维管束发育相关基因sm-Nvas对水稻稻瘟病抗性分析
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摘要
[目的]探究烟草维管束发育相关基因sm-Nvas对水稻稻瘟病的抗性。[方法]构建pCAMBIA1305.1-sm-Nvas双元表达载体,利用农杆菌介导的愈伤转化法转化水稻YTB,对转基因阳性植株接种稻瘟病菌后进行抗性观察。[结果]成功构建了pCAMBIA1305.1-sm-Nvas双元超表达载体,获得了转sm-Nvas YTB植株。离体接种稻瘟病菌后转基因植株的病斑比受体品种YTB的小。[结论]转sm-Nvas基因水稻具有稻瘟病抗性。
[Objective]To investigate the resistance of tobacco vascular bundle related gene sm-Nvas to rice blast. [Method]A plant binary expression vector pCAMBIA1305.1-sm-Nvas was constructed, and transformed to recipient variety YTB by Agrobacterium-mediated callus transformation. The resistance of the transgenic positive plant was investigated after inoculation with the rice blast fungi. [Result]The pCAMBIA1305.1-sm-Nvas binary over-expression vector was successfully constructed, and the transgenic YTB with sm-Nvas was obtained. The scab of the transgenic plant was smaller than that of the receptor variety YTB after inoculation of rice blast fungi in vitro. [Conclusion]The transgenic rice with sm-Nvas gene is resistance to rice blast.
[Objective]To investigate the resistance of tobacco vascular bundle related gene sm-Nvas to rice blast. [Method]A plant binary expression vector pCAMBIA1305.1-sm-Nvas was constructed, and transformed to recipient variety YTB by Agrobacterium-mediated callus transformation. The resistance of the transgenic positive plant was investigated after inoculation with the rice blast fungi. [Result]The pCAMBIA1305.1-sm-Nvas binary over-expression vector was successfully constructed, and the transgenic YTB with sm-Nvas was obtained. The scab of the transgenic plant was smaller than that of the receptor variety YTB after inoculation of rice blast fungi in vitro. [Conclusion]The transgenic rice with sm-Nvas gene is resistance to rice blast.
引文
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[2] 杨郁文,张保龙,陈天子,等.棉花PGHNBS 启动子维管束特异表达元件的分离及功能研究[J].江苏农业学报,2012,28(6):1223-1228.
[3] 张明菊,王红梅,王书珍,等.植物对维管束病原菌的防卫反应机制研究进展[J].植物生理学报,2015,51(5):601-609.
[4] ZHANG B L,OUYANG Y N,XU J Y,et al.Cadmium remobilization from shoot to grain is related to pH of vascular bundle in rice[J].Ecotoxicol Environ Saf,2018,147:913-918.
[5] 李梦阳,彭冠云,邓彪,等.水稻维管束的研究进展[J].植物生理学报,2017,53(9):1586-1590.
[6] 杨泽峰,周勇,梁国华,等.陆生植物TAL基因的水平转移起源及对水稻维管束发育和产量性状的作用[C]//内蒙古民族大学、浙江省生物信息学学会.“农业健康与环境”组学大数据整合生物信息学研讨会论文集.杭州:浙江省科学技术协会,2017.
[7] 马玲.水稻维管束发育相关基因AVB的图位克隆与功能分析[D].重庆:西南大学,2016.
[8] SINGER S D,HILY J M,COX K D.The sucrose synthase-1 promoter from Citrus sinensis directs expression of the β-glucuronidase reporter gene in phloem tissue and in response to wounding in transgenic plants[J].Planta,2011,234(3):623-637.
[9] LIU X Q,XU X,WANG C T,et al.A novel family of gene expression induced by methyl Jasmonate in tobacco(Nicotiana tabacum)[C]//The international conference on status of plant,animal & microbe genome research XI.San Diego:[s.n],2003:833.
[10] 谭艳平,叶莲,何福收,等.烟草维管束发育相关基因Nvas启动子顺式作用元件鉴定[J].中南民族大学学报(自然科学版),2018,37(2):31-35.
[11] CHEN J Y,LI N Y,MA X F,et al.The ectopic overexpression of the cotton Ve1 and Ve2-homolog sequences leads to resistance response to verticillium wilt in Arabidopsis[J].Front Plant Sci,2017,8:844.