略阳乌鸡内源性逆转录病毒鉴定及表达分析
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摘要
内源性禽白血病病毒等以多种逆转录病毒基因组稳定存在鸡染色体中,其致病性低或无致病性,但是内源性病毒的基因表达降低了鸡抵抗外源病毒感染的能力。本研究以略阳乌鸡为研究对象,利用多重PCR鉴定鸡基因组中禽内源性病毒EAV-HP和禽白血病病毒E亚群(ALV-E),分析ALV-E的env基因表达情况。结果表明,30只略阳乌鸡的基因组中均携带EAV-HP病毒序列,其中28只携带ALV-E序列。通过MEGA和NETWORK软件对ALV-E的gp85基因分析表明,略阳乌鸡与江苏绿壳蛋鸡聚在同一分支,两者同源性高达99.1%。此研究也为后续略阳乌鸡遗传背景分析和选育抗病毒感染优良品种提供基础数据。
Endogenous avian leukosis virus and others as retroviral genomes stablely present in chicken chromosomes,and their pathogenicity is low or no pathogenicity.However,endogenous viral gene expression reduces the ability of chicken to resist exogenous virus infection.This study took Lueyang black-bone chicken as a research object,EAV-HP and avian leukemia virus ALV-E of Lueyang black-bone chicken genome were identified by multiple PCR,and the expression of env gene in ALV-E was analyzed.The results demonstrated that EAV-HP virus sequences were found in genomes of 30 Lueyang black-bone chickens,and 28 of 30 chickens harbored ALV-E virus sequence.The results of gp85 sequences analysis via MEGA and NETWORK softwares illustrated that Lueyang black-bone chicken and Jiangsu green eggshell chicken were clustered in the same clade,with homology up to 99.1%.This study provided the basic data for the subsequent analysis of genetic background of Lueyang black-bone chicken and selection of good breeds with antiviral ability.
Endogenous avian leukosis virus and others as retroviral genomes stablely present in chicken chromosomes,and their pathogenicity is low or no pathogenicity.However,endogenous viral gene expression reduces the ability of chicken to resist exogenous virus infection.This study took Lueyang black-bone chicken as a research object,EAV-HP and avian leukemia virus ALV-E of Lueyang black-bone chicken genome were identified by multiple PCR,and the expression of env gene in ALV-E was analyzed.The results demonstrated that EAV-HP virus sequences were found in genomes of 30 Lueyang black-bone chickens,and 28 of 30 chickens harbored ALV-E virus sequence.The results of gp85 sequences analysis via MEGA and NETWORK softwares illustrated that Lueyang black-bone chicken and Jiangsu green eggshell chicken were clustered in the same clade,with homology up to 99.1%.This study provided the basic data for the subsequent analysis of genetic background of Lueyang black-bone chicken and selection of good breeds with antiviral ability.
引文
Adkins H.B.,Blacklow S.C.,and Young J.A.,2001,Two functionally distinct forms of a retroviral receptor explain the nonreciprocal receptor interference among subgroups B,D,and E avian leukosis viruses,Journal of Virology,75(8):3520-3526
Bacon L.D.,Fulton J.E.,and Kulkarni G.B.,2004,Methods for evaluating and developing commercial chicken strains free of endogenous subgroup E avian leukosis virus,33(2):233-243
Bacon L.D.,Hunt H.D.,and Cheng H.H.,2000,A review of the development of chicken lines to resolve genes determining resistance to diseases,Poultry Science,79(8):1082
Bates P.,Rong L.,Varmus H.E.,Young J.A.,and Crittenden L.B.,1998,Genetic mapping of the cloned subgroup a avian sarcoma and leukosis virus receptor gene to the TVA locus.,Journal of Virology,72(3):2505
Cui Z.Z.,2016,Multiplicity of subgroups and viral quasispecies of avian leucosis viruses and its evolution under different selective pressures,Shengming Kexue(Chinese Bulletin of Life Sciences),28(3):283-294(崔治中,2016,禽白血病病毒的亚群和准种多样性及其在不同选择压作用下的演变,生命科学,28(3):283-294)
Cui Z.,Du Y.,Zhang Z.,and Silva R.F.,2003,Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains,Avian Dis.,47(4):1321-1330
Jiang Y.P.,Wang Y.,Yu L.L.,Cai L.M.,Wang Z.Z.,Xu Q.Q.,Wang G.H.,and Cheng Z.Q.,2012,Clinical cases analysis of avian Leukosis subgroup J induced erythroblastosis in chicken,Xumu Shouyi Xuebao(Acta Veterinaria et Zootechnica Sinica),43(8):1317-1323(姜艳萍,王玥,于琳琳,蔡黎明,王真真,徐晴晴,王桂花,成子强,2012,ALV-J诱发鸡成红细胞白血病的临床病例分析,畜牧兽医学报,43(8):1317-1323)
Sacco M.A.,Flannery D.M.J.,Howes K.,and Venugopal K.,2000,Avian endogenous retrovirus EAVHP shares regions of identity with avian Leukosis virus subgroup J and the avian retrotransposon ART-CH,Journal of Virology,74(3):1296-1306
Melanie A.,and Sacco K.H.L.P.,2004,Assessing the roles of endogenous retrovirus EAV-HP in avian Leukosis virus subgroup J emergence and tolerance,Journal of Virology,78(19):10525-10535
Sacco M.A.,and Nair V.K.,2014,Prototype endogenous avian retroviruses of the genus Gallus,Journal of General Virology,95(9):2060-2070
Sacco M.A.,and Venugopal K.,2001,Segregation of EAV-HPancient endogenous retroviruses within the chicken population,Journal of Virology,75(23):11935-11938
Saif Y.,Barnes H.J.,Glisson J.R.,Fadly A.M.,Mcdougald L.R.,Swayne D.E.,Saif Y.,Barnes H.J.,Glisson J.R.,and Fadly A.M.,2003,Diseases of poultry,Australian Veterinary Journal,47(5):123-127
Sun S.,and Cui Z.,2007,Epidemiological and pathological studies of subgroup J avian leukosis virus infections in Chinese local“yellow”chickens,Avian Pathology,36(3):221
Tan L.Q.,Feng M.,Hao J.Y.,Qin J.R.,Liao M.,and Cao W.S.,2015,Isolation and genetic identification of endogenous avian leukosis virus strain from Albumen of partridge breeder Chicken,Zhongguo Shouyi Zazhi(Chinese Journal of Veterinary Medicine),51(3):3-5(谭利强,冯敏,郝建勇,秦建如,廖明,曹伟胜,2015,麻黄肉种鸡蛋清中内源性禽白血病病毒的分离与遗传鉴定,中国兽医杂志,51(3):3-5)
Wang Z.,Qu L.,Yao J.,Yang X.,Li G.,Zhang Y.,Li J.,Wang X.,Bai J.,and Xu G.,2013,An EAV-HP insertion in 5'flanking region of SLCO1B3 causes blue eggshell in the chicken,PLo S Genet.,9(1):e1003183
Wang Z.P.,Chen X.,Jiang Z.Q.,Liang X.W.,Li N.,and Liu F.Z.,2014,Analysis of molecular mechanisms underlying blue egg,rose-comb and dermal hyperpigmentation phenotypes in Lueyang blackbone chicken,Yichuan Yuzhong(Genetics and Breeding),50(15):6-9(王哲鹏,陈晓,蒋志强,梁肖伟,李娜,刘福柱,2014,略阳乌鸡绿壳蛋、玫瑰冠、乌皮性状遗传基础分析,遗传育种,50(15):6-9)
Yin Q.,Sun S.H.,Chai J.Q.,Guo H.J.,and Cui Z.Z.,2012,Studies on histopathology and ultrastructure of subgroup J-avian leukosis of“Bairi chicken”,Zhongguo Shouyi Xuebao(Chin.J.Vet.Sci.),32(2):290-295(尹青,孙淑红,柴家前,郭慧君,崔治中,2012,百日鸡J-亚群禽白血病组织病理学与超微病理学研究,中国兽医学报,32(2):290-295)
Bacon L.D.,Fulton J.E.,and Kulkarni G.B.,2004,Methods for evaluating and developing commercial chicken strains free of endogenous subgroup E avian leukosis virus,33(2):233-243
Bacon L.D.,Hunt H.D.,and Cheng H.H.,2000,A review of the development of chicken lines to resolve genes determining resistance to diseases,Poultry Science,79(8):1082
Bates P.,Rong L.,Varmus H.E.,Young J.A.,and Crittenden L.B.,1998,Genetic mapping of the cloned subgroup a avian sarcoma and leukosis virus receptor gene to the TVA locus.,Journal of Virology,72(3):2505
Cui Z.Z.,2016,Multiplicity of subgroups and viral quasispecies of avian leucosis viruses and its evolution under different selective pressures,Shengming Kexue(Chinese Bulletin of Life Sciences),28(3):283-294(崔治中,2016,禽白血病病毒的亚群和准种多样性及其在不同选择压作用下的演变,生命科学,28(3):283-294)
Cui Z.,Du Y.,Zhang Z.,and Silva R.F.,2003,Comparison of Chinese field strains of avian leukosis subgroup J viruses with prototype strain HPRS-103 and United States strains,Avian Dis.,47(4):1321-1330
Jiang Y.P.,Wang Y.,Yu L.L.,Cai L.M.,Wang Z.Z.,Xu Q.Q.,Wang G.H.,and Cheng Z.Q.,2012,Clinical cases analysis of avian Leukosis subgroup J induced erythroblastosis in chicken,Xumu Shouyi Xuebao(Acta Veterinaria et Zootechnica Sinica),43(8):1317-1323(姜艳萍,王玥,于琳琳,蔡黎明,王真真,徐晴晴,王桂花,成子强,2012,ALV-J诱发鸡成红细胞白血病的临床病例分析,畜牧兽医学报,43(8):1317-1323)
Sacco M.A.,Flannery D.M.J.,Howes K.,and Venugopal K.,2000,Avian endogenous retrovirus EAVHP shares regions of identity with avian Leukosis virus subgroup J and the avian retrotransposon ART-CH,Journal of Virology,74(3):1296-1306
Melanie A.,and Sacco K.H.L.P.,2004,Assessing the roles of endogenous retrovirus EAV-HP in avian Leukosis virus subgroup J emergence and tolerance,Journal of Virology,78(19):10525-10535
Sacco M.A.,and Nair V.K.,2014,Prototype endogenous avian retroviruses of the genus Gallus,Journal of General Virology,95(9):2060-2070
Sacco M.A.,and Venugopal K.,2001,Segregation of EAV-HPancient endogenous retroviruses within the chicken population,Journal of Virology,75(23):11935-11938
Saif Y.,Barnes H.J.,Glisson J.R.,Fadly A.M.,Mcdougald L.R.,Swayne D.E.,Saif Y.,Barnes H.J.,Glisson J.R.,and Fadly A.M.,2003,Diseases of poultry,Australian Veterinary Journal,47(5):123-127
Sun S.,and Cui Z.,2007,Epidemiological and pathological studies of subgroup J avian leukosis virus infections in Chinese local“yellow”chickens,Avian Pathology,36(3):221
Tan L.Q.,Feng M.,Hao J.Y.,Qin J.R.,Liao M.,and Cao W.S.,2015,Isolation and genetic identification of endogenous avian leukosis virus strain from Albumen of partridge breeder Chicken,Zhongguo Shouyi Zazhi(Chinese Journal of Veterinary Medicine),51(3):3-5(谭利强,冯敏,郝建勇,秦建如,廖明,曹伟胜,2015,麻黄肉种鸡蛋清中内源性禽白血病病毒的分离与遗传鉴定,中国兽医杂志,51(3):3-5)
Wang Z.,Qu L.,Yao J.,Yang X.,Li G.,Zhang Y.,Li J.,Wang X.,Bai J.,and Xu G.,2013,An EAV-HP insertion in 5'flanking region of SLCO1B3 causes blue eggshell in the chicken,PLo S Genet.,9(1):e1003183
Wang Z.P.,Chen X.,Jiang Z.Q.,Liang X.W.,Li N.,and Liu F.Z.,2014,Analysis of molecular mechanisms underlying blue egg,rose-comb and dermal hyperpigmentation phenotypes in Lueyang blackbone chicken,Yichuan Yuzhong(Genetics and Breeding),50(15):6-9(王哲鹏,陈晓,蒋志强,梁肖伟,李娜,刘福柱,2014,略阳乌鸡绿壳蛋、玫瑰冠、乌皮性状遗传基础分析,遗传育种,50(15):6-9)
Yin Q.,Sun S.H.,Chai J.Q.,Guo H.J.,and Cui Z.Z.,2012,Studies on histopathology and ultrastructure of subgroup J-avian leukosis of“Bairi chicken”,Zhongguo Shouyi Xuebao(Chin.J.Vet.Sci.),32(2):290-295(尹青,孙淑红,柴家前,郭慧君,崔治中,2012,百日鸡J-亚群禽白血病组织病理学与超微病理学研究,中国兽医学报,32(2):290-295)