桂皮醛对高糖环境下MC3T3-E1成骨细胞增殖与分化的影响
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摘要
目的:观察桂皮醛对高糖作用下MC3T3-E1成骨细胞增殖与分化的影响。方法:用0(对照组)、10、20、40 mg/L的桂皮醛分别在葡萄糖(25 mmol/L)的环境下培养MC3T3-E1细胞,培养1、4、7 d后检测细胞增殖、碱性磷酸酶活性,茜素红染色观察钙化结节,鬼笔环肽染色观察细胞结构框架以及成骨相关基因骨钙蛋白(osteocalcin,OCN) mRNA表达水平。结果:桂皮醛可促进高糖环境下成骨细胞增殖及ALP活性(P<0.05);矿化结节数量增多且体积增大;实验组OCN mRNA表达水平明显上调(P<0.05);细胞骨架染色结果示:当桂皮醛浓度为10、20 mg/L时细胞铺展面积增大且细胞骨架更加清晰。结论:桂皮醛可促进高糖作用下MC3T3-E1成骨细胞的增殖与分化。
Objective: To observe the effects of cinnamaldehyde(CA) on the proliferation and differentiation of MC3 T3-E1 osteoblasts induced by high glucose. Methods: MC3 T3-E1 cells were cultured in glucose(25 mmol/L) with 0(control), 10, 20, and 40 mg/L CA. Cell proliferation, ALP activity, and alizarin red staining was measured. Morphology of the cytoskeleton was observed by confocal laser scanning microscope. Expression levels of osteogenesis related genes OCN mRNA were detected by real-time PCR. Results: CA could promote the proliferation and ALP activity of osteoblasts in high glucose environment(P<0.05). The number and the volume of mineralized nodules increased. The expression of OCN mRNA in the experimental group was significantly up-regulated(P<0.05). When the concentration of CA was 10 mg/L and 20 mg/L, the cell spreading area was increased and the cytoskeleton was clearer. Conclusion: CA can promote the proliferation and differentiation of MC3 T3-E1.
Objective: To observe the effects of cinnamaldehyde(CA) on the proliferation and differentiation of MC3 T3-E1 osteoblasts induced by high glucose. Methods: MC3 T3-E1 cells were cultured in glucose(25 mmol/L) with 0(control), 10, 20, and 40 mg/L CA. Cell proliferation, ALP activity, and alizarin red staining was measured. Morphology of the cytoskeleton was observed by confocal laser scanning microscope. Expression levels of osteogenesis related genes OCN mRNA were detected by real-time PCR. Results: CA could promote the proliferation and ALP activity of osteoblasts in high glucose environment(P<0.05). The number and the volume of mineralized nodules increased. The expression of OCN mRNA in the experimental group was significantly up-regulated(P<0.05). When the concentration of CA was 10 mg/L and 20 mg/L, the cell spreading area was increased and the cytoskeleton was clearer. Conclusion: CA can promote the proliferation and differentiation of MC3 T3-E1.
引文
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[2] Oates TW,Huynh-Ba G,Vargas A,et al.A critical review of diabetes,glycemic control,and dental implant therapy [J].Clin Oral Impl Res,2013,24 (2) :117-127.
[3] Zhu R,Liu H,Liu C,et al.Cinnamaldehyde indiabetes:a review of pharmacology,pharmacokineticsand safety [J].Pharmacol Res,2017,122:78-89.
[4] Wu Z,Weng S,Yan D,et al.Administration of cinnamaldehyde promotes osteogenesis in ovariectomized rats and differentiation of osteoblast in vitro [J].J Pharmacol Sci,2018,138(1):63-70.
[5] 苏野,邵聆.1型糖尿病小鼠下颌密质骨厚度特点的研究[J].口腔医学研究,2018,34(6):619-622.
[6] Beejmohun V ,Peytavy-Izard M ,Mignon C ,et al.Acute effect of Ceylon cinnamon extract on postprandial glycemia:alpha-amylase inhibition,starch tolerance test in rats,and randomized crossover clinical trial in healthy volunteers [J].BMC Complementary Altern Med,2014,14(1):351.
[7] Zhu R,Liu H,Liu C,et al.Cinnamaldehyde in diabetes:A review of pharmacology,pharmacokinetics and safety [J].Pharmacol Res,2017,122:78-89.
[8] Wu Z,Yan D,Xie Z,et al.Combined treatment with cinnamaldehyde and PTH enhances the therapeutic effect on glucocorticoid-induced osteoporosis through inhibiting osteoclastogenesis and promoting osteoblastogenesis [J].Biochem Biophys Res Commun,2018,505(3):945-950.
[9] Martin M,Valencia K,Zandueta C,et al.The usefulness of bone biomarkers for monitoring treatment disease:a comparative study in osteolytic and osteosclerotic bone metastasis models [J].Translational Oncology,2017,10(2) :255-261.
[10] Ferron M,Hinoi E,Karsenty G,et al.Osteocalcin differentially regulates beta cell and adipocyte gene expression and affects the development of metabolic diseases in wild-type mice [J].Proc Natl Acad Sci U S A,2008,105 (13) :5266-5270.
[11] Jones TJ,Adapala RK,Geldenhuys WJ,et al.Primary cilia regulates the directional migration and barrier integrity of endothelial cells through the modulation of hsp27 dependent actin cytoskeletal organization [J].J Cell Physiol,2012,227(1):70-76.