胶束电动毛细管色谱法测定保健品中的8种心血管保健功效成分
|
摘要
目的建立8种心血管保健功效成分(腺苷、大豆苷元、大豆苷、染料木苷、染料木素、葛根素、芦丁、淫羊藿苷)的胶束电动毛细管色谱同时检测法。方法采用50 cm×Φ50μm弹性石英毛细管为分离柱,柱温25℃,以50 mmol/L硼砂-10 mmol/L十二烷基硫酸钠(SDS)溶液为运行缓冲液,在16 kV直流电压下分离,紫外检测器检测。结果所建立的方法能在10 min内实现8种物质的基线分离,以苯甲酸为内标,各物质相对迁移时间及相对峰面积相对标准偏差(RSD)分别低于2.11%及2.16%,线性相关系数r值为0.9961～0.9996,检出限在0.076～0.835μg/ml之间。相对峰面积日内精密度≤2.75%,日间精密度≤6.90%,加标回收率在85.7%～96.5%范围内。结论本方法简便快速,灵敏准确,可用于心血管保健食品中8种功效成分的测定。
Objective The aim of this study was to develop a micellar electrokinetic capillary chromatography method for the simultaneous determination of eight functional ingredients including adenosine, puerarin, daidzin, genistin, rutin, daidzein,genistein and icariin with cardiovascular health care effect. Methods A 50 cm ×Φ50 μm elastic quartz capillary filled with binary buffer(50.0 mmol/L sodium borate-10.0 mmol/L sodium dodecyl sulfate) was used as a separation column, and the temperature was 25 ℃. The samples were separated with an ultraviolet detector under 16 KV direct current voltage. Results The established method could achieve the baseline separation of 8 substances within 10 min. The relative standard deviations of relative migration times and relative peak areas were less than 2.11% and 2.16% respectively when benzoic acid was used as the internal standard. The linear correlation coefficient was 0.9961-0.9996, and LODs was within the range of 0.076-0.835 μg/mL. The intra-and inter-day RSDs of relative peak area were less than 2.75% and 6.90%, respectively. Recoveries were in the range of 85.7%-96.5%. Conclusion This method was simple, rapid, sensitive and accurate, and could be used for the determination of 8 effective components in cardiovascular healthy foods.
Objective The aim of this study was to develop a micellar electrokinetic capillary chromatography method for the simultaneous determination of eight functional ingredients including adenosine, puerarin, daidzin, genistin, rutin, daidzein,genistein and icariin with cardiovascular health care effect. Methods A 50 cm ×Φ50 μm elastic quartz capillary filled with binary buffer(50.0 mmol/L sodium borate-10.0 mmol/L sodium dodecyl sulfate) was used as a separation column, and the temperature was 25 ℃. The samples were separated with an ultraviolet detector under 16 KV direct current voltage. Results The established method could achieve the baseline separation of 8 substances within 10 min. The relative standard deviations of relative migration times and relative peak areas were less than 2.11% and 2.16% respectively when benzoic acid was used as the internal standard. The linear correlation coefficient was 0.9961-0.9996, and LODs was within the range of 0.076-0.835 μg/mL. The intra-and inter-day RSDs of relative peak area were less than 2.75% and 6.90%, respectively. Recoveries were in the range of 85.7%-96.5%. Conclusion This method was simple, rapid, sensitive and accurate, and could be used for the determination of 8 effective components in cardiovascular healthy foods.
引文
[1]李昕,潘俊娴,陈士国,等.不同生长期野葛与粉葛的活性成分及体外抗氧化活性研究[J].中国食品学报,2017,17(10):220-226.
[2] Memon AF, Solangi AR, Memon SQ, et al. Simultaneous determination of quercetin, rutin, naringin, and naringenin in different fruits by capillary Zone electrophoresis[J]. Food Analytical Methods, 2017, 10(1):83-91.
[3] Dresler S, Bogucka-Kocka A, Kovacik J, et al. Separation and determination of coumarins including furanocoumarins using micellar electrokinetic capillary chromatography[J]. Talanta, 2018, 187:120-124.
[4] Chen XJ, Tu PF, Jiang Y, et al. Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE[J]. Journal of Separation Science, 2009, 32(2):275-281.
[5] Müeller LS, Da Silveira GD, PráVD, et al. Investigation of phenolic antioxidants as chemical markers in extracts of Connarus perrottetii var. Angustifolius Radlk by capillary zone electrophoresis[J]. Journal of Liquid Chromatography and Related Technologies, 2016, 39(1):13-20.
[6] Dai TT, Duan J, Li XH, et al. Determination of sulfonamide residues in food by capillary zone electrophoresis with On-Line chemiluminescence detection based on an Ag(III)complex[J]. International Journal of Molecular Sciences, 2017, 18(6):1286.
[7] Zhu P, Wang S, Wang J, et al. A capillary zone electrophoresis method for adenine nucleotides analysis in Saccharomyces cerevisiae[J]. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2016, 1008:156-163.
[8] Terabe S, Otsuka K, Ichikawa K, et al.Electrokinetic separations with micellar solutions and open-tubular capillaries[J]. Analytical Chemistry, 1984, 56(1):111-113.
[9]陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2000.
[10]张琳,刘元元,王彦,等.一种毛细管电泳系统温度控制模块的设计[J].生命科学仪器,2015,(6):40-44.
[11] Nowak PM, Spiewak K, Wozniakiewicz MA. Minimizing the impact of Joule heating as a prerequisite for the reliable analysis of metalprotein complexes by capillary electrophoresis[J]. Journal of Chromatography A, 2017, 1495:83-87.
[2] Memon AF, Solangi AR, Memon SQ, et al. Simultaneous determination of quercetin, rutin, naringin, and naringenin in different fruits by capillary Zone electrophoresis[J]. Food Analytical Methods, 2017, 10(1):83-91.
[3] Dresler S, Bogucka-Kocka A, Kovacik J, et al. Separation and determination of coumarins including furanocoumarins using micellar electrokinetic capillary chromatography[J]. Talanta, 2018, 187:120-124.
[4] Chen XJ, Tu PF, Jiang Y, et al. Effect of stability of internal standard on quantification of 15 flavonoids in Epimedium using CZE[J]. Journal of Separation Science, 2009, 32(2):275-281.
[5] Müeller LS, Da Silveira GD, PráVD, et al. Investigation of phenolic antioxidants as chemical markers in extracts of Connarus perrottetii var. Angustifolius Radlk by capillary zone electrophoresis[J]. Journal of Liquid Chromatography and Related Technologies, 2016, 39(1):13-20.
[6] Dai TT, Duan J, Li XH, et al. Determination of sulfonamide residues in food by capillary zone electrophoresis with On-Line chemiluminescence detection based on an Ag(III)complex[J]. International Journal of Molecular Sciences, 2017, 18(6):1286.
[7] Zhu P, Wang S, Wang J, et al. A capillary zone electrophoresis method for adenine nucleotides analysis in Saccharomyces cerevisiae[J]. Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2016, 1008:156-163.
[8] Terabe S, Otsuka K, Ichikawa K, et al.Electrokinetic separations with micellar solutions and open-tubular capillaries[J]. Analytical Chemistry, 1984, 56(1):111-113.
[9]陈义.毛细管电泳技术及应用[M].北京:化学工业出版社,2000.
[10]张琳,刘元元,王彦,等.一种毛细管电泳系统温度控制模块的设计[J].生命科学仪器,2015,(6):40-44.
[11] Nowak PM, Spiewak K, Wozniakiewicz MA. Minimizing the impact of Joule heating as a prerequisite for the reliable analysis of metalprotein complexes by capillary electrophoresis[J]. Journal of Chromatography A, 2017, 1495:83-87.