vimentin表达水平与肛瘘感染细菌谱以及患者临床特征的关系研究
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摘要
目的探讨vimentin(波形蛋白)表达水平与肛瘘感染细菌谱的关系,以及肛瘘标本中vimentin表达水平与患者临床特征的关系。方法回顾性分析2017年1月至2017年12月杭州市第三人民医院肛肠科住院的120例肛瘘患者临床资料,采集患者瘘管分泌物进行细菌培养,瘘管组织vimentin表达水平采用免疫组化方法检测,分析细菌谱与vimentin表达水平关系,并分析vimentin表达水平主要部位及其与患者临床特征的关系。结果在120例肛瘘患者中,瘘管分泌物细菌培养结果以大肠埃希菌(63.3%)为主,其次为肺炎克雷伯菌(18.3%),金黄色葡萄球菌(7.5%)。vimentin表达水平在大肠埃希菌组与肺炎克雷伯菌组、金黄色葡萄球菌组及其他细菌组之间相比较,差异有统计学意义(P <0.05)。高位肛瘘组vimentin表达水平低于低位肛瘘组,复杂性肛瘘组vimentin表达水平低于单纯性肛瘘组,差异均有统计学意义(均P <0.05),性别、年龄和内口方向与vimentin表达水平无关(均P> 0.05)。结论肛瘘感染以肠源性细菌感染为主,以大肠埃希菌最常见,vimentin表达水平与肛瘘位置与病变程度有关,且vimentin可能参与了肛瘘的发生发展过程。
Objectives To investigate the relationship between vimentin expression and bacterial spectrum of anal fistula infection and the relationship between vimentin expression and clinical characteristics of patients. Methods This was a retrospective analysis of clinical data of 120 hospitalized patients with anal fistula treated at the Department of Anal and Intestinal Surgery,The Third Hangzhou People's Hospital, between January 2017 and December 2012. Bacterial spectrum of anal fistula infection was analyzed using bacteria culture of secretion of fistula. The expression of vimentin in fistula tissue was analyzed using immunohistochemical method. We analyzed the relationship between bacterial spectrum and vimentin expression and the relationship between vimentin expression and clinical characteristics of patients. Results Among the 120 patients with anal fistula, culture showed the main bacteria were Escherichia coli(63.3%), Klebsiella pneumoniae(18.3%) and Staphylococcus aureus(7.5%). Vimentin expression was significantly different among Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and other bacteria(P < 0.05). Vimentin expression was significantly lower in patients with high fistula than with low fistula, and significantly lower in complex fistula than in simple fistula(P < 0.05). Sex, age, and direction of internal opening of fistula did not affect vimentin expression(P > 0.05). Conclusion Bacterial infection in fistula were mostly intestinal bacteria, with Escherichia coli being the most common. The expression of vimentin is correlated with the location and severity of fistula. It was possible that vimentin contributes to the occurrence and development of fistula.
Objectives To investigate the relationship between vimentin expression and bacterial spectrum of anal fistula infection and the relationship between vimentin expression and clinical characteristics of patients. Methods This was a retrospective analysis of clinical data of 120 hospitalized patients with anal fistula treated at the Department of Anal and Intestinal Surgery,The Third Hangzhou People's Hospital, between January 2017 and December 2012. Bacterial spectrum of anal fistula infection was analyzed using bacteria culture of secretion of fistula. The expression of vimentin in fistula tissue was analyzed using immunohistochemical method. We analyzed the relationship between bacterial spectrum and vimentin expression and the relationship between vimentin expression and clinical characteristics of patients. Results Among the 120 patients with anal fistula, culture showed the main bacteria were Escherichia coli(63.3%), Klebsiella pneumoniae(18.3%) and Staphylococcus aureus(7.5%). Vimentin expression was significantly different among Escherichia coli, Klebsiella pneumoniae, Staphylococcus aureus and other bacteria(P < 0.05). Vimentin expression was significantly lower in patients with high fistula than with low fistula, and significantly lower in complex fistula than in simple fistula(P < 0.05). Sex, age, and direction of internal opening of fistula did not affect vimentin expression(P > 0.05). Conclusion Bacterial infection in fistula were mostly intestinal bacteria, with Escherichia coli being the most common. The expression of vimentin is correlated with the location and severity of fistula. It was possible that vimentin contributes to the occurrence and development of fistula.
引文
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[15]BARRIENTOS S,STOJADINOVIC O,GOLINKO M S,et al.Growth factors and cytokines in wound healing[J].Wound Repair Regen,2008,16(5):585-601.
[16]SHIGYO M,TOHDA C.Extracellular vimentin is a novel axonal growth facilitator for functional recovery in spinal cord-injured mice[J].Sci Rep,2016,6:28293.
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[19]采虹,戴功建.肛瘘术后复发危险因素探讨[J].中国医学工程,2015,23(11):30-33.
[2]EISENHAMMER S.The internal anal sphincter and the anorectal abscess[J].Surg Gynecol Obstet,1956,103(4):501-506.
[3]GOLIGHER J C,ELLIS M,PISSIDIS A G.A critique of anal glandular infection in the aetiology and treatment of idiopathic anorectal abscesses and fistulas[J].Br J Surg,1967,54(12):977-983.
[4]郑毅,崔金杰.肛瘘的诊治现状[J].中国临床医生,2011,39(4):11-13.
[5]张玉茹,李非,刘连成.肛瘘术后复发危险因素分析[J].临床外科杂志,2013,21(8):623-625.
[6]李国炜,李海洋.波形蛋白的生物学功能及相关病理的研究进展[J].医学综述,2018,24(7):1273-1278.
[7]董海峰,郑英斌,庞志刚.E-钙黏蛋白与波形蛋白在胃癌中的表达及意义[J].中华实用诊断与治疗杂志,2018,32(6):580-582.
[8]EL-TAWIL A M.Mechanism of non-specific-fistula-in-ano:hormonal aspects-review[J].Pathophysiology,2012,19(1):55-59.
[9]李嘉钦,杨巍,黄志坚.肛瘘患者临床特征及复发危险因素分析[J].中华胃肠外科杂志,2016,19(12):1370-1374.
[10]陆坚,徐照秀,吴帅军.近3年肛周脓肿及肛瘘感染病原菌分布及耐药性分析[J].国际检验医学杂志,2015,36(27):3189-3191.
[11]WANG N,STAMENOVIC D.Mechanics of vimentin intermediate filaments[J].J Muscle Res Cell Motil,2002,23(5-6):535-540.
[12]MENDEZ M G,KOJIMA S,GOLDMAN R D.Vimentin induces changes in cell shape,motility,and adhesion during the epithelial to mesenchymal transition[J].FASEB J,2010,24(6):1838-1851.
[13]MAK T N,BRUGGEMANN H.Vimentin in Bacterial Infections[J].Cells,2016,5(2).pii:E18.
[14]MOR-VAKNIN N,LEGENDRE M,YU Y,et al.Murine colitis is mediated by vimentin[J].Sci Rep,2013,3:1045.
[15]BARRIENTOS S,STOJADINOVIC O,GOLINKO M S,et al.Growth factors and cytokines in wound healing[J].Wound Repair Regen,2008,16(5):585-601.
[16]SHIGYO M,TOHDA C.Extracellular vimentin is a novel axonal growth facilitator for functional recovery in spinal cord-injured mice[J].Sci Rep,2016,6:28293.
[17]JORGENSEN M L,MOLLER C K,RASMUSSEN L,et al.An anti vimentin antibody promotes tube formation[J].Sci Rep,2017,7(1):3576.
[18]ECKES B,COLUCCIGUYON E,SMOLA H,et al.Impaired wound healing in embryonic and adult mice lacking vimentin[J].J Cell Sci,2000,113(13):2455-2462.
[19]采虹,戴功建.肛瘘术后复发危险因素探讨[J].中国医学工程,2015,23(11):30-33.