两种大鼠肝癌模型制作方法的对比研究
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摘要
目的采用Walker-256细胞,分别使用两种方法建立Wistar大鼠肝癌模型并进行对比研究。方法将30只雄性Wistar大鼠随机分为肿瘤组织研磨组、肿瘤细胞腹水悬液组、空白对照组。肿瘤组织研磨组:将Walker-256细胞接种于Wistar大鼠腋下,待肿瘤组织长出大约1cm时,将肿瘤组织取出,将肿瘤组织研磨,离心,弃去上清液,留试管下层白色肿瘤细胞进行肝脏接种;肿瘤细胞腹水悬液组:将Walker-256细胞接种于Wistar大鼠腹腔,传代2代后收集腹水,离心,弃去上清液,留试管下层白色肿瘤细胞进行肝脏接种;空白对照组:取0.9%生理盐水进行肝脏接种。结果剖检结果:肿瘤组织研磨组剖检可见大鼠肝脏出现0.2～0.5cm菜花样的占位性瘤体;肿瘤细胞腹水悬液组剖检可见大鼠肝脏出现1～2cm菜花样的占位性瘤体,组织切片显微镜下观察两组模型均可见肝癌细胞,在形态和排列上没有差别。肿瘤组织研磨组的肝小叶比较完整,肿瘤细胞腹水悬液组的肝小叶基本破坏。结论采用Walker-256细胞的肿瘤组织研磨液和肿瘤细胞腹水悬液均可建立Wistar大鼠肝癌模型;肿瘤细胞腹水悬液制作的肝癌模型的瘤体生长速度大于肿瘤组织研磨组;肿瘤细胞腹水悬液组的肝脏的损伤更为严重。
Objective To compare two methods of making liver cancer model in Wistar rats were established by Walker-256 cells.Methods 30 Wistar rats were randomly divided into three groups:Tumor tissue grinding group,tumor cell ascites suspension group and control group.Tumor tissue grinding group:Walker-256 cells were inoculated under the axilla of Wistar rats.When tumor size about 1cm,tumor tissue was removed,tumor tissue was ground,centrifuged,and supernatant was discarded.The white tumor cells were left in the lower layer of test tube for liver inoculation.In the tumor cell ascites suspension group:Walker-256 cells were inoculated into the abdominal cavity of Wistar rats,then ascites were collected after 2generations,centrifuged,and supernatant was discarded,and white tumor cells were left in the lower layer of the test tube for liver inoculation.Results The tumor tissue grinding group showed the occupying tumor of 0.2-0.5cm pattern in the liver,and the tumor cell ascites suspension group showed the occupying tumor of the 1-2cm pattern in the liver.There was no difference in morphology and arrangement between both groups.The liver lobule was intact in the tumor tissue grinding group,and the hepatic lobule was basically destroyed in the tumor cell ascites suspension group.Conclusion Tumor tissue grinding fluid of Walker-256 cells and ascites suspension of tumor cells were used to establish Wistar rat liver cancer model,and the rate of growth in tumor cells ascites suspension model was much higher than that of tumor tissue grinding group.Liver damage was more severe in the tumor cell ascites suspension group.
Objective To compare two methods of making liver cancer model in Wistar rats were established by Walker-256 cells.Methods 30 Wistar rats were randomly divided into three groups:Tumor tissue grinding group,tumor cell ascites suspension group and control group.Tumor tissue grinding group:Walker-256 cells were inoculated under the axilla of Wistar rats.When tumor size about 1cm,tumor tissue was removed,tumor tissue was ground,centrifuged,and supernatant was discarded.The white tumor cells were left in the lower layer of test tube for liver inoculation.In the tumor cell ascites suspension group:Walker-256 cells were inoculated into the abdominal cavity of Wistar rats,then ascites were collected after 2generations,centrifuged,and supernatant was discarded,and white tumor cells were left in the lower layer of the test tube for liver inoculation.Results The tumor tissue grinding group showed the occupying tumor of 0.2-0.5cm pattern in the liver,and the tumor cell ascites suspension group showed the occupying tumor of the 1-2cm pattern in the liver.There was no difference in morphology and arrangement between both groups.The liver lobule was intact in the tumor tissue grinding group,and the hepatic lobule was basically destroyed in the tumor cell ascites suspension group.Conclusion Tumor tissue grinding fluid of Walker-256 cells and ascites suspension of tumor cells were used to establish Wistar rat liver cancer model,and the rate of growth in tumor cells ascites suspension model was much higher than that of tumor tissue grinding group.Liver damage was more severe in the tumor cell ascites suspension group.
引文
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[12]沈俊杰,李鹤平,张冰,等.Wistar大鼠移植性肝癌模型的建立[J].临床放射学杂志,2011,30:112-114.
[2]李翠云,段小娴.肝癌动物模型研究进展[J].医学研究杂志,2006,35(10):71-73.
[3]李晓娟,白云峰.常用实验动物肝癌模型研究进展[J].中国比较医学杂志,2012,22(4):73-77.
[4]伍龙,汤钊猷,李雁.肝癌模型的研究进展[J].中华实验外科杂志,2009,26(6):815-816.
[5]宋祥福,吕菇,刘欣,等.两种方式接种肝癌模型的比较[J].中国比较医学杂志,2007,17(2):96-98.
[6]王书杰,韦艾凌,张永琴,等.三种大鼠移植性肝癌原位模型制作的对比研究[J].实验动物科学杂志,2012,29(2):11-14.
[7]杨新宇,徐蓓蕾,沈芸.诱发性大鼠肝癌模型的建立[J].哈尔滨商业大学学报(自然科学版),2015,31(4):401-404.
[8]谢胜学,许戈良.诱导性大鼠肝癌模型进展[J].肝胆外科杂志,2007,15(3):237-239.
[9]申凤鸽.肝癌动物模型的研究及进展[J].安徽农业科学杂志,2011,39(20):12264-12266.
[10]胡卫,陈涛.鼠移植性肝癌模型研究进展[J].河南肿瘤学杂志,2003,16(6):463-466.
[11]李琦,孟庆莉,彭永海,等.大鼠移植性肝癌模型的制作[J].第二军医大学报,2002,10(23):1074.
[12]沈俊杰,李鹤平,张冰,等.Wistar大鼠移植性肝癌模型的建立[J].临床放射学杂志,2011,30:112-114.