北京生菜黑斑病病原菌鉴定
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摘要
【目的】生菜黑斑病是生菜的重要病害之一,在生产上影响生菜质量并导致减产。目前报道的生菜黑斑病病原菌种类较多,为了明确引起北京地区生菜黑斑病的病原菌种类,从北京市昌平区生菜种植温室采集生菜黑斑病病样,进行病原菌的分离培养、致病性测定与鉴定。【方法】采用的病原菌鉴定的主要方法为形态学鉴定和分子生物学鉴定。通过显微镜观察病原菌的形态特征,并对病原菌DNA的ITS部分序列进行分析。【结果】结果表明:引起生菜黑斑病的病原菌为互隔链格孢Alternaria alternata。【结论】研究结果可以为生菜黑斑病的相关研究以及今后生产上相关生菜病害的防治提供理论依据。
Lettuce black spot is one of important diseases infecting lettuce,which affects the quality and reduces the yield of lettuce.At present,there are many kinds of pathogens of lettuce black spot reported.In order to clarify the pathogen causing lettuce black spot in Beijing,the diseased lettuce leaves which had the similar symptom of lettuce black spot were collected from the greenhouse in Changping district of Beijing and the pathogen was isolated and cultured.The pathogenicity of the isolated pathogen was tested by inoculated on healthy lettuce leaves.The isolated pathogen was identified by morpholog and molecular biology.The morphological characteristics of the pathogen was observed by microscope,and the ITS partial sequence of the pathogen DNA was analyzed.The results showed that the pathogen causing lettuce black spot was Alternaria alternata.The results could provide theoretical basis for the prevention and control of lettuce black spot and other related lettuce diseases in future research.
Lettuce black spot is one of important diseases infecting lettuce,which affects the quality and reduces the yield of lettuce.At present,there are many kinds of pathogens of lettuce black spot reported.In order to clarify the pathogen causing lettuce black spot in Beijing,the diseased lettuce leaves which had the similar symptom of lettuce black spot were collected from the greenhouse in Changping district of Beijing and the pathogen was isolated and cultured.The pathogenicity of the isolated pathogen was tested by inoculated on healthy lettuce leaves.The isolated pathogen was identified by morpholog and molecular biology.The morphological characteristics of the pathogen was observed by microscope,and the ITS partial sequence of the pathogen DNA was analyzed.The results showed that the pathogen causing lettuce black spot was Alternaria alternata.The results could provide theoretical basis for the prevention and control of lettuce black spot and other related lettuce diseases in future research.
引文
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[17]胡晓莉,蒋彩虹,耿锐梅,等.山东部分地区烟草赤星病病原菌的形态学与分子生物学鉴定[J].江苏农业科学,2018,46(11):82-86
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[2]陈青君,韩莹琰,谷建田,等.叶用莴苣种质资源的主要农艺性状鉴定与耐热性评价[J].中国蔬菜,2011(20):20-27
[3]Moura L D O,Carlos L D A,Moura L D O,et al.Physicochemical characteristics of purple lettuce harvested at different ages[J].Revists Caatinga,2016,29(2):489-495
[4]Liu D,LiH,WangY,et al.How Exogenous selenium affects anthocyanin accumulation and biosynthesis related gene expression in purple lettuce[J].Polish Journal of Environmental Studies,2017,26(2):717-722
[5]燕存起,燕敦方,刘军,等.莴苣常见病害的识别与防治方法[J].安徽农学通报,2008,14(10):76;84
[6]Nordskog,Nrstad,Ragnhild,et al.Fungal pathogens in Norwegian lettuce fields[J].Acta Agriculture Scandinavica Section B Soil and Plant Science,2008,58(2):230-235
[7]王恒亮.蔬菜病虫害诊治原色图谱[M].北京:中国农业科学技术出版社,2013
[8]李惠明,赵康,赵胜荣,等.蔬菜病虫害诊断与防治实用手册[M].上海:上海科学技术出版社,2012
[9]李金堂.蔬菜病虫害诊治图鉴[M].泰安:山东科学技术出版社,2012
[10]郭润婷,石延霞,赵倩,等.莴笋链格孢叶斑病病原鉴定[J].植物病理学报,2018,48(3):418-422
[11]席中刚,游景茂,郑露,等.湖北省黄连叶斑病病原鉴定及其生物学特性[J].植物保护学报,2018,45(2):315-321
[12]李彦忠,聂红霞.甘肃省红豆草病原真菌鉴定及病害发生动态调查[J].植物保护学报,2016,43(2):222-232
[13]吕佩珂.绿叶类蔬菜病虫害诊治原色图鉴[M].北京:化学工业出版社,2013
[14]Davis R M,Subbarao K V,Raid R N,et al.Compendium of lettucediseases[J].The Mycologist,1999,13(1):1474-0605
[15]Shtienberg D,Blachinsky D,Ben H G,Effects of growing season and fungicide type on the development of Alternariasolani and on potato yield[J].Plant Disease,1996,80(9):994-998
[16]王雅静,李郁婷,刘泉,等.棉花轮纹斑病研究进展及防控策略[J].植物保护,2018(4):9-14
[17]胡晓莉,蒋彩虹,耿锐梅,等.山东部分地区烟草赤星病病原菌的形态学与分子生物学鉴定[J].江苏农业科学,2018,46(11):82-86
[18]王婧,翟伟卜,高环,等.链格孢引起的病害严重危害农作物生产并危及农产品安[J].植物保护,2017,43(4):9-15