肝癌组织CtBP1、E-钙黏蛋白的表达与临床意义
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摘要
目的:探究羧基末端结合蛋白1(CtBP1)、E-钙黏蛋白(E-cadherin)在肝癌组织中的表达情况及临床意义。方法:采用免疫组织化学染色法检测200例肝癌及癌旁组织标本CtBP1和E-钙黏蛋白的表达情况,并分析CtBP1、E-钙黏蛋白表达与肝癌临床病理特征的关系,以及肝癌组织二者表达的相关性。结果:肝癌组织CtBP1的阳性表达率明显高于癌旁组织、E-钙黏蛋白的阳性表达率明显低于癌旁组织,差异有统计学意义(P<0.05)。肝癌组织CtBP1、E-钙黏蛋白表达与年龄、性别无关(P>0.05),与TNM分期、分化程度和淋巴结转移有关(P<0.05)。肝癌组织CtBP1、E-钙黏蛋白表达呈负相关关系(r=-0.102,P<0.05)。结论:肝癌组织CtBP1和E-cadherin均呈异常表达,且二者可能存在调控关系,联合检测CtBP1、E-钙黏蛋白有望成为评估肝癌恶性生物学行为的指标。
Objective: To explore the expression and clinical value of carboxy-terminal binding protein 1(CtBP1) and E-cadherin in liver cancer. Methods: The expression of CtBP1 and E-cadherin in 200 liver cancer tissues and paracancerous tissues were detected by immunohistochemical staining. The relationships between CtBP1, E-cadherin expression and clinicopathological features of liver cancer, as well as the correlations between CtBP1 and E-cadherin in liver cancer were analyzed. Results: The positive expression rate of CtBP1 in liver cancer was significantly higher than that in paracancerous tissues, while the positive expression rate of E-cadherin was significantly lower(P<0.05). The CtBP1 and E-cadherin expression in liver cancer were not related to age, sex and tumor size(P>0.05); but related to TNM stage, differentiation and lymph node metastasis(P<0.05). In liver cancer, the CtBP1 expression was negatively correlated with E-cadherin expression(r=-0.102, P<0.05). Conclusions: CtBP1 and E-cadherin are abnormally expressed in liver cancer, and they may have regulatory relationships, so combined detection of CtBP1 and E-cadherin is expected to be an indicator for evaluating the malignant biological behavior of liver cancer.
Objective: To explore the expression and clinical value of carboxy-terminal binding protein 1(CtBP1) and E-cadherin in liver cancer. Methods: The expression of CtBP1 and E-cadherin in 200 liver cancer tissues and paracancerous tissues were detected by immunohistochemical staining. The relationships between CtBP1, E-cadherin expression and clinicopathological features of liver cancer, as well as the correlations between CtBP1 and E-cadherin in liver cancer were analyzed. Results: The positive expression rate of CtBP1 in liver cancer was significantly higher than that in paracancerous tissues, while the positive expression rate of E-cadherin was significantly lower(P<0.05). The CtBP1 and E-cadherin expression in liver cancer were not related to age, sex and tumor size(P>0.05); but related to TNM stage, differentiation and lymph node metastasis(P<0.05). In liver cancer, the CtBP1 expression was negatively correlated with E-cadherin expression(r=-0.102, P<0.05). Conclusions: CtBP1 and E-cadherin are abnormally expressed in liver cancer, and they may have regulatory relationships, so combined detection of CtBP1 and E-cadherin is expected to be an indicator for evaluating the malignant biological behavior of liver cancer.
引文
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[9]王红山,刘迎,解寒冰,等.EphA2和上皮型钙黏蛋白表达与胃癌侵袭转移的关系[J].中华胃肠外科杂志,2016,19(7):821-822.
[10]郑榕英,张爱龙,肖雪明,等.乳腺癌易感基因1、E-钙黏蛋白、p120在乳腺浸润性导管癌组织的表达及其临床意义[J].中华实验外科杂志,2017,34(11):1950-1953.
[11]黄建强,常瑞明,温立强,等.E-cadherin在肝癌组织中的表达及其对肝癌患者预后的影响[J].第三军医大学学报,2014,36(22):2301-2303.
[12]曾思恩,肖胜军,张小玲,等.肝细胞肝癌中转录辅抑制因子CtB P1与其靶基因E-cadherin的表达[J].临床与实验病理学杂志,2010,26(6):655-657.
[2]刘妍,徐勇,高超,等.shR NA沉默CtB P2基因对前列腺癌细胞的增殖作用[J].天津医药,2014,42(10):977-980.
[3]朱理辉,罗勇,廖文秋,等.MicroR NA-219-5p靶向E-钙黏蛋白调控上皮间质转化抑制肝癌细胞侵袭转移[J].中国现代医学杂志,2016,26(18):22-29.
[4]胡洁,边立会,王晓玉,等.胆管细胞癌中CtB P1、Zeb1、Zeb2与E-cadherin蛋白的表达及临床意义[J].临床与实验病理学杂志,2017,33(4):365-369.
[5]陈伟.全基因组和外显子组测序在肝癌研究中的应用进展[J].中华肝胆外科杂志,2017,23(11):784-789.
[6]李运千,黄承信,曾思恩,等.羧基末端结合蛋白1对肝癌细胞HepG 2增殖的影响[J].肿瘤防治研究,2014,41(5):384-387.
[7]李霞,葛爱敏.miR-617在宫颈癌组织中的表达及其对宫颈癌SiHa细胞生物学行为的影响[J].现代妇产科进展,2016,25(8):586-588.
[8]常磊,袁玉峰,郭涛,等.波形蛋白、E-钙黏蛋白在原发性肝癌上皮间质转化过程中的表达及其意义[J].中华肝胆外科杂志,2015,21(1):9-13.
[9]王红山,刘迎,解寒冰,等.EphA2和上皮型钙黏蛋白表达与胃癌侵袭转移的关系[J].中华胃肠外科杂志,2016,19(7):821-822.
[10]郑榕英,张爱龙,肖雪明,等.乳腺癌易感基因1、E-钙黏蛋白、p120在乳腺浸润性导管癌组织的表达及其临床意义[J].中华实验外科杂志,2017,34(11):1950-1953.
[11]黄建强,常瑞明,温立强,等.E-cadherin在肝癌组织中的表达及其对肝癌患者预后的影响[J].第三军医大学学报,2014,36(22):2301-2303.
[12]曾思恩,肖胜军,张小玲,等.肝细胞肝癌中转录辅抑制因子CtB P1与其靶基因E-cadherin的表达[J].临床与实验病理学杂志,2010,26(6):655-657.