针刺下关穴对过敏性鼻炎大鼠血清IL-6、TNF-α表达的影响
|
摘要
目的:探讨针刺下关穴对过敏性鼻炎(AR)大鼠血清白介素-6(IL-6)和肿瘤坏死因子-α(TNF-α)表达的影响。方法:选用健康SD大鼠,分为正常组、模型组、阳性组和针灸组,建立AR大鼠模型,对针灸组大鼠采用针刺下关穴进行治疗,阳性组大鼠予丙酸氟替卡松喷鼻治疗,均每天1次,共治疗7d。对大鼠过敏症状进行评分;心脏取血分离血清采用酶联免疫法检测IL-6及TNF-α的水平。结果:与模型组比较,针灸组过敏症状评分明显降低(P <0. 01),血清IL-6水平及TNF-α的水平显著下降,差异均有统计学意义(P <0. 01,P <0. 05);针灸组与阳性组各项指标比较,差异无统计学意义(P>0. 05)。结论:针刺下关穴能有效治疗过敏性鼻炎,其机制可能与其抑制IL-6和TNF-α的活性有关。
Objective: To investigate the effect of acupuncture at Xiaguan point on serum levels of interleukin-6( IL-6) and tumor necrosis factor-α( TNF-α) in rats with allergic rhinitis( AR). Methods: Healthy Sprague-Dawley rats were divided into normal group,model group,positive group,and acupuncture group. A rat model of AR was established. The rats in the acupuncture group were given acupuncture at Xiaguan point,and those in the positive group were given nasal spray of fluticasone propionate,once a day for 7 days. The allergic symptom score was determined,and blood samples were collected from the heart to measure the serum levels of IL-6 and TNF-α by ELISA. Results: Compared with the model group,the acupuncture group had significantly lower allergic symptom score( P < 0. 01) and levels of IL-6 and TNF-α( P < 0. 01 or P < 0. 05). There were no significant differences in these indices between the acupuncture group and the positive group( P > 0. 05). Conclusion: Acupuncture at Xiaguan point has a good clinical effect in the treatment of AR,possibly by inhibiting the activity of IL-6 and TNF-α.
Objective: To investigate the effect of acupuncture at Xiaguan point on serum levels of interleukin-6( IL-6) and tumor necrosis factor-α( TNF-α) in rats with allergic rhinitis( AR). Methods: Healthy Sprague-Dawley rats were divided into normal group,model group,positive group,and acupuncture group. A rat model of AR was established. The rats in the acupuncture group were given acupuncture at Xiaguan point,and those in the positive group were given nasal spray of fluticasone propionate,once a day for 7 days. The allergic symptom score was determined,and blood samples were collected from the heart to measure the serum levels of IL-6 and TNF-α by ELISA. Results: Compared with the model group,the acupuncture group had significantly lower allergic symptom score( P < 0. 01) and levels of IL-6 and TNF-α( P < 0. 01 or P < 0. 05). There were no significant differences in these indices between the acupuncture group and the positive group( P > 0. 05). Conclusion: Acupuncture at Xiaguan point has a good clinical effect in the treatment of AR,possibly by inhibiting the activity of IL-6 and TNF-α.
引文
[1]马云翔,刘敏.针刺蝶腭神经节配合艾灸治疗变应性鼻炎45例报告[J].中国实用医药,2013,8(9):222.
[2]田芳洁,李雪盛,李进让,等.北京地区变应性鼻炎的吸入性变应原调查[J].空军医学杂志,2015,31(2):109.
[3]陈思洁,吴飞虎.过敏性鼻炎的中医外治法研究进展[J].中医药临床杂志,2013,22(5):469-470.
[4]张丽文.下关穴为主针刺治疗过敏性鼻炎57例[J].临床医学研究与实践,2016,1(8):62.
[5]张仲林,钟玲,凌保东,等.玉屏风散调控变应性鼻炎大鼠IL-6、TNF-α活性的实验研究[J].中成药,2014,36(9):1804-1808.
[6]陆倩.变应性鼻炎大鼠翼腭神经节内VIP、NOS阳性神经元的变化[D].南京:南京医科大学,2006.
[7]郑先丽,田永萍,雒海燕,等.温通针法对过敏性鼻炎大鼠血清免疫球蛋白E、白介素-1β、肿瘤坏死因子-α含量的影响[J].针刺研究,2018,43(1):34-37.
[8]孔乔,李安,吕萌,等.过敏性鼻炎的预防与中医外治法研究进展[J].内蒙古中医药,2017,36(12):142-143.
[9]陈泽莉,冯祯根,陈荷光.下关穴双针电针深刺治疗过敏性鼻炎的临床研究[C]//浙江省针灸学会2015学术年会论文汇编,2015:35-37.
[2]田芳洁,李雪盛,李进让,等.北京地区变应性鼻炎的吸入性变应原调查[J].空军医学杂志,2015,31(2):109.
[3]陈思洁,吴飞虎.过敏性鼻炎的中医外治法研究进展[J].中医药临床杂志,2013,22(5):469-470.
[4]张丽文.下关穴为主针刺治疗过敏性鼻炎57例[J].临床医学研究与实践,2016,1(8):62.
[5]张仲林,钟玲,凌保东,等.玉屏风散调控变应性鼻炎大鼠IL-6、TNF-α活性的实验研究[J].中成药,2014,36(9):1804-1808.
[6]陆倩.变应性鼻炎大鼠翼腭神经节内VIP、NOS阳性神经元的变化[D].南京:南京医科大学,2006.
[7]郑先丽,田永萍,雒海燕,等.温通针法对过敏性鼻炎大鼠血清免疫球蛋白E、白介素-1β、肿瘤坏死因子-α含量的影响[J].针刺研究,2018,43(1):34-37.
[8]孔乔,李安,吕萌,等.过敏性鼻炎的预防与中医外治法研究进展[J].内蒙古中医药,2017,36(12):142-143.
[9]陈泽莉,冯祯根,陈荷光.下关穴双针电针深刺治疗过敏性鼻炎的临床研究[C]//浙江省针灸学会2015学术年会论文汇编,2015:35-37.