PI3K信号通路中LCK、LAT、SYK、PKC基因在特发性眼眶炎性假瘤中差异表达分析
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摘要
目的分析特发性眼眶炎性假瘤患者与单纯眼眶海绵状血管瘤患者病变泪腺标本组织中LCK、LAT、SYK、PKC的差异表达情况,为特发性眼眶炎性假瘤发病机理提供参考。方法采用全基因组表达谱芯片技术检测特发性眼眶炎性假瘤患者病变泪腺组织与单纯眼眶海绵状血管瘤患者瘤体组织中信号通路的差异性基因表达。采用PCR扩增技术,免疫组织化学染色方法及Western blot法检测特发性眼眶炎性假瘤患者病变泪腺组织中LCK、LAT、SYK、PKC的mRNA及蛋白质表达情况,并以单纯眼眶海绵状血管瘤患者瘤体组织标本作对照。结果经基因芯片检测分析,实验组与对照组相比较,PI3K信号通路(PI3Ksignaling pathway)表达显著上调,其中LCK、LAT、SYK、PKC 4个基因差异性表达最为明显(P<0.05)。经PCR扩增技术检测、免疫组织化学方法及Western blot检测,IOIP组LCK、LAT、SYK、PKC的mRNA及蛋白表达均呈阳性,眼眶海绵状血管瘤组表达均呈阴性。结论PI3K信号通路中LCK、LAT、SYK、PKC因子高表达在眼眶特发性炎性假瘤的发生发展中起重要作用。
Objective To analyze the differential expression of LCK、LAT、SYK and PKC in lacrimal gland specimens of patients with idiopathic orbital inflammatory pseudotumor or simple orbital cavernous hemangioma,and provide reference for the pathogenesis of idiopathic orbital inflammatory pseudotumor.Methods Different expression of pathways on patients with Idiopathic Orbital Inflammatory Pseudotumor and orbital cavernous hemangioma were detected by gene chips.The expression of LCK、LAT、SYK、PKC in level of mRNA and protein were detected by PCR,immune histochemical exam and western blot in Idiopathic Orbital Inflammatory Pseudotumor and orbital cavernous hemangioma.Orbital cavernous hemangioma was considered as control group.Results Compared with control group,the experimental group was found that the expression of PI3 Ksignaling pathway increased and there were LCK、LAT、SYK、PKC expressed differently.Stripes of DNA amplification were founded in LCK、LAT、SYK、PKC while no stripe was showed in RASA1 in Idiopathic Orbital Inflammatory Pseudotumor.There was no stripe of DNA amplification in orbital cavernous hemangioma.LCK、LAT、SYK、PKC were positive expression in Idiopathic Orbital Inflammatory Pseudotumor.The expression of LCK、LAT、SYK、PKC was normal in orbital cavernous hemangioma.Positive stripes were showed in LCK、LAT、SYK、PKC Idiopathic Orbital Inflammatory Pseudotumor.There was no stripe in orbital cavernous hemangioma.Conclusion Over-expression of LCK、LAT、SYK、PKC in PI3 K signaling pathwaymay take part in the pathogensis of Idiopathic Orbital Inflammatory Pseudotumor.
Objective To analyze the differential expression of LCK、LAT、SYK and PKC in lacrimal gland specimens of patients with idiopathic orbital inflammatory pseudotumor or simple orbital cavernous hemangioma,and provide reference for the pathogenesis of idiopathic orbital inflammatory pseudotumor.Methods Different expression of pathways on patients with Idiopathic Orbital Inflammatory Pseudotumor and orbital cavernous hemangioma were detected by gene chips.The expression of LCK、LAT、SYK、PKC in level of mRNA and protein were detected by PCR,immune histochemical exam and western blot in Idiopathic Orbital Inflammatory Pseudotumor and orbital cavernous hemangioma.Orbital cavernous hemangioma was considered as control group.Results Compared with control group,the experimental group was found that the expression of PI3 Ksignaling pathway increased and there were LCK、LAT、SYK、PKC expressed differently.Stripes of DNA amplification were founded in LCK、LAT、SYK、PKC while no stripe was showed in RASA1 in Idiopathic Orbital Inflammatory Pseudotumor.There was no stripe of DNA amplification in orbital cavernous hemangioma.LCK、LAT、SYK、PKC were positive expression in Idiopathic Orbital Inflammatory Pseudotumor.The expression of LCK、LAT、SYK、PKC was normal in orbital cavernous hemangioma.Positive stripes were showed in LCK、LAT、SYK、PKC Idiopathic Orbital Inflammatory Pseudotumor.There was no stripe in orbital cavernous hemangioma.Conclusion Over-expression of LCK、LAT、SYK、PKC in PI3 K signaling pathwaymay take part in the pathogensis of Idiopathic Orbital Inflammatory Pseudotumor.
引文
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[6]马建民,李金茹,葛心.特发性眼眶炎性假瘤患者血清中IgG其亚型水平的研究[J].临床眼科杂志,2015,5(2):105-107.
[7]Hrtmann W,Digon-Sontgerath B,Koch A,et al.Phosphatidylinositol 3′-kinase/AKT signaling is activated immedulloblastoma cell proliferation and is associated with reduced expression of PTEN[J].Clinical cancer research,2016,12(10):3019-3027.
[8]Or Y,Hui AB,Tam K,et al.Characterization of chromosome 3q and 12q amplicons in nasopharyngeal carcinoma cell lines[J].International journal of oncology,2010,26(1):9-56.
[9]Nyakern M,Tazzari PL,Finell IC,et al.Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral Bloodmon onuclear cells from high-risk myelodysplastic syndrome patients[J].Leukemia,2006,20(2):230-238.
[10]王萍,李养军,张少波,等.大剂量甲强龙联合小剂量甲氨蝶呤治疗特发性眼眶炎性假瘤疗效分析[J].眼科新进展,2015,35(10):977-979.
[2]李静,马建民.特发性眼眶炎性假瘤病因及发病机制的研究[J].中华实验眼科杂志,2012,5(30):471-475.
[3]Swamy BN,Mccluskey P,Nemet A,et al.Idiopathic orbital inflammatory syndrome:Clinical features and treatment outcomes[J].British Journal of Ophthalmology,2007,91(12):1667-1670.
[4]Joshi AK,Desai W,Patil S,et al.A rare case of idiopathic orbital inflammatory syndrome[J].2015,8(6):792.
[5]Zou Y,Ge M,Wang X.Targeting PI3K-AKT-mTOR by LY3023414inhibits human skin squamous cell carcinoma cell growth in vitro and in vivo[J].Biochemical&Biophysical Research Communications,2017,490(2):385-392.
[6]马建民,李金茹,葛心.特发性眼眶炎性假瘤患者血清中IgG其亚型水平的研究[J].临床眼科杂志,2015,5(2):105-107.
[7]Hrtmann W,Digon-Sontgerath B,Koch A,et al.Phosphatidylinositol 3′-kinase/AKT signaling is activated immedulloblastoma cell proliferation and is associated with reduced expression of PTEN[J].Clinical cancer research,2016,12(10):3019-3027.
[8]Or Y,Hui AB,Tam K,et al.Characterization of chromosome 3q and 12q amplicons in nasopharyngeal carcinoma cell lines[J].International journal of oncology,2010,26(1):9-56.
[9]Nyakern M,Tazzari PL,Finell IC,et al.Frequent elevation of Akt kinase phosphorylation in blood marrow and peripheral Bloodmon onuclear cells from high-risk myelodysplastic syndrome patients[J].Leukemia,2006,20(2):230-238.
[10]王萍,李养军,张少波,等.大剂量甲强龙联合小剂量甲氨蝶呤治疗特发性眼眶炎性假瘤疗效分析[J].眼科新进展,2015,35(10):977-979.