UPLC-MS/MS测定食品中18种非食用色素的研究
|
摘要
建立以超高效液相色谱-串联三重四级杆质谱同时测定辣椒酱中18种非食用色素的有效方法。样品通过MgSO4和NaCl协同乙腈振荡提取,提取液经QuEChERS(quick、easy、cheap、effective、rugged、safe的简称)填料净化后采用多反应监测(multiple reaction monitoring,MRM)模式进行测定。结果表明:18种非食用色素的定量限为6.0μg/kg～51.0μg/kg,回收率为80.2%～109.2%,相对标准偏差为2.4%～9.7%。该方法实现了多种非食用色素的同时分析,适用于打击辣椒酱中非法添加非食用色素的快速检测。
Methods to determine 18 non-edible pigments in chili sauce had been established by ultra performance liquid chromatography-mass spectrometry mass spectrometry(UPLC-MS/MS). Samples were extracted by MgSO4 and NaCl accompanied with acetonitrile first,and then purified by QuEChERS(quick,easy,cheap,effective,rugged,safe) paddings. The target compounds were detected by multiple reaction monitoring(MRM)mode finally. The experimental results showed that the quantitation limits of the 18 nonedible pigments vary from 6.0 μg/kg-51.0 μg/kg,the average recoveries ranging from 80.2 % to 109.2 %,the relative standard deviations 2.4 % to 9.7 %.This method is simple,rapid and efficient,and it can meet the requirement of simultaneous determination of non-edible pigments in chili sauce,contributing to attack illegal addition of non-edible pigments in chili sauce.
Methods to determine 18 non-edible pigments in chili sauce had been established by ultra performance liquid chromatography-mass spectrometry mass spectrometry(UPLC-MS/MS). Samples were extracted by MgSO4 and NaCl accompanied with acetonitrile first,and then purified by QuEChERS(quick,easy,cheap,effective,rugged,safe) paddings. The target compounds were detected by multiple reaction monitoring(MRM)mode finally. The experimental results showed that the quantitation limits of the 18 nonedible pigments vary from 6.0 μg/kg-51.0 μg/kg,the average recoveries ranging from 80.2 % to 109.2 %,the relative standard deviations 2.4 % to 9.7 %.This method is simple,rapid and efficient,and it can meet the requirement of simultaneous determination of non-edible pigments in chili sauce,contributing to attack illegal addition of non-edible pigments in chili sauce.
引文
[1]宋雁,李宁.食品中苏丹红的危险性评估[J].国外医学(卫生学分册),2005, 32(3):129-132
[2]陈艳美,于淼.罗丹明B染色食品对人体的危害及检测[J].科学之友, 2011(18):153
[3]中华人民共和国国家质量监督检验检疫总局. GB/T 23496-2009食品中禁用物质的检测—碱性橙染料[S].北京:中国标准出版社,2009
[4]中华人民共和国国家质量监督检验检疫总局. GB/T 19681-2005食品中苏丹红染料的检测方法高效液相色谱法[S].北京:中国标准出版社,2005
[5]中华人民共和国国家质量监督检验检疫总局. SN/T 2430-2010进出口食品中罗丹明B的检测方法[S].北京:中国标准出版社,2010
[6] Anastassiades M, Lehotay S J, Stajnbaher D, et al. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and"dispersive solid-phase extraction"for the determination of pesticide residues in produce[J].J AOAC Int, 2003, 86(2):412-431
[7]唐俊,曾凯,张敏,等.基于QuEChERS—液质联用法同时测定大米中五种新型农药残留[J].食品工业,2016,37(3):276-279
[8]陈林,温家欣,吴霞,等.改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14种非法添加工业染料[J].食品科学,2017,38(8):296-302
[9]胡侠,肖光,潘炜,等.高效液相色谱~串联质谱法同时测定辣椒粉及辣椒油中的7种罗丹明染料[J].色谱, 2010, 28(6):590-595
[10]朱程云,魏杰,董雪芳,等.改进的QuEChERS方法用于鱼肉中孔雀石绿、隐色孔雀石绿、结晶紫和隐色结晶紫的快速检测[J].色谱,2014, 32(4):419-425
[2]陈艳美,于淼.罗丹明B染色食品对人体的危害及检测[J].科学之友, 2011(18):153
[3]中华人民共和国国家质量监督检验检疫总局. GB/T 23496-2009食品中禁用物质的检测—碱性橙染料[S].北京:中国标准出版社,2009
[4]中华人民共和国国家质量监督检验检疫总局. GB/T 19681-2005食品中苏丹红染料的检测方法高效液相色谱法[S].北京:中国标准出版社,2005
[5]中华人民共和国国家质量监督检验检疫总局. SN/T 2430-2010进出口食品中罗丹明B的检测方法[S].北京:中国标准出版社,2010
[6] Anastassiades M, Lehotay S J, Stajnbaher D, et al. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and"dispersive solid-phase extraction"for the determination of pesticide residues in produce[J].J AOAC Int, 2003, 86(2):412-431
[7]唐俊,曾凯,张敏,等.基于QuEChERS—液质联用法同时测定大米中五种新型农药残留[J].食品工业,2016,37(3):276-279
[8]陈林,温家欣,吴霞,等.改良QuEChERS技术结合液相色谱-串联质谱联用法同时快速检测辣椒制品中14种非法添加工业染料[J].食品科学,2017,38(8):296-302
[9]胡侠,肖光,潘炜,等.高效液相色谱~串联质谱法同时测定辣椒粉及辣椒油中的7种罗丹明染料[J].色谱, 2010, 28(6):590-595
[10]朱程云,魏杰,董雪芳,等.改进的QuEChERS方法用于鱼肉中孔雀石绿、隐色孔雀石绿、结晶紫和隐色结晶紫的快速检测[J].色谱,2014, 32(4):419-425