西藏拉鲁湿地水体酵母菌多样性及其与理化因子相关性
|
摘要
【目的】开展拉鲁湿地水体酵母菌多样性研究,探究理化因子与酵母菌群落结构的相互关系。【方法】采用原位培养法从拉鲁湿地11个水样中分离酵母菌,应用26S r RNA D1/D2区域序列分析,并结合经典分类法对获得的菌株进行分类鉴定,运用SPSS和CANOCO软件分析酵母菌多样性及其与环境因子相关性。【结果】从拉鲁湿地水体中分离得到169株酵母菌,鉴定分属为15个属31个种。优势种为Ustilentyloma graminis和Filobasidium magnum,优势属为Naganishia、Ustilentyloma、Filobasidium和Cystofibasidium。统计分析结果表明,化学需氧量(COD)是影响拉鲁湿地水体酵母菌数量的显著因素,另外,此理化因子是影响Ustilentyloma分布的重要环境条件。【结论】西藏拉鲁湿地酵母菌资源比较丰富,且存在明显的空间异质性。
[Objective] We studied the yeast diversity in Lhalu Wetland and analyzed the influence of environmental factors on yeast community.[Methods] Yeasts were isolated by in-situ cultivation and analyzed by the D1/D2 domain of large subunit(26 S) ribosomal RNA and morphological characterization.[Results] In total 169 yeast isolates were identified to 15 genera and 31 species.Ustilentyloma graminis and Filobasidium magnumgenus were the dominating species in the wetland.Moreover,Naganishia,Ustilentyloma,Filobasidium and Cystofibasidium were the dominating genera.COD was significantly positive related to yeast abundance,and it had significant influence on Ustilentyloma.[Conclusion] Yeasts were abundant and diverse among various sites in Lhalu Wetland.
[Objective] We studied the yeast diversity in Lhalu Wetland and analyzed the influence of environmental factors on yeast community.[Methods] Yeasts were isolated by in-situ cultivation and analyzed by the D1/D2 domain of large subunit(26 S) ribosomal RNA and morphological characterization.[Results] In total 169 yeast isolates were identified to 15 genera and 31 species.Ustilentyloma graminis and Filobasidium magnumgenus were the dominating species in the wetland.Moreover,Naganishia,Ustilentyloma,Filobasidium and Cystofibasidium were the dominating genera.COD was significantly positive related to yeast abundance,and it had significant influence on Ustilentyloma.[Conclusion] Yeasts were abundant and diverse among various sites in Lhalu Wetland.
引文
[1]Dong MH,Li ZY,Zhou B,Zhou Q,Yan YP,Jin FY,Li YX,Yang LY,Li SL.Diversity of cultivable yeast in Qilu Lake in winter.Acta Microbiologica Sinica,2016,56(4):603–613.(in Chinese)董明华,李治滢,周斌,周巧,严亚萍,晋方佑,李云霄,杨丽源,李绍兰.云南高原湖泊杞麓湖冬季可培养酵母菌多样性分析.微生物学报,2016,56(4):603–613.
[2]Yan YP,Li ZY,Dong MH,Zhou Q,Jin FY,Yang LY,Li SL.Yeasts from Yangzonghai Lake in Yunnan(China):diversity and extracellular enzymes.Acta Microbiologica Sinica,2013,53(11):1205–1212.(in Chinese)严亚萍,李治滢,董明华,周巧,晋方佑,杨丽源,李绍兰.云南阳宗海酵母菌种群结构及产胞外酶测试.微生物学报,2013,53(11):1205–1212.
[3]Guo XF,Li ZY,Dong MH,Zhou B,Yang LY,Li SL.Spatial dynamics of yeast community and its relationship to environmental factors in Lake Fuxian,Yunnan province.Journal of Lake Sciences,2016,28(2):358–369.(in Chinese)郭小芳,李治滢,董明华,周斌,杨丽源,李绍兰.云南高原湖泊抚仙湖酵母菌空间分布及其与环境因子的关系.湖泊科学,2016,28(2):358–369.
[4]Zhou XL,Li ZY,Yang LY,Dong MH,Li SL.Identification of yeasts isolated from Chenghai Lake,a plateau lake in Yunnan province.Acta Microbiologica Sinica,2011,51(4):547–553.(in Chinese)周新丽,李治滢,杨丽源,董明华,李绍兰.云南程海湖酵母菌多样性及应用.微生物学报,2011,51(4):547–553.
[5]Li ZY,Zhou XL,Zhou B,Dong MH,Wang YX,Yang LY,Li SL.Diversity and extracellular enzymes of yeasts from Chenghai Lake in winter.Mycosystema,2017,36(2):177–185.(in Chinese)李治滢,周新丽,周斌,董明华,王永霞,杨丽源,李绍兰.云南程海湖冬季酵母菌多样性及胞外酶活性研究.菌物学报,2017,36(2):177–185.
[6]Zhang YL,Wang CL,Bai WQ,Wang ZF,Tu YL,Yang Jaen DG.Alpine wetlands in the Lhasa River Basin,China.Journal of Geographical Sciences,2010,20(3):375–388.
[7]Liu YY,Li F,Sun QY,Xie YH.Review on the study of soil microorganisms in wetland ecosystems.Chinese Journal of Applied&Environmental Biology,2013,19(3):547–552.(in Chinese)刘银银,李峰,孙庆业,谢永宏.湿地生态系统土壤微生物研究进展.应用与环境生物学报,2013,19(3):547–552.
[8]Huang W,Chen XT.Ecological service function and degradation reason analysis of Lhalu Wetland.Journal of West China Forestry Science,2008,37(3):41–45.(in Chinese)黄薇,陈叙图.拉鲁湿地的生态服务功能及其退化原由的分析.西部林业科学,2008,37(3):41–45.
[9]He JQ,Zhang GJ,Yue HM.Diversity and bioactivity analysis of actinomycetes isolated from Lhalu Wetland nature reserve.Journal of Microbiology,2009,(4):6–10.(in Chinese)何建清,张格杰,岳海梅.拉鲁湿地自然保护区放线菌组成分析及生物活性测定.微生物学杂志,2009,(4):6–10.
[10]De J,Wu XN,Suolang DQ,Duoji ZM,Wei EX.Study on diversity of soil bacteria in Lhalu Wetland.Journal of Tibet University(Natural Science),2014,29(2):44–50.(in Chinese)德吉,吴小妮,索朗德庆,多吉卓玛,韦恩喜.拉鲁湿地土壤细菌多样性初探.西藏大学学报(自然科学版),2014,29(2):44–50.
[11]Guo XF,Xiong N,Hao Z,Liang ZP,La B.Analysis on the correlations between yeast diversity,extracellular enzyme activity and physicochemical factors of soil in Lhalu Wetland,Lhasa.Journal of Tibet University(Natural Science),2016,31(2):1–9.(in Chinese)郭小芳,熊宁,郝兆,梁泽鹏,拉巴.拉鲁湿地土壤理化因子与酵母菌多样性及产胞外酶活性相关性分析.西藏大学学报(自然科学版),2016,31(2):1–9.
[12]Ba S,Huang X,Pu B,Ma ZX.Community characteristics of sarcodines and flagellates and water environment evaluation in Lhalu Wetlands.Wetland Science,2014,12(2):182–191.(in Chinese)巴桑,黄香,普布,马正学.拉鲁湿地肉鞭虫群落特征及其水环境评价.湿地科学,2014,12(2):182–191.
[13]Liu XM,Zhuang DF,Qu R,Wan HW,Li J,Gao YH,Xiao T.Change of Lalu Wetlands from 1989 to 2009 based on remote sensing data.Wetland Science,2013,11(4):433–437.(in Chinese)刘晓曼,庄大方,屈冉,万华伟,李静,高彦华,肖桐.基于遥感数据的1989–2009年拉鲁湿地变化研究.湿地科学,2013,11(4):433–437.
[14]Nisiotou AA,Spiropoulos AE,Nychas G-JE.Yeast community structures and dynamics in healthy and Botrytis-affected grape must fermentations.Applied and Environmental Microbiology,2007,73(21):6705–6713.
[15]Kurtzman CP,Robnett CJ.Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit(26S)ribosomal DNA partial sequences.Antonie van Leeuwenhoek,1998,73(4):331–371.
[16]Yang ZJ,Li LY,Hu BB,Lin LB,Wei YL,Ji XL,Zhang Q.Correlation between polyunsaturated fatty acids and cold adaptation of Rhodotorula glutinis.Chinese Journal of Applied&Environmental Biology,2014,20(2):233–237.(in Chinese)杨昭杰,李凌彦,胡彬彬,林连兵,魏云林,季秀玲,张琦.多不饱和脂肪酸与粘红酵母低温适应性的关系.应用与环境生物学报,2014,20(2):233–237.
[17]Fan ZQ,Li ZY,Dong MH,Zhou B,Ouyang YX,Yang LY,Li SL.Carotenoid-producing yeasts resources in Fuxian Lake,Yunnan.Microbiology China,2017,44(2):296–304.(in Chinese)樊竹青,李治滢,董明华,周斌,欧阳一星,杨丽源,李绍兰.云南抚仙湖产类胡萝卜素酵母菌的资源调查.微生物学通报,2017,44(2):296–304.
[18]Qiao FJ,Li JS,Ouyang YX,Li YY,Huang YH,Sun YJ,Chang R,Li BS.Progress in microbial oils produced by Rhodotorula glutinis.Science and Technology of Food Industry,2014,35(11):391–395.(in Chinese)乔凤杰,李炯书,欧阳亚旭,李意颖,黄永虹,孙宜君,常蓉,李博生.利用粘红酵母生产微生物油脂研究进展.食品工业科技,2014,35(11):391–395.
[19]Zhong N,Zhang ZP,Zhang H,Zhang X,Tan TW.Domestication and screening of Rhodotorula glutinis with resistance to high chemical oxygen demand(COD)starch wastewater for lipid production.Journal of Beijing University of Chemical Technology(Natural Science Edition),2013,40(1):84–88.(in Chinese)钟娜,张志平,张华,张栩,谭天伟.耐高COD淀粉废水的高产油脂粘红酵母的驯化和筛选.北京化工大学学报(自然科学版),2013,40(1):84–88.
[20]Yuan M,Dong DM,Hua XY,Zhang LH.Comparison of Correlations between COD and TOC,PV and TOC,COD and PV of different river systems in Jilin province.Scientia Geographic Sanica,2008,28(2):286–290.(in Chinese)袁懋,董德明,花修艺,张立辉.吉林省不同水系的高锰酸盐指数、化学需氧量和总有机碳的相关关系比较.地理科学,2008,28(2):286–290.
[21]Dong DM,Song X,Hua XY,Yuan M,Liang JH,Guo ZY,Liang DP.Relationship between COD and TOC of typical wasterwaters in Jilin province and mechanism and main influencing factors.Journal of Jilin University(Earth Science Edition),2012,42(5):1446–1455.(in Chinese)董德明,宋兴,花修艺,袁懋,梁建海,郭志勇,梁大鹏.吉林省典型废水COD与TOC的相关关系及其形成机制和影响因素.吉林大学学报(地球科学版),2012,42(5):1446–1455.
[2]Yan YP,Li ZY,Dong MH,Zhou Q,Jin FY,Yang LY,Li SL.Yeasts from Yangzonghai Lake in Yunnan(China):diversity and extracellular enzymes.Acta Microbiologica Sinica,2013,53(11):1205–1212.(in Chinese)严亚萍,李治滢,董明华,周巧,晋方佑,杨丽源,李绍兰.云南阳宗海酵母菌种群结构及产胞外酶测试.微生物学报,2013,53(11):1205–1212.
[3]Guo XF,Li ZY,Dong MH,Zhou B,Yang LY,Li SL.Spatial dynamics of yeast community and its relationship to environmental factors in Lake Fuxian,Yunnan province.Journal of Lake Sciences,2016,28(2):358–369.(in Chinese)郭小芳,李治滢,董明华,周斌,杨丽源,李绍兰.云南高原湖泊抚仙湖酵母菌空间分布及其与环境因子的关系.湖泊科学,2016,28(2):358–369.
[4]Zhou XL,Li ZY,Yang LY,Dong MH,Li SL.Identification of yeasts isolated from Chenghai Lake,a plateau lake in Yunnan province.Acta Microbiologica Sinica,2011,51(4):547–553.(in Chinese)周新丽,李治滢,杨丽源,董明华,李绍兰.云南程海湖酵母菌多样性及应用.微生物学报,2011,51(4):547–553.
[5]Li ZY,Zhou XL,Zhou B,Dong MH,Wang YX,Yang LY,Li SL.Diversity and extracellular enzymes of yeasts from Chenghai Lake in winter.Mycosystema,2017,36(2):177–185.(in Chinese)李治滢,周新丽,周斌,董明华,王永霞,杨丽源,李绍兰.云南程海湖冬季酵母菌多样性及胞外酶活性研究.菌物学报,2017,36(2):177–185.
[6]Zhang YL,Wang CL,Bai WQ,Wang ZF,Tu YL,Yang Jaen DG.Alpine wetlands in the Lhasa River Basin,China.Journal of Geographical Sciences,2010,20(3):375–388.
[7]Liu YY,Li F,Sun QY,Xie YH.Review on the study of soil microorganisms in wetland ecosystems.Chinese Journal of Applied&Environmental Biology,2013,19(3):547–552.(in Chinese)刘银银,李峰,孙庆业,谢永宏.湿地生态系统土壤微生物研究进展.应用与环境生物学报,2013,19(3):547–552.
[8]Huang W,Chen XT.Ecological service function and degradation reason analysis of Lhalu Wetland.Journal of West China Forestry Science,2008,37(3):41–45.(in Chinese)黄薇,陈叙图.拉鲁湿地的生态服务功能及其退化原由的分析.西部林业科学,2008,37(3):41–45.
[9]He JQ,Zhang GJ,Yue HM.Diversity and bioactivity analysis of actinomycetes isolated from Lhalu Wetland nature reserve.Journal of Microbiology,2009,(4):6–10.(in Chinese)何建清,张格杰,岳海梅.拉鲁湿地自然保护区放线菌组成分析及生物活性测定.微生物学杂志,2009,(4):6–10.
[10]De J,Wu XN,Suolang DQ,Duoji ZM,Wei EX.Study on diversity of soil bacteria in Lhalu Wetland.Journal of Tibet University(Natural Science),2014,29(2):44–50.(in Chinese)德吉,吴小妮,索朗德庆,多吉卓玛,韦恩喜.拉鲁湿地土壤细菌多样性初探.西藏大学学报(自然科学版),2014,29(2):44–50.
[11]Guo XF,Xiong N,Hao Z,Liang ZP,La B.Analysis on the correlations between yeast diversity,extracellular enzyme activity and physicochemical factors of soil in Lhalu Wetland,Lhasa.Journal of Tibet University(Natural Science),2016,31(2):1–9.(in Chinese)郭小芳,熊宁,郝兆,梁泽鹏,拉巴.拉鲁湿地土壤理化因子与酵母菌多样性及产胞外酶活性相关性分析.西藏大学学报(自然科学版),2016,31(2):1–9.
[12]Ba S,Huang X,Pu B,Ma ZX.Community characteristics of sarcodines and flagellates and water environment evaluation in Lhalu Wetlands.Wetland Science,2014,12(2):182–191.(in Chinese)巴桑,黄香,普布,马正学.拉鲁湿地肉鞭虫群落特征及其水环境评价.湿地科学,2014,12(2):182–191.
[13]Liu XM,Zhuang DF,Qu R,Wan HW,Li J,Gao YH,Xiao T.Change of Lalu Wetlands from 1989 to 2009 based on remote sensing data.Wetland Science,2013,11(4):433–437.(in Chinese)刘晓曼,庄大方,屈冉,万华伟,李静,高彦华,肖桐.基于遥感数据的1989–2009年拉鲁湿地变化研究.湿地科学,2013,11(4):433–437.
[14]Nisiotou AA,Spiropoulos AE,Nychas G-JE.Yeast community structures and dynamics in healthy and Botrytis-affected grape must fermentations.Applied and Environmental Microbiology,2007,73(21):6705–6713.
[15]Kurtzman CP,Robnett CJ.Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit(26S)ribosomal DNA partial sequences.Antonie van Leeuwenhoek,1998,73(4):331–371.
[16]Yang ZJ,Li LY,Hu BB,Lin LB,Wei YL,Ji XL,Zhang Q.Correlation between polyunsaturated fatty acids and cold adaptation of Rhodotorula glutinis.Chinese Journal of Applied&Environmental Biology,2014,20(2):233–237.(in Chinese)杨昭杰,李凌彦,胡彬彬,林连兵,魏云林,季秀玲,张琦.多不饱和脂肪酸与粘红酵母低温适应性的关系.应用与环境生物学报,2014,20(2):233–237.
[17]Fan ZQ,Li ZY,Dong MH,Zhou B,Ouyang YX,Yang LY,Li SL.Carotenoid-producing yeasts resources in Fuxian Lake,Yunnan.Microbiology China,2017,44(2):296–304.(in Chinese)樊竹青,李治滢,董明华,周斌,欧阳一星,杨丽源,李绍兰.云南抚仙湖产类胡萝卜素酵母菌的资源调查.微生物学通报,2017,44(2):296–304.
[18]Qiao FJ,Li JS,Ouyang YX,Li YY,Huang YH,Sun YJ,Chang R,Li BS.Progress in microbial oils produced by Rhodotorula glutinis.Science and Technology of Food Industry,2014,35(11):391–395.(in Chinese)乔凤杰,李炯书,欧阳亚旭,李意颖,黄永虹,孙宜君,常蓉,李博生.利用粘红酵母生产微生物油脂研究进展.食品工业科技,2014,35(11):391–395.
[19]Zhong N,Zhang ZP,Zhang H,Zhang X,Tan TW.Domestication and screening of Rhodotorula glutinis with resistance to high chemical oxygen demand(COD)starch wastewater for lipid production.Journal of Beijing University of Chemical Technology(Natural Science Edition),2013,40(1):84–88.(in Chinese)钟娜,张志平,张华,张栩,谭天伟.耐高COD淀粉废水的高产油脂粘红酵母的驯化和筛选.北京化工大学学报(自然科学版),2013,40(1):84–88.
[20]Yuan M,Dong DM,Hua XY,Zhang LH.Comparison of Correlations between COD and TOC,PV and TOC,COD and PV of different river systems in Jilin province.Scientia Geographic Sanica,2008,28(2):286–290.(in Chinese)袁懋,董德明,花修艺,张立辉.吉林省不同水系的高锰酸盐指数、化学需氧量和总有机碳的相关关系比较.地理科学,2008,28(2):286–290.
[21]Dong DM,Song X,Hua XY,Yuan M,Liang JH,Guo ZY,Liang DP.Relationship between COD and TOC of typical wasterwaters in Jilin province and mechanism and main influencing factors.Journal of Jilin University(Earth Science Edition),2012,42(5):1446–1455.(in Chinese)董德明,宋兴,花修艺,袁懋,梁建海,郭志勇,梁大鹏.吉林省典型废水COD与TOC的相关关系及其形成机制和影响因素.吉林大学学报(地球科学版),2012,42(5):1446–1455.