葡萄籽原花青素对肥胖大鼠肠粘膜的影响
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摘要
为探究葡萄籽原花青素(GSP)对营养肥胖模型大鼠肠粘膜通透性的影响,本试验设置了正常、模型、低剂量、中剂量及高剂量5个处理组,其中正常组投喂普通饲料,灌胃3 m L蒸馏水;模型组和低、中、高剂量组选用32只高脂饲料诱导的肥胖大鼠,模型组灌胃蒸馏水,低、中、高剂量组分别灌胃75、162.5、250 mg·kg-1BW·d-1GSP,灌胃处理6周再禁食12 h后处死,经解剖后测定各处理组大鼠的生理生化相关指标。结果表明,高脂饲料造模成功率48.75%;与模型组相比,低、中、高剂量组大鼠D-乳酸、乳果糖/甘露醇排出率显著降低,回肠绒毛高度/隐窝深度、紧密连接蛋白Occludin和ZO-1表达量显著升高;高剂量组大鼠的回肠杯状细胞数显著升高。综上,葡萄籽原花青素可以改善肥胖应激导致的大鼠肠道粘膜屏障损伤,这在一定程度上揭示了葡萄籽原花青素减肥降脂的作用机理。
To investigate the effects of grape seed procyanidins( GSP) on the intestinal mucosal permeability in nutritional obesity rat models. Normal diet fed rats were set as standard groups,which were given 3 mL distilled water 32 high-fat diet-induced obesity rats were divided into 4 groups: control group( distilled water),low dose group,[GSP,75 mg·kg-1·BW·d-1],middle dose group,[GSP,162.5 mg·kg-1·BW·d-1],high dose group,[GSP,250 mg·kg-1·BW·d-1]. All groups intragastric administration for six weeks,and stop feeding about 12 hours. Then the rats were executed,and the relevant indexes of each group were determined. The results showed that the successful rate of establishing the high-fat diet rat models was 48.75%. Compared with model group,the excretion rates of D-lactic acid and lactulose/mannitol in the GSP dose group were significantly lower; the ileal villi height/crypt depth,tight junction protein Occludin and ZO-1 expression were significantly increased,and the number of ileal goblet cells in the high-dose group was significantly higher than that in the control group. This study shows that GSP can improve the intestinal mucosal barrier injury induced by obesity stress in rats,which explained the anti-obesity effect and mechanism of GSP on the obese rats induced by high fat diet.
To investigate the effects of grape seed procyanidins( GSP) on the intestinal mucosal permeability in nutritional obesity rat models. Normal diet fed rats were set as standard groups,which were given 3 mL distilled water 32 high-fat diet-induced obesity rats were divided into 4 groups: control group( distilled water),low dose group,[GSP,75 mg·kg-1·BW·d-1],middle dose group,[GSP,162.5 mg·kg-1·BW·d-1],high dose group,[GSP,250 mg·kg-1·BW·d-1]. All groups intragastric administration for six weeks,and stop feeding about 12 hours. Then the rats were executed,and the relevant indexes of each group were determined. The results showed that the successful rate of establishing the high-fat diet rat models was 48.75%. Compared with model group,the excretion rates of D-lactic acid and lactulose/mannitol in the GSP dose group were significantly lower; the ileal villi height/crypt depth,tight junction protein Occludin and ZO-1 expression were significantly increased,and the number of ileal goblet cells in the high-dose group was significantly higher than that in the control group. This study shows that GSP can improve the intestinal mucosal barrier injury induced by obesity stress in rats,which explained the anti-obesity effect and mechanism of GSP on the obese rats induced by high fat diet.
引文
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[8] Kamel C,Mohamed M,Takwa B,Khawla J,Veronique E M M,Ferid L,Ezzedine A. Safety evaluation,anti-oxidative and antiinflammatory effects of subchronically dietary supplemented high dosing grape seed powder(GSP)to healthy rat[J]. Biomedicine&Pharmacotherapy,2018,107:534-546
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[18]张媛,杨晨玉,韩亚楠,陈耀星,王子旭,曹静,董玉兰. 5-羟色胺对断奶腹泻仔鼠小肠组织学结构的影响[J].中国农业大学学报,2017,22(2):80-86
[19]甘元涛,赵少勇.肠黏膜屏障损伤与紧密连接蛋白ZO-1的关系研究进展[J].现代医药卫生,2016,32(7):1027-1029
[20]邓宸玺,王自蕊,游金明,瞿明仁,叶亚玲,辛向荣,潘珂.丙氨酰-谷氨酰胺二肽对仔猪小肠上皮细胞间紧密连接蛋白occludin定位与表达的影响[J].动物营养学报,2014,26(3):694-700
[21]秘迎君.中国九省成人超重、肥胖的患病率及其与全死因死亡风险的关联研究[D].石家庄:河北医科大学,2017
[22]龚芳,艾宇航,黄绍华,贵春梅,陈超.连续性血液净化对重症胰腺炎患者炎症因子、内毒素及肠道黏膜屏障功能的影响[J].现代生物医学进展,2017,17(10):1849-1851,1895
[23]王晓枫.抑制肠源性内毒素改善大鼠极限肝切除术预后的三重作用及机制[D].重庆:中国人民解放军陆军军医大学,2013
[24]董莉君.高脂饮食对不同年龄小鼠脂肪组织炎症的影响[D].新乡:新乡医学院,2016
[25] Berg R D. Bacterial translocation from the gastrointestinal tract[J].Trends in Microbiology,1995,3(4):149-154
[26]刘芳.从炎症角度解析中度高脂饮食诱导肥胖对内分泌代谢及骨骼肌功能的影响[D].长春:吉林大学,2016
[27]赵春萍.姜黄素对大肠杆菌攻毒仔猪生长性能及肠粘膜屏障功能的影响[D].海南:海南大学,2015
[28]陈娜娜,马莲香,侯川川,何俊娜,郭阳,刘兵,余东游.蛋氨酸锌对蛋鸡生产性能、肠道形态、组织学结构及盲肠微生物菌群的影响[J].中国畜牧杂志,2017,53(9):102-108
[29]黄春兰,曾悦.杯状细胞及肠道黏液屏障的功能研究[J].国际消化病杂志,2017,37(6):357-360
[30] Takizawa Y,Kishimoto H,Kitazato T,Tomita M,Hayashi M.Changes in protein and m RNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion[J].International Journal of Pharmaceutics,2012,426(1):82-89
[2]陈会丛,翟建英,张广平,朱晓光,杨海润,张思玉,蒋威,李晋生.葡萄籽提取物原花青素一般药理学研究[J].中国中医药信息杂志,2014,21(3):36-39
[3] Koga T, Moro K, Nakamori K, Yamakoshi J, Hosoyama H,Kataoka S,Ariga T. Increase of antioxidative potential of rat plasma by oral administration of proanthocyanidin-rich extract from grape seeds[J]. Journal of Agricultural and Food Chemistry,1999,47(5):1892-1897
[4]凌智群.莲房原花青素及其生物、药理活性研究[D].武汉:华中农业大学,2001
[5] Ou K,Gu L. Absorption and metabolism of proanthocyanidins[J].Journal of Functional Foods,2014,7(1):43-53
[6] Deitch E A. Bacterial translocation or lymphatic drainage of toxic products from the gut:Whatis important in human beings[J].Surgery,2012,131(3):241-244
[7]王楠楠,白英龙.与肥胖相关的慢性炎症机制研究进展[J].中国全科医学,2017,20(12):1527-1530
[8] Kamel C,Mohamed M,Takwa B,Khawla J,Veronique E M M,Ferid L,Ezzedine A. Safety evaluation,anti-oxidative and antiinflammatory effects of subchronically dietary supplemented high dosing grape seed powder(GSP)to healthy rat[J]. Biomedicine&Pharmacotherapy,2018,107:534-546
[9]温馨,王兴亚,孙崇德,李鲜.黄酮类化合物调节肥胖相关炎症的研究进展[J].中草药,2017,48(14):2972-2978
[10]宣琪,张育先,刘殿刚,崔叶青.原花青素对高脂饮食诱导大鼠脂肪肝的防治研究[J].中华实用诊断与治疗杂志,2016,30(9):848-850
[11]刘桂.玉米肽结合有氧运动对高脂饮食诱导的肥胖大鼠体脂、血脂及脂肪分解关键酶ATGL、LPL的影响[D].上海:上海体育学院,2014
[12] Travis S,MenziesⅠ. Intestinal permeability:Functional assessment and significance[J]. Clinical Science,1992,82(5):471-488
[13]乔小东,武华.门静脉高压大鼠肠黏膜通透性改变时D-乳酸和内毒素的表达及复合膳食纤维的保护作用[J].中国医药导报,2014,11(9):26-28,32
[14]于博昊,杜一平,谢小波,栾绍嵘.离子色谱法检测幼兔尿样中的乳果糖与甘露醇[J].分析试验室,2010,29(3):119-121
[15]马维娟,许建明,胡静,刘晓昌,梅俏.肝硬化患者肠道粘膜通透性及其与Child-Pugh分级相关性的研究[J].中华疾病控制杂志,2015,19(4):411-414
[16]姜海龙,秦贵信,车东升,谷琳琳,张海全,赵金波.精氨酸对仔猪肠道机械屏障的影响及相关机理[J].吉林农业大学学报,2015,37(1):83-87
[17]李旋亮,李建涛,潘树德,吴长德.发酵饲料对断奶仔猪肠道肠黏膜形态的影响[J].饲料工业,2014,35(4):38-41
[18]张媛,杨晨玉,韩亚楠,陈耀星,王子旭,曹静,董玉兰. 5-羟色胺对断奶腹泻仔鼠小肠组织学结构的影响[J].中国农业大学学报,2017,22(2):80-86
[19]甘元涛,赵少勇.肠黏膜屏障损伤与紧密连接蛋白ZO-1的关系研究进展[J].现代医药卫生,2016,32(7):1027-1029
[20]邓宸玺,王自蕊,游金明,瞿明仁,叶亚玲,辛向荣,潘珂.丙氨酰-谷氨酰胺二肽对仔猪小肠上皮细胞间紧密连接蛋白occludin定位与表达的影响[J].动物营养学报,2014,26(3):694-700
[21]秘迎君.中国九省成人超重、肥胖的患病率及其与全死因死亡风险的关联研究[D].石家庄:河北医科大学,2017
[22]龚芳,艾宇航,黄绍华,贵春梅,陈超.连续性血液净化对重症胰腺炎患者炎症因子、内毒素及肠道黏膜屏障功能的影响[J].现代生物医学进展,2017,17(10):1849-1851,1895
[23]王晓枫.抑制肠源性内毒素改善大鼠极限肝切除术预后的三重作用及机制[D].重庆:中国人民解放军陆军军医大学,2013
[24]董莉君.高脂饮食对不同年龄小鼠脂肪组织炎症的影响[D].新乡:新乡医学院,2016
[25] Berg R D. Bacterial translocation from the gastrointestinal tract[J].Trends in Microbiology,1995,3(4):149-154
[26]刘芳.从炎症角度解析中度高脂饮食诱导肥胖对内分泌代谢及骨骼肌功能的影响[D].长春:吉林大学,2016
[27]赵春萍.姜黄素对大肠杆菌攻毒仔猪生长性能及肠粘膜屏障功能的影响[D].海南:海南大学,2015
[28]陈娜娜,马莲香,侯川川,何俊娜,郭阳,刘兵,余东游.蛋氨酸锌对蛋鸡生产性能、肠道形态、组织学结构及盲肠微生物菌群的影响[J].中国畜牧杂志,2017,53(9):102-108
[29]黄春兰,曾悦.杯状细胞及肠道黏液屏障的功能研究[J].国际消化病杂志,2017,37(6):357-360
[30] Takizawa Y,Kishimoto H,Kitazato T,Tomita M,Hayashi M.Changes in protein and m RNA expression levels of claudin family after mucosal lesion by intestinal ischemia/reperfusion[J].International Journal of Pharmaceutics,2012,426(1):82-89