转FGF5s基因的陕北白绒山羊粪便菌群分析
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摘要
为了对转FGF5s基因陕北白绒山羊的生物安全进行评价,试验以转FGF5s基因和野生型陕北白绒山羊为研究对象,采集其新鲜粪便样品,用灭菌生理盐水进行梯度稀释后分别接种LB固体培养基、胆硫乳琼脂培养基、肠球菌琼脂培养基、伊红美蓝琼脂培养基和甘露醇盐琼脂培养基,37℃恒温过夜培养后分别对培养基上生长的所有需氧菌、沙门氏杆菌、肠球菌、大肠菌群和金黄色葡萄球菌进行菌落计数,并进行差异显著性检验。结果表明:微生物在5种培养基上形成不同的菌落形态,菌落计数之后对数据进行统计分析,结果差异不显著(P>0.05)。说明转FGF5s基因与野生型陕北白绒山羊粪便中的需氧菌菌群结构间不存在显著差异。
引文
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[2]闫昱,屈雷.陕北白绒山羊产业发展现状和策略研究[J].榆林学院学报,2008,18(2):10-15.
[3]李华玮,郑鸣.转基因技术发展现状及其对社会发展的影响[J].黑龙江畜牧兽医,2014(11上):229-230.
[4]OTA Y,SAITOH Y,SUZUKIi S,et al.Fibroblast growth factor 5 inhibits hair growth by blocking dermal papilla cell activation[J].Biochem Biophys Res Commun,2002,290:169-176.
[5]何晓琳,袁超,屈雷,等.Piggy Bac转座子介导的转FGF5s基因绒山羊胎儿成纤维细胞的制备[J].畜牧兽医学报,2013,44(12):1926-1931.
[6]LAPRRA J M,SANZ Y.Interactions of gut microbiota with functional food components and nutraceuticals[J].Pharmacol Res,2010,61(3):219-225.
[7]OJETTI V,GIGANTEM G,AINORA M E,et al.Microflora imbalance and gastrointestinal diseases[J].Digest Liver Dis,2009,3(2):35-39.
[8]ROUND J L,MAZMANIAN S K.The gut microbiota shapes intestinal immune responses during health and disease[J].Nat Rev Immunol,2009,9(5):313-323.
[9]TANG M,ZHENG X,CHENG W,et al.Safety assessment of s Fat-1 transgenic pigs by detecting their co-habitant microbe in intestinal tract[J].Transgenic Res,2011,20:749-758.
[10]SCHRODER M,POULSEN M,WILCKS A,et al.A 90-day safety study of geneticallymodified rice expressing Cry1Ab protein(Bacillus thuringiensis toxin)in Wistar rats[J].Food Chem Toxicol,2007,45:339-349.
[11]POULSEN M,KROGHSBO S,SCHRODER M,et al.A 90-day safety study in Wistar rats fed genetically modified rice expressing snowdrop lectin Galanthus nivalis(GNA)[J].Food Chem Toxicol,2007,45:350-363.
[12]禹慧明,廖玲,陈平洁,等.断奶仔猪肠道菌群的研究[J].中国微生态杂志,2000,4(12):81-82.
[13]高辉.转PEPCK-1基因猪的生物安全评价[D].合肥:安徽农业大学,2013.
[14]余乾.转e GFP基因小鼠环境释放安全性评价研究[D].重庆:西南大学,2014.
[15]吴洁云.h LYZ转基因山羊粪便对于土壤微生物群落及基因漂移的影响[D].杨凌:西北农林科技大学,2012.