红叶腺柳继代培养研究
|
摘要
以红叶腺柳(Salix chaenomeloides‘Variegata’)带芽茎段诱导出的侧芽为试验材料,研究了不同细胞分裂素、生长素、添加物、抗褐化剂及降低激素浓度等因素对红叶腺柳试管苗继代增殖及高生长的影响。结果表明,适宜的丛生芽增殖培养基为:WPM+6-BA1.0mg·L~(-1)+NAA0.1mg·L~(-1);利于试管苗伸长培养基为:WPM+6-BA0.5mg·L~(-1)+NAA0.1mg·L~(-1)+活性炭1.0g·L~(-1),添加抗褐化剂活性炭,并采用1.0mg·L~(-1)6-BA与0.5mg·L~(-1)6-BA交替使用的方法 ,在保持红叶腺柳高繁殖率的同时促进试管苗高生长,抑制茎尖坏死、玻璃化、褐化等不良现象。
Lateral buds of Salix chaenomeloides 'Variegata' were used as materials to study effect of different kinds and concentration of cytokinins、auxins、additives、browning inhibitors and reduced plant growth regulators concentration on shoot multiplication and elongation.The optimum medium for shoot multiplication is:WPM +6-BA1.0mg·L~(-1)+NAA0.1mg·L~(-1);The optimum medium for shoot Elongation is:WPM +6-BA0.5mg·L~(-1)+NAA0.1mg·L~(-1)+active carbon1.0g·L~(-1).Add active carbon,combined with using 1.0mg·L~(-1)6-BA and 0.5mg·L~(-1)6-BA alternately,which can Maintain a high multiplication value,shoot elongation without apical necrosis、vitrification and browning.
Lateral buds of Salix chaenomeloides 'Variegata' were used as materials to study effect of different kinds and concentration of cytokinins、auxins、additives、browning inhibitors and reduced plant growth regulators concentration on shoot multiplication and elongation.The optimum medium for shoot multiplication is:WPM +6-BA1.0mg·L~(-1)+NAA0.1mg·L~(-1);The optimum medium for shoot Elongation is:WPM +6-BA0.5mg·L~(-1)+NAA0.1mg·L~(-1)+active carbon1.0g·L~(-1).Add active carbon,combined with using 1.0mg·L~(-1)6-BA and 0.5mg·L~(-1)6-BA alternately,which can Maintain a high multiplication value,shoot elongation without apical necrosis、vitrification and browning.
引文
[1]郑万钧.中国树木志:第2卷[Z].北京:中国林业出版社,1985.
[2]侯元凯,刘松杨,张彦.红叶柳等彩叶树种栽培与管理[Z].北京:中国农业出版社,2008.
[3]Amo-Marco J B,Lledo M D.In vitro propagation of Salix tarraconensis Pau ex Font Quer,an endemic and threatened plant[J].In Vitro Cellular&Developmental Biology-Plant.1995,32(1):42-46.
[4]Leshem B,Sachs T.'Vitrified'Dianthus—Teratomata in vitro due to Growth Factor Imbalance[J].Annals of Botany.1985,56(5):613-617.
[5]关亚丽,陈英,黄敏仁.簸箕柳组织培养初步研究[J].海南师范大学学报(自然科学版).2009,(02):204-208.
[6]胡珊,李青.红叶腺柳组织培养及快速繁殖技术研究:2015年中国观赏园艺学术研讨会[Z].中国福建厦门:20156.
[7]王雅群,李辛晨,陈玉珍,等.北京颐和园西堤古柳组织培养体系的建立[J].林业科技开发.2009,(03):48-51.
[8]余如刚,杜雪玲,夏阳,等.旱柳Q106组织培养及快繁体系的建立[J].草原与草坪.2005,(05):59-61.
[9]马宗新,高玉祥,赵红,等.三倍体速生杨树的组织培养及应用[J].安徽农业科学.2004,(04):715-716.
[10]汤青林,牛义,王志敏,等.芋芽继代培养中激素、水解乳蛋白、碳源的调节研究[J].西南农业学报.2006,(05):928-930.
[11]李忠光,龚明.水解酪蛋白对烟草愈伤组织和悬浮培养细胞生长的促进作用[J].云南师范大学学报(自然科学版).2006,(04):60-61.
[12]Khan M,Ahmad N,Anis M.The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.:a tree of ecological importance[J].Trees.2011,25(4):577-584.
[13]王亚琴,张康健,黄江康.植物衰老的分子基础与调控[J].西北植物学报.2003,(01):182-189.
[14]陈菲,李黎,宫伟.植物组织培养的防褐化探讨[J].北方园艺.2005,(02):69.
[15]郑文静,赵海岩,杨立国.植物组织培养操作过程中的常见问题及其解决办法[J].辽宁农业科学.2001,(02):49-51.
[16]杜敬然,赵斌,李英丽,等.红掌组织培养过程中外植体褐变的研究[J].北方园艺.2010,(09):160-162.
[17]王关林,方宏筠,那杰.高活性细胞激动素TDZ在植物组织培养中的应用[J].植物学通报.1997,(03):48-54.
[18]陈凯.植物组织培养中褐变的产生机理及抑制措施[J].安徽农业科学.2004,(05):1034-1036.
[19]Hayden M R,Tyagi S C,Kolb L,et al.Vascular ossification–calcification in metabolic syndrome,type 2 diabetes mellitus,chronic kidney disease,and calciphylaxis–calcific uremic arteriolopathy:the emerging role of sodium thiosulfate[J].Cardiovascular Diabetology.2005:4.
[20]Siddique I,Anis M.Direct plant regeneration from nodal explants of Balanites?aegyptiaca L.(Del.):a valuable medicinal tree[J].New Forests.2009,37(1):53-62.
[21]郭小燕.材用优良无性系杂交柳遗传转化体系的研究[D].山东师范大学,2007.
[2]侯元凯,刘松杨,张彦.红叶柳等彩叶树种栽培与管理[Z].北京:中国农业出版社,2008.
[3]Amo-Marco J B,Lledo M D.In vitro propagation of Salix tarraconensis Pau ex Font Quer,an endemic and threatened plant[J].In Vitro Cellular&Developmental Biology-Plant.1995,32(1):42-46.
[4]Leshem B,Sachs T.'Vitrified'Dianthus—Teratomata in vitro due to Growth Factor Imbalance[J].Annals of Botany.1985,56(5):613-617.
[5]关亚丽,陈英,黄敏仁.簸箕柳组织培养初步研究[J].海南师范大学学报(自然科学版).2009,(02):204-208.
[6]胡珊,李青.红叶腺柳组织培养及快速繁殖技术研究:2015年中国观赏园艺学术研讨会[Z].中国福建厦门:20156.
[7]王雅群,李辛晨,陈玉珍,等.北京颐和园西堤古柳组织培养体系的建立[J].林业科技开发.2009,(03):48-51.
[8]余如刚,杜雪玲,夏阳,等.旱柳Q106组织培养及快繁体系的建立[J].草原与草坪.2005,(05):59-61.
[9]马宗新,高玉祥,赵红,等.三倍体速生杨树的组织培养及应用[J].安徽农业科学.2004,(04):715-716.
[10]汤青林,牛义,王志敏,等.芋芽继代培养中激素、水解乳蛋白、碳源的调节研究[J].西南农业学报.2006,(05):928-930.
[11]李忠光,龚明.水解酪蛋白对烟草愈伤组织和悬浮培养细胞生长的促进作用[J].云南师范大学学报(自然科学版).2006,(04):60-61.
[12]Khan M,Ahmad N,Anis M.The role of cytokinins on in vitro shoot production in Salix tetrasperma Roxb.:a tree of ecological importance[J].Trees.2011,25(4):577-584.
[13]王亚琴,张康健,黄江康.植物衰老的分子基础与调控[J].西北植物学报.2003,(01):182-189.
[14]陈菲,李黎,宫伟.植物组织培养的防褐化探讨[J].北方园艺.2005,(02):69.
[15]郑文静,赵海岩,杨立国.植物组织培养操作过程中的常见问题及其解决办法[J].辽宁农业科学.2001,(02):49-51.
[16]杜敬然,赵斌,李英丽,等.红掌组织培养过程中外植体褐变的研究[J].北方园艺.2010,(09):160-162.
[17]王关林,方宏筠,那杰.高活性细胞激动素TDZ在植物组织培养中的应用[J].植物学通报.1997,(03):48-54.
[18]陈凯.植物组织培养中褐变的产生机理及抑制措施[J].安徽农业科学.2004,(05):1034-1036.
[19]Hayden M R,Tyagi S C,Kolb L,et al.Vascular ossification–calcification in metabolic syndrome,type 2 diabetes mellitus,chronic kidney disease,and calciphylaxis–calcific uremic arteriolopathy:the emerging role of sodium thiosulfate[J].Cardiovascular Diabetology.2005:4.
[20]Siddique I,Anis M.Direct plant regeneration from nodal explants of Balanites?aegyptiaca L.(Del.):a valuable medicinal tree[J].New Forests.2009,37(1):53-62.
[21]郭小燕.材用优良无性系杂交柳遗传转化体系的研究[D].山东师范大学,2007.