血管内皮生长因子在绵羊肺脏中的表达分布研究
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摘要
[目的]研究血管内皮生长因子(vascular endothelial growth factor,VEGF)在绵羊肺脏中的表达分布特征。[方法]取成年绵羊肺脏组织,制备石蜡切片,利用HE染色法观察绵羊肺脏组织的形态结构,采用免疫组织化学方法检测VEGF在绵羊肺脏组织中的分布。[结果]肺脏的各类型细胞均可见VEGF表达,在绵羊肺脏导气部的细支气管和终末细支气管的上皮细胞,呼吸部的肺泡管和呼吸性细支气管的上皮细胞,以及肺的血管内皮细胞均可检测到VEGF的强阳性表达信号。[结论]VEGF广泛分布于绵羊肺脏组织中,对其形态结构和功能的维持具有重要作用。
[Objective] To characterize the expression and distribution of vascular endothelial growth factor(VEGF) in sheep lung. [Method] The lung tissues of adult sheep were sampled to prepare the paraffin sections. The morphological structure of sheep lung tissues was observed by HE staining. The distribution of VEGF in sheep lung tissues was assessed by immunohistochemistry. [Result] VEGF expression was observed in all types of lung cells. The strong positive expression signals of VEGF were detected in epithelial cells in bronchioles and terminal bronchioles of air conduction part of the lung, epithelial cells in alveolar ductules and respiratory bronchioles of the respiratory part of lung, and the vascular endothelial cells of the lung.[Conclusion] VEGF was widely distributed in lung tissues of sheep and played an important role in the maintenance of lung morphological structure and function.
[Objective] To characterize the expression and distribution of vascular endothelial growth factor(VEGF) in sheep lung. [Method] The lung tissues of adult sheep were sampled to prepare the paraffin sections. The morphological structure of sheep lung tissues was observed by HE staining. The distribution of VEGF in sheep lung tissues was assessed by immunohistochemistry. [Result] VEGF expression was observed in all types of lung cells. The strong positive expression signals of VEGF were detected in epithelial cells in bronchioles and terminal bronchioles of air conduction part of the lung, epithelial cells in alveolar ductules and respiratory bronchioles of the respiratory part of lung, and the vascular endothelial cells of the lung.[Conclusion] VEGF was widely distributed in lung tissues of sheep and played an important role in the maintenance of lung morphological structure and function.
引文
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[13]BROWN L F,OLBRICHT S M,BERSE B,et al. Overexpression of vascular permeability factor(VPF/VEGF)and its endothelial cell receptors in delayed hypersensitivity skin reactions[J].Journal of Immunology,1995,154(6):2801-2807.
[14]YUN E J,LORIZIO W,SEEDORF G,et al. VEGF andendothelium-derived retinoic acid regulate lung vascular and alveolar development[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2016,310(4):287-298.
[15]WIECK M M,SPURRIER R G,LEVIN D E,et al. Sequestration of vascular endothelial growth factor(VEGF)induces late restrictive lung disease[J].PLoS One,2016,11(2):e0148323.
[2]COULTAS L,CHAWENGSAKSOPHAK K,ROSSANT J. Endothelial cells and VEGF in vascular development[J].Nature,2005,438(7070):937-945.
[3]BREIER G,ALBRECHT U,STERRER S,et al. Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation[J].Development,1992,114(2):521-532.
[4]佳佳.肺特异VEGF抑制对肺发育和肺功能的影响[D].长春:东北师范大学,2017.
[5]WOIK N,KROLL J. Regulation of lung development and regeneration by the vascular system[J].Cellular and Molecular Life Sciences,2015,72(14):2709-2718.
[6]COMPERNOLLE V,BRUSSELMANS K,ACKER T,et al. Loss of HIF-2αand inhibition of VEGF impair fetal lung maturation,whereas treatment with VEGF prevents fatal respiratory distress in premature mice[J].Nature Medicine,2002,8(7):702-710.
[7]ZENG X,WERT S E,FEDERICI R,et al. VEGF enhances pulmonary vasculogenesis and disrupt lung morphogenesis in vivo[J].Developmental Dynamics,1998,211(3):215-227.
[8]MANISCALCO W M,WATKINS R H,PRYHUBER G S,et al. Angiogenic factors and alveolar vasculature:Development and alterations by injury in very premature baboons[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2002,282(4):811-823.
[9]CHUNG S A,FERRARA N. Developmental and pathological angiogenesis[J].Annual Review of Cell and Developmental Biology,2010,27:563-584.
[10]GALAMBOS C,NG Y S,ALI A,et al. Defective pulmonary development in the absence of heparin-binding vascular endothelial growth factor isoforms[J].American Journal of Respiratory Cell and Molecular Biology,2002,27(2):194-203.
[11]JAKKULA M,Le CRAS T D,GEBB S,et al. Inhibition of angiogenesis decreases alveolarization in the developing rat lung[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2000,279(3):600-607.
[12]AKESON A L,CAMERON J E,Le CRAS T D,et al.Vascular endothelial growth factor-A induces prenatal neovascularization and alters bronchial development in mice[J].Pediatric Research,2005,57(1):82-88.
[13]BROWN L F,OLBRICHT S M,BERSE B,et al. Overexpression of vascular permeability factor(VPF/VEGF)and its endothelial cell receptors in delayed hypersensitivity skin reactions[J].Journal of Immunology,1995,154(6):2801-2807.
[14]YUN E J,LORIZIO W,SEEDORF G,et al. VEGF andendothelium-derived retinoic acid regulate lung vascular and alveolar development[J].American Journal of Physiology-Lung Cellular and Molecular Physiology,2016,310(4):287-298.
[15]WIECK M M,SPURRIER R G,LEVIN D E,et al. Sequestration of vascular endothelial growth factor(VEGF)induces late restrictive lung disease[J].PLoS One,2016,11(2):e0148323.