摘要
以唐菖蒲‘超级红’品种为试验材料,采用RT-PCR技术克隆得到其PYL1基因并对其生物信息学进行分析,进一步研究ABA浸泡种球处理后球茎发芽率与PYL1基因表达水平之间的相关性。结果表明:该基因具有687 bp的开放阅读框(ORF),编码228个氨基酸,相对分子质量为25.02 kD,等电点为5.35,具有ABA结合域;其cDNA全长为838 bp,命名为GhPYL1。系统发育树分析表明,GhPYL1与油棕、芭蕉等植物的PYL序列相似性较高。随着ABA处理浓度的逐渐增加,唐菖蒲球茎发芽率逐渐降低,而GhPYL1基因的表达量逐渐升高。球茎发芽率与GhPYL1基因表达水平呈负相关关系,表明该基因可能参与ABA调控的唐菖蒲球茎休眠过程。
Using Gladiolus hybridus 'Su preme Red' variety as the experimental material, the PYL1 gene was cloned by RT-PCR technique and the bioinformatics analysis was carried out. The correlation between corms germination rate and PYL1 gene expression level after ABA soaking corm treatment was further studied. The results showed that the open reading frame(ORF) of this gene was 687 bp, encoding 228 amino acids with the relative molecular mass of 25.02 kD and the isoelectric point of 5.35 with an ABA binding domain. The full length of cDNA was 838 bp, named GhPYL1. Phylogenetic tree analysis showed that GhPYL1 had higher similarity to PYL sequences of plants such as oil palm and plantain. With gradual increase of ABA treatment concentration, the germination rate of gladiolus corms decreased gradually, while the expression of GhPYL1 gene gradually increased. There was a negative correlation between the germination rate of corms and the expression level of GhPYL1 gene, indicating that this gene might be involved in the dormancy process of gladiolus corms regulated by ABA.
引文
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