HPLC法测定小儿消食片中槟榔碱的含量
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摘要
目的建立高效液相色谱法测定小儿消食片中槟榔碱含量的方法。方法采用HPLC对槟榔碱进行含量测定,色谱柱为Thermo BioBasic SCX(250×4.6mm,5μm),流动相为乙腈-0.1%磷酸溶液(用氨水调pH值3.80)(65∶35),检测波长为215nm,流速为1mL·min-1,柱温为25℃。结果氢溴酸槟榔碱在2.99～47.84μg·mL~(-1)范围内呈良好的线性关系,r=0.9999,平均回收率为97.03%,RSD为1.16%。结论该方法操作简便,专属性强,重复性好,可作为小儿消食片中槟榔碱的含量测定方法。
OBJECTIVE To establish a method for the determination of arecoline in pediatric digestion tablets with HPLC method.METHODS The content of arecoline was determined by HPLC,the column was Thermo BioBasic SCX(250×4.6mm,5μm),the mobile phase was acetonitrile-0.1% phosphoric acid solution(to adjust the pH with ammonia to 3.80)(65∶35),the detection wavelength was 215 nm,the flow rate was 1mL·min-1 and the column temperature was 25℃.RESULTS The good linearity of hydrochloric acid iscoline was in the range of 2.99～47.84μg·mL~(-1)(r=0.9999),the average recovery was 97.03% and the RSD was 1.16%.CONCLUSION The method was simple,specificity and with good reproducible.It can be used for the determination of arecoline in pediatric digestion tablets.
OBJECTIVE To establish a method for the determination of arecoline in pediatric digestion tablets with HPLC method.METHODS The content of arecoline was determined by HPLC,the column was Thermo BioBasic SCX(250×4.6mm,5μm),the mobile phase was acetonitrile-0.1% phosphoric acid solution(to adjust the pH with ammonia to 3.80)(65∶35),the detection wavelength was 215 nm,the flow rate was 1mL·min-1 and the column temperature was 25℃.RESULTS The good linearity of hydrochloric acid iscoline was in the range of 2.99～47.84μg·mL~(-1)(r=0.9999),the average recovery was 97.03% and the RSD was 1.16%.CONCLUSION The method was simple,specificity and with good reproducible.It can be used for the determination of arecoline in pediatric digestion tablets.
引文
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[17]周绪正,张继瑜,李金善,等.氢澳酸槟榔碱安全性评价[J].湖北农业科学,2010,49(9):2268-2270.
[2]李晨,胡兵,吕涛,等.槟榔对豚鼠胃平滑肌的作用及机制探讨[J].中医学报,2011,12(26):1477-1479.
[3]倪依东,王建华,王汝俊.槟榔水提液对胃肠运动的影响[J].中药药理与临床,2003,19(5):27-29.
[4]M Am,K Ah,P Gs.Chemistry and pharmaeolugy of betel nut areea catechu linu[J].Int J Pathol Clinic Med,2010,30(2):69-69.
[5]刘东林,王小莹,杨冰,等.槟榔药理毒理研究进展[J].中国中药杂志,2013,38(14):2273-2275.
[6]李晨,魏睦新.氢溴酸槟榔碱对豚鼠体外胃不同部位肌条作用及其机制[J].中国中西医结合消化杂志,2007,2(77):77-80.
[7]李习雄,胡冠英,张三印.槟榔毒性机制的研究进展[J].中国实验方剂学杂志,2015,21(19):212-216.
[8]C Gh,N Ga,C Le,et al.M1 receptor activation is a requirement for arecoline analgesia[J].Farmaco,2001,56(5-7):383-385.
[9]吕俊辰,弓宝,孙佩文.槟榔药用和食用安全性研究概况[J].中草药,2017,48(2):384-390.
[10]曾薇,古桂花,李建新,等.槟榔碱的肾毒性实验研究[J].湖南中医药大学学报,2015,35(6):6-8.
[11]古桂花,曾薇,胡虹,等.槟榔粗提物及槟榔碱对小鼠肝细胞凋亡的影响[J].中药药理与临床,2013,29(2):56-59.
[12]杨华,郭元满,谭泽云,等.HPLC法同时测定四磨汤滴丸中辛弗林、槟榔碱和去甲异波尔定的含量[J].中医药导报,2016,22(2):40-43.
[13]汤丽娟,吴皓,郁红礼.HPLC同时测定大腹皮中4种生物碱含量[J].中华中医药杂志,2014,29(11):3583-3586.
[14]毛春芹,陆兔林,季德,等.高效液相色谱法测定不同产地大腹皮中槟榔碱的含量[J].中国药学杂志,2013,48(6):909-911.
[15]肖冰梅,肖嫩群,彭买姣,等.槟榔碱提神效果及急性毒性作用研究[J].中国当代医药,2013,20(20):14-16.
[16]赵成莹,张继瑜,周绪正,等.槟榔碱、氢溴酸槟榔碱的急性毒性研究[J].中兽医医药杂志,2007,26(1):33-34.
[17]周绪正,张继瑜,李金善,等.氢澳酸槟榔碱安全性评价[J].湖北农业科学,2010,49(9):2268-2270.