艾叶提取物对黄嘌呤氧化酶的抑制作用及对高尿酸血症小鼠的降尿酸作用
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摘要
以蒸馏水、无水乙醇为溶剂结合超声波辅助及传统煎煮方法对艾叶进行提取,测定各提取物总黄酮和总酚含量、自由基清除能力以及艾叶提取物对黄嘌呤氧化酶(Xanthine Oxidase,XOD)的抑制作用,并选取抑制效果较好的艾叶醇提物,利用动物实验,测定小鼠血清尿酸(UA),肌酐(Scr),尿素氮水平(BUN),肝脏XOD活性,研究艾叶提取物对高尿酸血症小鼠的降尿酸的作用。结果表明:总黄酮和总酚含量:醇提物>传统法提取物>水提物,清除自由基能力和抑制XOD作用:醇提物>传统法提取物>水提物,三种提取物对XOD的抑制作用均表现为不可逆抑制,其中传统法和醇提物对XOD的抑制类型为反竞争性抑制,水提物为混合性竞争性抑制;传统法和水提物与别嘌呤醇联合抑制作用表现为相加作用,醇提物表现为弱协同作用;艾叶醇提物对高尿酸血症小鼠的UA,Scr,BUN水平均具有降低效果,对肝脏XOD活性具有抑制作用。结论:艾叶各提取物具有较好的抑制黄嘌呤氧化酶和抗氧化作用,醇提物具有开发成为预防和治疗高尿酸血症药物的潜力。
The total flavonoids,total phenols,the inhibitory effect on xanthine oxidase(XOD) activities and free radical scavenging capacity of the extracts from Artemisia argyi by distilled water,anhydrous ethanol assisted with ultrasonic and traditional decoction were measured.Alcohol extract was selected to investigate the effect on hyperuricemia.Potassium oxazidate was used as the inducer for hyperuricemia mice.The mice were continuously fed for 7 days,and serum uric acid(UA),creatinine(Scr),urea nitrogen(BUN) level and liver XOD activity were measured.Results showed that the order of total flavonoids and total phenolics content of Artemisia argyi extracts was anhydrous ethanol assisted with ultrasonic method > traditional decoction method distilled > water method;the order of free radical scavenging capacity and XOD inhibitory activity was anhydrous ethanol assisted with ultrasonic method > traditional decoction method distilled > water method.The inhibition mechanism of the three extracts showed irreversible inhibition.The inhibition type of traditional and alcohol extracts effect on XOD is anti-competitive inhibition.The water extract effect on XOD is mixed inhibition.The type of associated inhibition with allopurinol of traditional and water extracts is additive effect.The alcohol extract is weak synergies.The level of UA,Scr and BUN in hyperuricemia mice decreased and the XOD activity of liver was inhibited.The Artemisia argyi extracts have better potential for XOD inhibitory effect and antioxidation.The alcohol extract may be developed to prevent and treat hyperuricemia.
The total flavonoids,total phenols,the inhibitory effect on xanthine oxidase(XOD) activities and free radical scavenging capacity of the extracts from Artemisia argyi by distilled water,anhydrous ethanol assisted with ultrasonic and traditional decoction were measured.Alcohol extract was selected to investigate the effect on hyperuricemia.Potassium oxazidate was used as the inducer for hyperuricemia mice.The mice were continuously fed for 7 days,and serum uric acid(UA),creatinine(Scr),urea nitrogen(BUN) level and liver XOD activity were measured.Results showed that the order of total flavonoids and total phenolics content of Artemisia argyi extracts was anhydrous ethanol assisted with ultrasonic method > traditional decoction method distilled > water method;the order of free radical scavenging capacity and XOD inhibitory activity was anhydrous ethanol assisted with ultrasonic method > traditional decoction method distilled > water method.The inhibition mechanism of the three extracts showed irreversible inhibition.The inhibition type of traditional and alcohol extracts effect on XOD is anti-competitive inhibition.The water extract effect on XOD is mixed inhibition.The type of associated inhibition with allopurinol of traditional and water extracts is additive effect.The alcohol extract is weak synergies.The level of UA,Scr and BUN in hyperuricemia mice decreased and the XOD activity of liver was inhibited.The Artemisia argyi extracts have better potential for XOD inhibitory effect and antioxidation.The alcohol extract may be developed to prevent and treat hyperuricemia.
引文
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[2]Muzychka O V,Kobzar O L,Popova A V,et al.Carboxylated aurone derivatives as potent inhibitors of xanthine oxidase[J].Bioorganic&Medicinal Chemistry,2017,25(14):3606
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[5]李英,陈君,李萍.金银花中酚酸类和黄酮类成分的黄嘌呤氧化酶抑制活性[J].中国药科大学学报,2011,42(5):407-411LI Ying,CHEN Jun,LI Ping.Inhibitory activity of the flavonoids and phenolic acids from Jinyinhua on the xanthine oxidase[J].Journal of China Pharmaceutical University,2011,42(5):407-411
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[10]陈艳,堵锡华.黄酮类化合物抑制黄嘌呤氧化酶活性的定量构效关系[J].时珍国医国药,2011,22(5):1095-1097CHEN Yan,DU Xi-hua.Quantitative relationship between xanthine-oxidase inhibition activity and the molecular structure of flavonoids[J].Lishizhen Medicine and Materia Medica Research,2011,22(5):1095-1097
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[12]Dong Y,Huang H H,Zhao M M,et al.Mechanisms underlying the xanthine oxidase inhibitory effects of dietary flavonoids galangin and pinobanksin[J].Journal of Functional Foods 2016,24:26-36
[13]周佳,张国文,胡明明.红旱莲总黄酮对黄嘌呤氧化酶抑制作用及抗氧化研究[J].食品与生物技术学报,2013,32(4):353-357ZHOU Jia,ZHANG Guo-wen,HU Ming-ming.Inhibitory effect on xanthine oxidase and antioxidant activity of total flavonoids from Hypericum ascyron L.[J].Journal of Food Science and Biotechnology,2013,32(4):353-357
[14]祁鑫,王昌禄,李风娟,等.常见蔬菜提取物对黄嘌呤氧化酶抑制作用的筛选研究[J].现代食品科技,2011,27(5):511-514QI Xin,WANG Chang-lu,LI Feng-juan,et al.Inhibitory effects of the extracts of some common vegetables on xanthine oxidase[J].Modern Food Science and Technology,2011,27(5):511-514
[15]申启荣.中药黄嘌呤氧化酶抑制剂的筛选及抑制动力学研究[D].南昌:南昌大学,2015SHEN Qi-rong.The screening and study on the inhibition kinetics of xanthine oxidase inhibitors from Chinese herbal medicine[D].Nanchang:Nanchang University,2015
[16]赵玲,陈旅翼,李赫宇.一种复合萃取物对黄嘌呤氧化酶抑制作用研究[J].食品研究与开发,2015,36(17):198-200ZHAO Ling,CHEN Lyu-yi,LI He-yu.Study on inhibition of xanthine oxidase of an extractive complex[J].Food Reaserch and Development,2015,36(17):198-200
[17]Baker J F,Krishnan E,Chen L,et al.Serum uric acid and cardiovascular disease:recent developments,and where do they leave us[J].Am J Med,2005,118(8):816-826
[18]黎莉,戴立珍,杨靖,等.中药提取物中黄嘌呤氧化酶抑制剂的筛选[J].武汉工程大学学报,2010,32(3):44-46LI Li,DAI Li-zhen,YANG Jing,et al.Study on xanthine oxidase inhibitory activity of the extracts from Chinese herbal medicines[J].Journal of Wuhan Institute of Technology,2010,32(3):44-46
[19]周凌云,李盼盼,余小红,等.小远志中糖酯类成分及其体外抗黄嘌呤氧化酶活性研究[J].中草药,2016,47(23):4146-4150ZHOU Ling-yun,LI Pan-pan,YU Xiao-hong,et al.Glycolipids from Polygala sibirica var megalopha and their in vitro inhibitory activity on xanthine oxidase[J].Chinese Traditional and Herbal Drugs,2016,47(23):4146-4150
[20]段凯莉,高明景,刘永泉,等.2种艾叶酚类化合物与挥发油成分比较[J].食品科学,2017,38(4):204-210DUAN Kai-li,GAO Ming-jing,LIU Yong-quan,et al.Phenolic compounds and essential oil compositions of Artemisia argyi leaves from two growing areas[J].Food Science,2017,38(4):204-210
[21]李真真,吕洁丽,张来宾,等.艾叶的化学成分及药理作用研究进展[J].国际药学研究杂志,2016,43(6):1059-1066LI Zhen-zhen,LYU Jie-li,ZHANG Lai-bin,et al.Chemical constituents and pharmacology activities of Artemisia argyi:Research advances[J].Journal of International Pharmaceutical Research,2016,43(6):1059-1066
[22]王新芳,董岩,孔春燕.艾蒿的化学成分及药理作用研究进展[J].时珍国医国药,2006,17(2):174-175WANG Xin-fang,DONG Yan,KONG Chun-yan.Research progress on chemical constituents and pharmacological effects of Artemisia argyi[J].Lishizhen Medicine and Materia Medica Research,2006,17(2):174-175
[23]林伟青,谢建祥,王海东.白艾提取液对高尿酸血症大鼠的实验研究[J].中华风湿病学杂志,2005,9(8):509-510LIN Wei-qing,XIE Jian-xiang,WANG Hai-dong.Experimental study on rats with hyperuricemia of extracts from white Artemisia argyi[J].Chinese Journal of Rheumatology,2005,9(8):509-510
[24]谢建祥,林伟青,王海东,等.白艾提取液对黄嘌呤氧化酶活性抑制的实验研究[R].福州,第九次全国中西医结合血瘀证及活血化瘀研究学术大会,2012XIE Jian-xiang,LIN Wei-qing,WANG Hai-dong,et al.Experimental study on xanthine oxidase activity inhibition of extracts from white Artemisia argyi[R].Fuzhou,The ninth National Conference on Integrative Medicine for Blood Stasis and Blood-activating and Stasis Research,2012
[25]Senji S,Yumi T,Yuki O.Preparation and antioxidant properties of extract of Japanese persimmom leaf tea(Kakinohacha)[J].Food Chemistry,2005,89:569-575
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