摘要
本研究研制了一款白藜芦醇-葡萄多酚复方美白口服液,评价其抗氧化活性和美白作用,并对机理进行初步探讨。抗氧化活性实验结果表明,美白口服液具有较强的抗氧化活性,不仅能还原铁离子,还对DPPH+、ABTS+、过氧化自由基均有显著的清除作用。对DPPH+、ABTS+的IC50分别为10.32±1.20μg/m L、87.06±9.52μg/m L;对过氧自由基的清除、吸收能力分别为1649.00±2.78μmol equiv. vit. C/g、4251.30±566.71μmol of trolox equivalents (TE)/g。以人肝癌细胞Hep G2为细胞模型,美白口服液的胞内抗氧化活性可达5570.10±312.09μmol of quercetin equivalents (QE)/100 g。选择与人体黑色素细胞生理上较为接近的小鼠黑色素瘤B16细胞为研究模型,进行体外美白作用评价。结果表明,在一定添加量下,美白液能抑制B16细胞增殖、胞酪氨酸酶活性和蛋白表达量,从而减少黑色素的生成。这款复方美白口服液具有良好的抗氧化性与美白作用,将在功能食品、保健品行业有较好的应用前景。
This study developed a resveratrol-grape polyphenol composite whitening oral solution, evaluated its antioxidant activity and whitening effect, and discussed preliminarily the underlying mechanism. The results of the antioxidant activity experiments showed that the whitening oral solution exhibited strong antioxidant activity: not only reduced iron ions, but also exerted significant scavenging effects on the DPPH+ and ABTS+ free radicals and peroxides. The IC50 values for DPPH+ and ABTS+ radical scavenging activities were 10.32±1.20 μg/m L and 87.06±9.52 μg/m L, respectively. The peroxyl radical scavenging and absorption capacities were 1649.00±2.78 μmol Vit. C equivalents/g and 4251.30±566.71 μmol Trolox equivalents(TE)/g, respectively. In human hepatoma cell line Hep G2 model, the intracellular antioxidant activity of the whitening oral solution reached 5570.10±312.09 μmol quercetin equivalents(QE)/100 g. The mouse melanoma B16 cells, which are physiologically relevant to the human melanocytes, were selected as the research models to evaluate in vitro the whitening effect. The results showed that at a certain dose, the whitening oral solution could inhibit the proliferation of B16 cells, cellular tyrosinase activity and protein expression of B16 cells, thereby reducing the production of melanin. This composite whitening oral solution exhibited good antioxidant activity and whitening effect, thus, will have great prospects for the functional foods and healthcare products industry.
引文
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