模拟失重48h后血管内皮细胞长链非编码RNA的差异表达分析
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摘要
目的探究模拟失重48h后人脐静脉内皮细胞(HUVECs)中长链非编码RNA(lncRNAs)的表达差异。方法采用高通量测序技术检测HUVECs模拟失重48h组与水平静置对照组中lncRNAs的差异表达,对部分差异表达的lncRNAs进行PCR验证。结果差异表达lncRNAs共37个,模拟失重48h处理后,有22个lncRNAs显著上调(P<0.05),15个lncRNAs显著下调(P<0.05)。PCR结果表明,有4个lncRNAs的变化趋势与测序结果一致。结论模拟失重48h后HUVECs中lncRNAs的表达发生明显变化,lncRNAs的差异表达可为探索模拟失重下血管内皮细胞功能改变的发生机制提供依据。
Objective To explore the differential expression of long non-coding RNAs(lncRNAs)in human umbilical vein endothelial cells(HUVECs)after 48 hsimulated weightlessness.Methods High throughput sequencing technology was used to detect the differential expression of lncRNAs between48 hsimulated weightlessness groups and horizontal standing control groups.Expression levels of some differentially-expressed lncRNAs were validated by PCR.Results A total of 37 differentially-expressed lncRNAs were identified.After 48 hsimulated weightlessness,22 lncRNAs were up-regulated(P<0.05)and 15 down-regulated significantly(P<0.05).PCR analysis of four selected lncRNAs confirmed the results of RNA-sequencing.Conclusion The expression levels of lncRNAs in HUVECs are altered significantly after 48 hsimulated weightlessness.Differentially-expressed lncRNAs may provide evidence for exploring mechanisms of functional changes in human vascular endothelial cells under simulated weightlessness.
Objective To explore the differential expression of long non-coding RNAs(lncRNAs)in human umbilical vein endothelial cells(HUVECs)after 48 hsimulated weightlessness.Methods High throughput sequencing technology was used to detect the differential expression of lncRNAs between48 hsimulated weightlessness groups and horizontal standing control groups.Expression levels of some differentially-expressed lncRNAs were validated by PCR.Results A total of 37 differentially-expressed lncRNAs were identified.After 48 hsimulated weightlessness,22 lncRNAs were up-regulated(P<0.05)and 15 down-regulated significantly(P<0.05).PCR analysis of four selected lncRNAs confirmed the results of RNA-sequencing.Conclusion The expression levels of lncRNAs in HUVECs are altered significantly after 48 hsimulated weightlessness.Differentially-expressed lncRNAs may provide evidence for exploring mechanisms of functional changes in human vascular endothelial cells under simulated weightlessness.
引文
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[2] Buravkova LB,Rudimov EG,Andreeva ER,et al.The ICAM-1expression level determines the susceptibility of human endothelial cells to simulated microgravity[J].J Cell Biochem,2018,119(3):2875-2885.
[3]李程飞,王永春,石菲,等.回转模拟失重对人脐静脉内皮细胞LC3、p62、p53和磷酸化p70S6K表达的影响[J].航天医学与医学工程,2016,29(5):313-317.Li CF,Wang YC,Shi F,et al.Effects of clinostat weightlessness simulation on expression of LC3,p62,p53and pp70S6Kin human umbilical vein endothelial cells[J].Space Medicine&Medical Engineering,2016,29(5):313-317.
[4] Li CF,Sun JX,Gao Y,et al.Clinorotation-induced autophagy via HDM2-p53-mTOR pathway enhances cell migration in vascular endothelial cells[J].Cell Death Dis,2018,9(2):147.
[5] Kapitonova MY,Muid S,Froemming GR,et al.Real space flight travel is associated with ultrastructural changes,cytoskeletal disruption and premature senescence of HUVEC[J].Malays J Pathol,2012,34(2):103-113.
[6] Clamp M,Fry B,Kamal M,et al.Distinguishing proteincoding and noncoding genes in the human genome[J].Proc Natl Acad Sci USA,2007,104(49):19428-19433.
[7] Zhao Z,Senturk N,Song C,et al.lncRNA PAPAS tethered to the rDNA enhancer recruits hypophosphorylated CHD4/NuRD to repress rRNA synthesis at elevated temperatures[J].Genes Dev,2018,32(11-12):836-848.
[8] Hu Z,Wang H,Wang Y,et al.Genomewide analysis and prediction of functional long noncoding RNAs in osteoblast differentiation under simulated microgravity[J].Mol Med Rep,2017,16(6):8180-8188.