杂交兰无菌播种及根状茎培养技术研究
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摘要
以杂交兰品种台北小姐与小叶虎头兰杂交果荚、台北小姐自交果荚作为材料,比较了不同活性炭浓度对其种子萌发的影响,以及二者种子萌发形成的根状茎在同一种培养基中的增殖与分化情况。结果表明:添加活性炭1.0g·L~(-1)的培养基较适宜台北小姐自交组合种子无菌萌发,而台北小姐与小叶虎头兰杂交组合种子无菌萌发较适宜的培养基则为不添加活性炭;在1/2MS+6-BA 0.5mg·L~(-1)+IBA 0.1mg·L~(-1)+活性炭1.0g·L~(-1)培养基中,台北小姐自交组合根状茎的增殖效果较好,而台北小姐与小叶虎头兰杂交组合根状茎分化较好。
By taking the hybrid fruit pod of Taibeixiaojie and Cymbidium hookerianum a kind of cymbidiumhybrid,and the self-pollinated fruit pod of Taipeixiaojie as the materials,the effects of different concentrations of activated carbon on the seed germination were compared.And the proliferation and differentiation of rhizomes formed by their seed germination in the same culture medium were also compared.The results showed that the culture medium with 1.0 g·L~(-1) activated carbon was suitable for the sterile germination of the seeds of self-pollinated combination of Taibeixiaojie,while the culture medium with no activated carbon was suitable for the sterile germination of the seeds of hybrid combination of Taibeixiaojie and Cymbidium hookerianum.Moreover,the culture medium with 1/2 MS+6-BA 0.5 mg·L~(-1)+IBA 0.1 mg·L~(-1)+no activater carbon 1.0 g·L~(-1) had better effect on the rhizome proliferation of self-pollinated combination of Taibeixiaojie,while it had better effect on the rhizome differentiation of hybrid combination of Taibeixiaojie and Cymbidium hookerianum.
By taking the hybrid fruit pod of Taibeixiaojie and Cymbidium hookerianum a kind of cymbidiumhybrid,and the self-pollinated fruit pod of Taipeixiaojie as the materials,the effects of different concentrations of activated carbon on the seed germination were compared.And the proliferation and differentiation of rhizomes formed by their seed germination in the same culture medium were also compared.The results showed that the culture medium with 1.0 g·L~(-1) activated carbon was suitable for the sterile germination of the seeds of self-pollinated combination of Taibeixiaojie,while the culture medium with no activated carbon was suitable for the sterile germination of the seeds of hybrid combination of Taibeixiaojie and Cymbidium hookerianum.Moreover,the culture medium with 1/2 MS+6-BA 0.5 mg·L~(-1)+IBA 0.1 mg·L~(-1)+no activater carbon 1.0 g·L~(-1) had better effect on the rhizome proliferation of self-pollinated combination of Taibeixiaojie,while it had better effect on the rhizome differentiation of hybrid combination of Taibeixiaojie and Cymbidium hookerianum.
引文
[1]胡蕾,申婷.杂交兰的组织培养与快繁技术[J].浙江农业科学,2017,58(2):259-260.
[2]蓝炎阳,钟淮钦,陈南川,等.大花蕙兰与墨兰种间杂交种子无菌播种繁殖技术研究[J].中国农学通报,2017,33(2):61-66.
[3]陈瑶瑶,张燕,张琛,等.杂交兰‘韩国桃花’×蕙兰种间杂交种子无菌萌发特征研究[J].园艺学报,2009,36(3):441-446.
[4]张先云,袁秀云,马杰.杂交兰的组织培养与快速繁殖技术研究[J].河南科学,2019,27(4):419-421.
[5]左利娟,李志强,郑志勇,等.杂交兰根状茎的增殖与分化成苗技术[J].江苏农业科学,2015,43(6):54-56.
[6]曾碧玉,常强,许传俊.杂交兰品种‘韩国小姐’与墨兰品种‘企剑黑墨’杂交坐果及无菌播种研究[J].热带作物学报,2015,36(3):510-515.
[7]郑君爽,宁惠娟,吕慧,等.国兰与大花蕙兰杂交育种及无菌播种研究进展[J].中国农学通报,2011,27(4):81-84.
[2]蓝炎阳,钟淮钦,陈南川,等.大花蕙兰与墨兰种间杂交种子无菌播种繁殖技术研究[J].中国农学通报,2017,33(2):61-66.
[3]陈瑶瑶,张燕,张琛,等.杂交兰‘韩国桃花’×蕙兰种间杂交种子无菌萌发特征研究[J].园艺学报,2009,36(3):441-446.
[4]张先云,袁秀云,马杰.杂交兰的组织培养与快速繁殖技术研究[J].河南科学,2019,27(4):419-421.
[5]左利娟,李志强,郑志勇,等.杂交兰根状茎的增殖与分化成苗技术[J].江苏农业科学,2015,43(6):54-56.
[6]曾碧玉,常强,许传俊.杂交兰品种‘韩国小姐’与墨兰品种‘企剑黑墨’杂交坐果及无菌播种研究[J].热带作物学报,2015,36(3):510-515.
[7]郑君爽,宁惠娟,吕慧,等.国兰与大花蕙兰杂交育种及无菌播种研究进展[J].中国农学通报,2011,27(4):81-84.