辣木叶的UPLC特征指纹图谱研究
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摘要
建立辣木叶的超高效液相(UPLC)特征指纹图谱分析方法,为辣木叶质量控制提供科学依据。采用UPLC建立辣木叶的特征指纹图谱,色谱柱为Agilent Eclipse XDB-C18(3. 0 mm×100 mm,1. 8μm),流动相为乙腈-0. 01%三氟乙酸水溶液,梯度洗脱,流速为0. 5 m L·min-1,检测波长为210 nm,柱温为35℃。采用"中药色谱指纹图谱相似度评价系统2004A版"对14批辣木叶样品进行相似度分析以比较各个批次样品间的一致性。建立了辣木叶的UPLC特征指纹图谱,确定了12个共有峰,并根据对照品和UPLC-MS对这12个色谱峰进行结构确认,其相对保留时间为0. 08 (峰1,腺苷),0. 14 (峰2,L-苯丙氨酸),0. 22 (峰3,5-咖啡酰奎宁酸),0. 28 (峰4,L-色氨酸),0. 42 (峰5,4-咖啡酰奎宁酸),0. 65 (峰6,维采宁-2),0. 94 (峰7,牡荆素),0. 96 (峰8,异牡荆素),1. 00 (峰9,异槲皮素),1. 11 [峰10,槲皮素3-O-(6″-丙二酰基)-β-D-葡萄糖苷],1. 21(峰11,紫云英苷),1. 37 [峰12,山柰酚3-O-(6″-丙二酰基)-β-D-葡萄糖苷]。该方法首次建立了辣木叶的UPLC特征指纹图谱,方法简便、快速、准确性高、重复性好,可为辣木叶的质量评价和控制提供科学依据。
This study aims to establish the characteristic fingerprint of the leaves of Moringa oleifera by Ultra High Performance Liquid Chromatography(UPLC) for its quality control. The method was developed on a column of Agilent Eclipse XDB-C18 with acetonitrile-0. 01% TFA solution as the mobile phase by gradient elution at a flow rate of 0. 5 m L·min-1. The detective wavelength was 210 nm,and the column temperature was 35 ℃. The 14 batches of the leaves of M. oleifera were compared for the similarity by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004 A). The UPLC characteristic fingerprint was established,and twelve common peaks were identified by comparison with the references and UPLC-MS. The relative retention times were 0. 08(No. 1,adenosine),0. 14(No. 2,L-phenylalanine),0. 22(No. 3,5-caffeoylquinic acid),0. 28(No. 4,L-tryptophane),0. 42(No. 5,4-caffeoylquinic acid),0. 65(No. 6,vicenin-2),0. 94(No. 7,vitexin),0. 96(No. 8,isovitexin),1. 00(No. 9,isoquercitrin),1. 11 [No. 10,quercetin 3-O-β-D-(6″-malonyl)-glucopyranoside],1. 21(No. 11,astragalin) and 1. 37[No. 12,kaempferol 3-O-β-D-(6″-malonyl)-glucopyranoside]. It is the first time to establish the UPLC characteristic fingerprint of the leaves of M. oleifera. The method is simple,quick and reproducible with high precision,which can provide a scientific basis for the quality control of the leaves of M. oleifera.
This study aims to establish the characteristic fingerprint of the leaves of Moringa oleifera by Ultra High Performance Liquid Chromatography(UPLC) for its quality control. The method was developed on a column of Agilent Eclipse XDB-C18 with acetonitrile-0. 01% TFA solution as the mobile phase by gradient elution at a flow rate of 0. 5 m L·min-1. The detective wavelength was 210 nm,and the column temperature was 35 ℃. The 14 batches of the leaves of M. oleifera were compared for the similarity by using Traditional Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System(2004 A). The UPLC characteristic fingerprint was established,and twelve common peaks were identified by comparison with the references and UPLC-MS. The relative retention times were 0. 08(No. 1,adenosine),0. 14(No. 2,L-phenylalanine),0. 22(No. 3,5-caffeoylquinic acid),0. 28(No. 4,L-tryptophane),0. 42(No. 5,4-caffeoylquinic acid),0. 65(No. 6,vicenin-2),0. 94(No. 7,vitexin),0. 96(No. 8,isovitexin),1. 00(No. 9,isoquercitrin),1. 11 [No. 10,quercetin 3-O-β-D-(6″-malonyl)-glucopyranoside],1. 21(No. 11,astragalin) and 1. 37[No. 12,kaempferol 3-O-β-D-(6″-malonyl)-glucopyranoside]. It is the first time to establish the UPLC characteristic fingerprint of the leaves of M. oleifera. The method is simple,quick and reproducible with high precision,which can provide a scientific basis for the quality control of the leaves of M. oleifera.
引文
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[3] Fombang E N,Saa R W. Antihyperglycemic activity of Moringa oleifera Lam leaf functional tea in rat models and human subjects[J]. Food Nutr Sci,2016,7(11):1021.
[4] Chen K H,Chen Y J,Yang C H,et al. Attenuation of the extract from Moringa oleifera on monocrotaline-induced pulmonary hypertension in rats[J]. Chin J Physiol,2012,55(1):22.
[5] Ghasi S,Nwobodo E,Ofili J O. Hypocholesterolemic effects of crude extract of leaf of Moringa oleifera Lam in high-fat diet fed wistar rats[J]. J Ethnopharmacol,2000,69(1):21.
[6] Etim E I,Umoh E E,Okokon J E,et al. Hepatoprotective activity of the methanol leaf extract of moringa oleifera(Lam)against carbontetrachloride(CCI4)induced liver toxicity in rats[J].World J Pharm Pharm Sci,2016,5(6):143.
[7] Vongsak B,Gritsanapan W,Wongkrajang Y,et al. In vitro inhibitory effects of Moringa oleifera leaf extract and its major components on chemiluminescence and chemotactic activity of phagocytes[J]. Nat Prod Comm,2013,8(11):1559.
[8] Hossain A,Khatun A,Munshi M K,et al. Study on antibacterial and antioxidant activities of raw and fermented Moringa oleifera Lam. leaves[J]. J Microbiol Biot Res,2016,6(4):23.
[9] Choudhary M K,Bodakhe S H,Gupta S K. Assessment of the antiulcer potential of Moringa oleifera root-bark extract in rats[J]. J Acupunct Meridian Stud,2013,6(4):214.
[10]孙一蕊,范春林,王英,等.观辣树的化学成分研究进展[J].中国药房,2012,23(31):2965.
[11]金玲,娄涛涛,刘旋,等.辣木叶的HPLC指纹图谱[J].中国实验方剂学杂志,2017,22(8):86.
[12]李锋华,王洪庆,苏现明,等.辣木叶正丁醇部位化学成分研究[J].中国中药杂志,2018,43(1):114.
[13]熊山,陈玉武,叶祖光.楮实子的化学成分研究[J].现代药物与临床,2009,24(1):34.
[14]刘珊珊,周兴清,梁彩霞,等.吴茱萸水提取物化学成分研究[J].中国实验方剂学杂志,2016,22(8):58.
[15]夏鹏飞,宋爽,冯子明,等.掌叶苹婆化学成分的研究[J].中国中药杂志,2009,34(20):2604.
[16]纳智,许又凯.版纳藤黄化学成分的研究[J].中国中药杂志,2009,34(18):2338.
[17]李雨蔚,李荣娇,袁绿益,等.西藏秦艽花的化学成分研究[J].中草药,2015,46(14):2052.
[18] Jaramillo K,Dawid C,Hofmann T,et al. Identification of antioxidative flavonols and anthocyanins in Sicana odorifera fruit peel[J]. J Agric Food Chem,2011,59(3):975.
[19]刘安,徐丽珍,邹忠梅,等.对叶豆黄酮类化学成分研究[J].中国中药杂志,2009,34(7):861.
[20]李玉林,廖志新,孙洪发,等.急弯棘豆化学成分的研究[J].中草药,1998(3):149.