水杨酸缓解白苦瓜幼苗铝毒害的生理机制研究
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摘要
为了探明外源水杨酸对白苦瓜幼苗铝害胁迫缓解的生理机制,以400 mg/L的AlCl_3胁迫为对照,设置了3个处理(T_1:400 mg/L AlCl_3+30μmol/L SA;T_2:400 mg/L AlCl_3+60μmol/L SA;T_3:400 mg/L AlCl_3+90μmol/L SA),研究了不同浓度的水杨酸处理对铝胁迫下白苦瓜幼苗生理特性和生长状况的影响。结果表明:60μmol/L SA对白苦瓜幼苗铝害胁迫的缓解效果更为明显。在该浓度处理下,铝胁迫下白苦瓜幼苗的抗氧化酶活性和根系活力均显著升高,MDA含量显著下降,白苦瓜幼苗的生物量也显著高于对照。外施适宜浓度的SA后,铝胁迫下白苦瓜幼苗的根系受到了一定的保护,膜脂过氧化作用受到抑制,光合膜的损害有所缓解,从而促进了铝胁迫下白苦瓜幼苗无机物的吸收和有机物的合成。
In order to ascertain the physiological mechanism of mitigation of exogenous salicylic acid( SA) on aluminum toxicity stress to white balsam pear seedlings,taking 400 mg/L AlCl_3 stress as the control,setting up the following 3 treatments: T_1,400 mg/L AlCl_3+ 30 μmol/L SA; T_2,400 mg/L AlCl_3+ 60 μmol/L SA; T_3,400 mg/L AlCl_3+ 90 μmol/L SA,the author studied the effects of different concentrations of SA on the physiological characteristics and growth status of white balsam pear seedlings under aluminum stress. The results showed that the mitigation effect of 60 μmol/L SA on the aluminum stress to white balsam pear seedlings was more obvious. Under the treatment with 60 μmol/L SA,both the activities of antioxidant enzymes and the root activity of white balsam pear seedlings under aluminum stress increased significantly,while the MDA content decreased significantly,and the biomass of seedlings was also significantly higher than that of the control. After applying suitable concentration of SA,the roots of white balsam pear seedlings under aluminum stress were protected,the membrane lipid peroxidation was inhibited,and the damage to photosynthetic membrane was also remitted to some extent,which promoted the absorption of inorganic substances and the synthesis of organic substances in the seedlings of white balsam pear under aluminum stress.
In order to ascertain the physiological mechanism of mitigation of exogenous salicylic acid( SA) on aluminum toxicity stress to white balsam pear seedlings,taking 400 mg/L AlCl_3 stress as the control,setting up the following 3 treatments: T_1,400 mg/L AlCl_3+ 30 μmol/L SA; T_2,400 mg/L AlCl_3+ 60 μmol/L SA; T_3,400 mg/L AlCl_3+ 90 μmol/L SA,the author studied the effects of different concentrations of SA on the physiological characteristics and growth status of white balsam pear seedlings under aluminum stress. The results showed that the mitigation effect of 60 μmol/L SA on the aluminum stress to white balsam pear seedlings was more obvious. Under the treatment with 60 μmol/L SA,both the activities of antioxidant enzymes and the root activity of white balsam pear seedlings under aluminum stress increased significantly,while the MDA content decreased significantly,and the biomass of seedlings was also significantly higher than that of the control. After applying suitable concentration of SA,the roots of white balsam pear seedlings under aluminum stress were protected,the membrane lipid peroxidation was inhibited,and the damage to photosynthetic membrane was also remitted to some extent,which promoted the absorption of inorganic substances and the synthesis of organic substances in the seedlings of white balsam pear under aluminum stress.
引文
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[12]张永福,任禛,陈泽斌,等.水杨酸对缓解葡萄苗铝毒害的生理机制[J].华北农学报,2015,30(1):182-187.
[13]王玉宁.苦瓜中的白雪公主——白苦瓜[J].烹调知识,2017(8):19.
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[16]朱红芳,高倩倩,李晓锋,等.根肿病胁迫下外源SA对不结球白菜抗性诱导和生理生化的影响[J].西北植物学报,2017,37(2):297-306.
[17]陈文笔,张琳,徐继林,等.外源水杨酸提高微拟球藻低温抗逆性的应用[J].农业生物技术学报,2017,25(2):240-249.
[18]Blancaflor,E B,Jones,D L,Gilroy S.Alterations in the cytoskeleton accompany aluminum-induced growth inhibition and morphological changes in primary roots of maize[J].Plant Physiol,1998,118(1):159-72.
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[20]张蕊,吕俊,米青山,等.低温下外源水杨酸对水稻幼苗抗氧化酶系的影响[J].西南农业大学学报:自然科学版,2006(1):29-32,36.
[21]郝敬虹,易旸,尚庆茂,等.干旱胁迫下外源水杨酸对黄瓜幼苗膜脂过氧化和光合特性的影响[J].应用生态学报,2012,23(3):717-723.
[22]崔秀妹.水分胁迫下水杨酸对扁蓿豆生长、光合生理及饲草产量和品质的影响[D].南京:南京农业大学,2012.
[23]李明.外源水杨酸对花椒光合作用及抗旱能力的影响[D].兰州:甘肃农业大学,2010.
[2]王宝增,张一名,张江丽,等.水杨酸对盐胁迫下沙打旺幼苗生长的影响[J].草业学报,2016,25(8):74-80.
[3]吴超,郭方其,陈世平,等.外源水杨酸(SA)对食用百合长期低温储藏过程中生理代谢影响[J].分子植物育种,2016,14(9):2495-2501.
[4]Khan W,Prithiviraj B,Smith D L.Photosynthetic responses of corn and soybean to foliar application of salicylates[J].Journal of Plant Physiology,2003,160(5):485-492.
[5]Rivassan Vicente M,Plasencia J.Salicylic acid beyond defence:its role in plant growth and development[J].Journal of Experimental Botany,2011,62(10):3321-3338.
[6]罗承德,张健,刘继龙.四川盆周山地杉木人工林衰退与铝毒害阈值的探讨[J].林业科学,2000,36(1):9-14.
[7]闫世才,毛学文,杨勇理.铝对豌豆生长的影响[J].生态学杂志,2003,22(2):80-81.
[8]Rout G R,Samantaray S,Das P.Aluminium toxicity in plants:a review[J].Agronomie,2001,21(1):3-21.
[9]赵会娥,贺立源,章爱群,等.铝胁迫对植物光合作用的影响及其机理的研究进展[J].华中农业大学学报,2008,27(1):155-160.
[10]许中坚,徐冬梅,刘广深,等.红壤中铝、锰和铁在酸雨作用下的释放特征[J].水土保持学报,2004,18(3):20-23,27.
[11]曹林,吴玉环,章艺,等.外源水杨酸对铝胁迫下菊芋光合特性及耐铝性的影响[J].水土保持学报,2015,29(4):260-266.
[12]张永福,任禛,陈泽斌,等.水杨酸对缓解葡萄苗铝毒害的生理机制[J].华北农学报,2015,30(1):182-187.
[13]王玉宁.苦瓜中的白雪公主——白苦瓜[J].烹调知识,2017(8):19.
[14]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000.
[15]郝再彬,苍晶,徐仲.植物生理实验[M].哈尔滨:哈尔滨工业大学出版社,2004.
[16]朱红芳,高倩倩,李晓锋,等.根肿病胁迫下外源SA对不结球白菜抗性诱导和生理生化的影响[J].西北植物学报,2017,37(2):297-306.
[17]陈文笔,张琳,徐继林,等.外源水杨酸提高微拟球藻低温抗逆性的应用[J].农业生物技术学报,2017,25(2):240-249.
[18]Blancaflor,E B,Jones,D L,Gilroy S.Alterations in the cytoskeleton accompany aluminum-induced growth inhibition and morphological changes in primary roots of maize[J].Plant Physiol,1998,118(1):159-72.
[19]张帆,罗承德,张健.植物铝胁迫发生机制及其内在缓解途径研究进展[J].四川环境,2005,24(3):64-69.
[20]张蕊,吕俊,米青山,等.低温下外源水杨酸对水稻幼苗抗氧化酶系的影响[J].西南农业大学学报:自然科学版,2006(1):29-32,36.
[21]郝敬虹,易旸,尚庆茂,等.干旱胁迫下外源水杨酸对黄瓜幼苗膜脂过氧化和光合特性的影响[J].应用生态学报,2012,23(3):717-723.
[22]崔秀妹.水分胁迫下水杨酸对扁蓿豆生长、光合生理及饲草产量和品质的影响[D].南京:南京农业大学,2012.
[23]李明.外源水杨酸对花椒光合作用及抗旱能力的影响[D].兰州:甘肃农业大学,2010.