蟹爪叶盾蕨石油醚提取物的成分分析及抗菌活性研究
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摘要
为了研究蟹爪叶盾蕨(Neolepisorus ovatus(Bedd.)Ching f. doryopteris(Christ)Ching)石油醚提取物的化学组成及抗菌活性,采用气相色谱-质谱法分析蟹爪叶盾蕨石油醚提取物的成分,采用琼脂平板打孔法检测提取物对大肠杆菌、金黄色葡萄球菌、藤黄八叠球菌的抑制活性。从蟹爪叶盾蕨石油醚提取物中鉴定出38种化学成分,占出峰总数的92. 76%,其中豆甾-4-烯-3-酮(19. 83%)、何帕烯(16. 40%)、β-香树脂酮(14. 93%)等甾体和萜类化合物含量丰富。抑菌活性结果显示,蟹爪叶盾蕨石油醚提取物对藤黄八叠球菌的抑菌圈为(7. 78±0. 55)cm,对大肠杆菌、金黄色葡萄球菌、白色念珠菌没有抑制作用。所鉴定的化合物均首次从蟹爪叶盾蕨检测到,蟹爪叶盾蕨石油醚提取物对藤黄八叠球菌有抑制作用。此研究结果为该植物的开发利用提供了科学依据。
To study the composition and antimicrobial activity of petroleum ether extract from Neolepisorus ovatus(Bedd.)Ching f. doryopteris(Christ)Ching,components of the extract were analyzed by GC-MS,and antibacterial activities of four common pathogens were screened by fitter paper method. A total of 38 compounds were identified from the petroleum ether extract,accounted for 92. 76% of the total extract. The extracts had inhibitory effects on Micrococcus luteus with(7. 78±0. 55)cm of diameter of inhibitory zone. Moreover,the extracts did not inhibit Escherichia coli,Staphylococcus aureus and Candida albicans. The identified compounds were first detected from Neolepisorus ovatus(Bedd.)Ching f. doryopteris(Christ)Ching. The petroleum ether extract of this plant has inhibitory effect on Micrococcus luteus. The results provide scientific basis for the development and utilization of the plant.
To study the composition and antimicrobial activity of petroleum ether extract from Neolepisorus ovatus(Bedd.)Ching f. doryopteris(Christ)Ching,components of the extract were analyzed by GC-MS,and antibacterial activities of four common pathogens were screened by fitter paper method. A total of 38 compounds were identified from the petroleum ether extract,accounted for 92. 76% of the total extract. The extracts had inhibitory effects on Micrococcus luteus with(7. 78±0. 55)cm of diameter of inhibitory zone. Moreover,the extracts did not inhibit Escherichia coli,Staphylococcus aureus and Candida albicans. The identified compounds were first detected from Neolepisorus ovatus(Bedd.)Ching f. doryopteris(Christ)Ching. The petroleum ether extract of this plant has inhibitory effect on Micrococcus luteus. The results provide scientific basis for the development and utilization of the plant.
引文
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[2] Pan L T, Zhao J H, Sun Q W. Guizhou medicinal ferns[M]. Guiyang:Guizhou Science and Technology Publishing House, 2012.潘炉台,赵俊华,孙庆文.贵州药用蕨类植物[M].贵阳:贵州科技出版社, 2012.
[3] Yang J, Wu J, Li J X, et al. Preliminary study on chemical components of the Neolepisorus ovatus f. do-ryopteris(Christ)Ching[J]. Guangdong Chem Ind,2018, 45(23):53.杨俊,吴洁,李继新,等.蟹爪叶盾蕨化学成分预试验[J].广东化工, 2018, 45(23):53.
[4] Chem L J, Ma Y J, Li Y P, et al. Advance on the chemical and pharmacological studies on plants of Pyrro-sia genus[J]. Anhui Agri Sci, 2011, 39(10):5786-5787, 5789.陈丽君,马永杰,李玉鹏,等.石韦属植物化学和药理研究进展[J].安徽农业科学, 2011, 39(10):5786-5787, 5789.
[5] Li Y Q, Li H, Chen C J, et al. Preliminary study on an-tibacterial activity of Pyrrosia lingua(Thunb.)Farwell ethanol extracts[J]. Lishizhen Medicine and Materia Medica Res, 2010, 21(1):142-143.李雁群,黎桦,陈超君,等.石韦醇提物抑菌活性的初步研究[J].时珍国医国药, 2010, 21(1):142-143.
[6] Cheng D D. Comparative pharmacognosy of Pyrrosia petiolosa(Christ)Ching and Pyrrosia davidii(Baker)Ching and antibacterial and anti-inflammatory activities of extracts from Pyrrosia petiolosa[D]. Jinan:Shan-dong University of Traditional Chinese Medicine,2015.程丹丹.有柄石韦与华北石韦生药学比较及其抗菌、抗炎活性研究[D].济南:山东中医药大学, 2015.
[7] Xin X, Liu Q, Zhang Y, et al. Chemical composition and antibacterial activity of the essential oil from Pyrro-sia tonkinensis(Giesenhagen)Ching[J]. Natural Prod-uct Research, 2016, 30(7):853-856.
[8] Ma J L, Da L J, Gao S B, et al. GC-MS component analysis and antibacterial activities of the supercritical ex-tracts from Carthamus tinctorius L.[J] Anhui Agri Sci,2016, 44(4):172-174.马家麟,达洛嘉,高世宝,等.红花超临界提取物的GC-MS成分分析及抗菌活性研究[J].安徽农业科学,2016, 44(4):172-174.
[9] Wu J Y, Jia Z H, Sun J N, et al. Experimental study on the main efficacy of compound Shiwei tablets[J].Zhejiang Practical Med, 2005, 10(5):311-313.吴金英,贾占红,孙建宁,等.复方石韦片主要药效的实验研究[J].浙江实用医学, 2005, 10(5):311-313.
[10] Kim M B, Park J S, Lim S B. Antioxidant activity and cell toxicity of pressurised liquid extracts from 20 select-ed plant species in Jeju, Korea[J]. Food Chemistry,2010, 122(3):546-552.
[11] Wang B, Huang C G. Experimental study on hypogly-cemic effect of polysaccharide from Pyrrosia lingua(Thunb.)Farwell[J]. Asia-Pacific Traditional Chinese Medicine, 2008, 4(8):33-34.王兵,黄传贵.石韦多糖降血糖作用的实验研究[J].亚太传统医药, 2008, 4(8):33-34.
[12] Kim Y H, Kim K S, Han C S, et al. Inhibitory effects of natural plants of Jeju Island on elastase and MMP-1expression[J]. International Journal of Cosmetic Sci-ence, 2007, 29(6):487-488.