色谱-四极杆-飞行时间质谱法快速筛查化妆品中18种香豆素类化合物[J].分析测试学报,2014(3):248-255.
[5]林黎,张毅,涂小珂,等.液相色谱-串联质谱法同时测定化妆品中的25种喹诺酮类药物[J].色谱,2015(3):275-281.
[6]申潜,余露山,周慧,等.应用反相高效液相色谱法同时测定积雪草提取物中羟基积雪草苷、积雪草苷及其苷元[J].浙江大学学报(医学版),2014(2):135-140.
[7]刘璇,毕肖林,狄留庆,等.丹参提取物中6种活性成分UPLC测定方法的建立及其提取方法比较研究[J].中药材,2013(1):70-72.
[8]席绍峰,李慧勇,谭建华,等.SPE-UPLC-Q-TOF MS测定育发化妆品中人参和甘草类功效成分[J].质谱学报,2015(4):341-349.
[9]陈秋竹,林瑞超,王钢力,等.金银花提取物化学成分研究[J].中药材,2010(6):920-922.
[10]蒋伟,王长虹,王峥涛.当归提取物的制备工艺及其质量控制[J].华西药学杂志,2010(1):87-89.
[11]路新华,梁鸿,赵玉英.当归中藁苯内酯类化合物的分离与结构鉴定[J].中国中药杂志,2003(5):43-45.
[12]张百霞,周凤琴,郭庆梅.金银花中黄酮类化合物的研究进展[J].中国试验方剂学杂志,2012,18(23):349-352.