Survivin、Bcl-2、HPV16/18在宫颈癌中的表达及相关性研究
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摘要
目的:探讨Survivin、Bcl-2、HPV16/18在宫颈癌中的表达及相关性。方法:收集宫颈上皮内瘤变患者78例(CIN组)、宫颈癌患者40例(宫颈癌组)、宫颈正常的妇科其他疾病患者(正常宫颈组)40例的宫颈组织标本,以原位杂交法检测Survivin mRNA、HPV16/18的表达,以免疫组化法检测Bcl-2表达。结果:CIN组、宫颈癌组宫颈组织中Survivin、Bcl-2、HPV16/18阳性表达明显高于正常宫颈组(P<0.05),宫颈癌组高于CIN组(P<0.05);Survivin、Bcl-2在正常宫颈组织中不表达或表达强度为弱阳性,在宫颈癌组织中以强阳性表达多见,不同宫颈病变组织中Survivin、Bcl-2阳性表达强度明显不同(P<0.05);Survivin和Bcl-2阳性表达率在不同CIN组织、不同宫颈癌组织分化程度、临床分期、淋巴结转移中阳性表达率不同(P<0.05),而HPV16/18阳性表达率在不同CIN组织、不同宫颈癌组织分化程度、临床分期、组织类型、肿瘤外形、淋巴结转移等方面的阳性表达率均无明显不同(P>0.05);Survivin的阳性表达率与Bcl-2及HPV16/18阳性表达率间具有正相关性(P<0.05)。结论:Survivin、Bcl-2、HPV16/18在不同宫颈病变组织中表达不同,宫颈癌组织中阳性表达明显上调,提示可能与宫颈癌的发生及发展关系密切。
Objective: To explore expressions of Survivin,Bcl- 2,HPV16/18 in cervical cancer and the correlation. Methods: 78 cases of cervical intraepithelial neoplasia( CIN),40 cases of cervical cancer,40 patients with normal cervical were collected,in situ hybridization method was used to detect Survivin mRNA,expression of HPV16 /18,with immunohistochemical method to detect the Bcl- 2 expression. Results: Expressions of Survivin,Bcl- 2,HPV 16 /18 in CIN and cervical cancer groups were significantly higher than those of normal cervix group( P < 0. 05),above indexes of cervical cancer group were higher than those of CIN group( P < 0. 05). Survivin and Bcl- 2 were not expressed in normal cervical tissue or expressing intensity weak positive,strongly positive expressed in cervical cancer tissue. There were different expressions of Survivin and Bcl- 2 in different cervical lesion( P < 0. 05). There were differences in Survivin and Bcl- 2 positive expression rate among different CIN tissue,cervical cancer tissue differentiation degree,clinical stage,lymph node metastasis( P < 0. 05). There were no differences in HPV 16 /18 expression among different CIN,cervical cancer tissue differentiation degree, clinical stage,tissue type,shape of tumor,lymph node metastasis subgroup( P > 0. 05). There were positive correlations between Survivin positive expression rate and Bcl- 2 and HPV16 /18 positive expression rate( P < 0. 05). Conclusion: There are different expressions of Survivin,Bcl- 2,HPV16 /18 in different cervical lesions and Survivin,Bcl- 2,HPV16 /18 positive expression are obviously up- regulated in cervical cancer tissue,which shows that their positive expressions may be closely associated with the occurrence and development of cervical cancer.
Objective: To explore expressions of Survivin,Bcl- 2,HPV16/18 in cervical cancer and the correlation. Methods: 78 cases of cervical intraepithelial neoplasia( CIN),40 cases of cervical cancer,40 patients with normal cervical were collected,in situ hybridization method was used to detect Survivin mRNA,expression of HPV16 /18,with immunohistochemical method to detect the Bcl- 2 expression. Results: Expressions of Survivin,Bcl- 2,HPV 16 /18 in CIN and cervical cancer groups were significantly higher than those of normal cervix group( P < 0. 05),above indexes of cervical cancer group were higher than those of CIN group( P < 0. 05). Survivin and Bcl- 2 were not expressed in normal cervical tissue or expressing intensity weak positive,strongly positive expressed in cervical cancer tissue. There were different expressions of Survivin and Bcl- 2 in different cervical lesion( P < 0. 05). There were differences in Survivin and Bcl- 2 positive expression rate among different CIN tissue,cervical cancer tissue differentiation degree,clinical stage,lymph node metastasis( P < 0. 05). There were no differences in HPV 16 /18 expression among different CIN,cervical cancer tissue differentiation degree, clinical stage,tissue type,shape of tumor,lymph node metastasis subgroup( P > 0. 05). There were positive correlations between Survivin positive expression rate and Bcl- 2 and HPV16 /18 positive expression rate( P < 0. 05). Conclusion: There are different expressions of Survivin,Bcl- 2,HPV16 /18 in different cervical lesions and Survivin,Bcl- 2,HPV16 /18 positive expression are obviously up- regulated in cervical cancer tissue,which shows that their positive expressions may be closely associated with the occurrence and development of cervical cancer.
引文
1 白婷.宫颈癌患者血清中HPV检测的结果分析[J].中国妇幼保健,2013,28(18):2898-2899.
2 朱燕,杨其昌,刘宏斌,等.骨桥蛋白、survivin及bcl-1在宫颈病变中的表达及临床意义[J].肿瘤防治研究,2013,40(1):83-86.
3 Xue Y,An R,Zhang D,et al.Detection of survivin expression in cervical cancer cells using molecular beacon imaging:new strategy for the diagnosis of cervical cancer[J].Eur J Obstet Gynecol Reprod Biol,2011,159(1):204-208.
4 Liu L,Yu X,Guo X,et al.miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2[J].Mole Med Rep,2012,5(3):753-760.
5 朱华,施铮铮,杨孝军,等.Survivin、Bcl-2、HPV16/18在宫颈上皮内瘤变及宫颈癌中的表达及临床意义[J].实用妇产科杂志,2010,26(1):57-60.
6 张文渊,黄筱纮,平金良.Survivin、Fas在宫颈癌中的表达及意义[J].肿瘤学杂志,2013,19(8):605-609.
7 常丽琴,吴素慧,李超,等.Ki67、Survivin早期宫颈癌中表达与预后相关性分析[J].中国医药科学,2013,3(7):20-22.
8 朱华,施铮铮,张虎详,等.宫颈上皮内瘤变和宫颈癌组织中Survivin与Bcl-2的表达及相关性[J].温州医学院学报,2010,40(1):23-26.
9 Zhang L,Zhang S.Modulating Bcl‐2 family proteins and caspase‐3 in induction of apoptosis by paeoniflorin in human cervical cancer cells[J].Phytother Res,2011,25(10):1551-1557.
10 Naucler P,Ryd W,Trnberg S,et al.Efficacy of HPV DNA testing with cytology triage and/or repeat HPV DNA testing in primary cervical cancer screening[J].J Nat Cancer Ins,2009,101(2):88-99.
2 朱燕,杨其昌,刘宏斌,等.骨桥蛋白、survivin及bcl-1在宫颈病变中的表达及临床意义[J].肿瘤防治研究,2013,40(1):83-86.
3 Xue Y,An R,Zhang D,et al.Detection of survivin expression in cervical cancer cells using molecular beacon imaging:new strategy for the diagnosis of cervical cancer[J].Eur J Obstet Gynecol Reprod Biol,2011,159(1):204-208.
4 Liu L,Yu X,Guo X,et al.miR-143 is downregulated in cervical cancer and promotes apoptosis and inhibits tumor formation by targeting Bcl-2[J].Mole Med Rep,2012,5(3):753-760.
5 朱华,施铮铮,杨孝军,等.Survivin、Bcl-2、HPV16/18在宫颈上皮内瘤变及宫颈癌中的表达及临床意义[J].实用妇产科杂志,2010,26(1):57-60.
6 张文渊,黄筱纮,平金良.Survivin、Fas在宫颈癌中的表达及意义[J].肿瘤学杂志,2013,19(8):605-609.
7 常丽琴,吴素慧,李超,等.Ki67、Survivin早期宫颈癌中表达与预后相关性分析[J].中国医药科学,2013,3(7):20-22.
8 朱华,施铮铮,张虎详,等.宫颈上皮内瘤变和宫颈癌组织中Survivin与Bcl-2的表达及相关性[J].温州医学院学报,2010,40(1):23-26.
9 Zhang L,Zhang S.Modulating Bcl‐2 family proteins and caspase‐3 in induction of apoptosis by paeoniflorin in human cervical cancer cells[J].Phytother Res,2011,25(10):1551-1557.
10 Naucler P,Ryd W,Trnberg S,et al.Efficacy of HPV DNA testing with cytology triage and/or repeat HPV DNA testing in primary cervical cancer screening[J].J Nat Cancer Ins,2009,101(2):88-99.