外源水杨酸对喜树幼苗盐害胁迫缓解效应的生理机制初探
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摘要
为了明确外源水杨酸对喜树幼苗盐害胁迫缓解效应的生理机制,以喜树幼苗为试验材料,以0.5%NaCl胁迫为对照,设4个处理:T1(0.5%NaCl+60 mg/L SA喷叶),T2(0.5%NaCl+60 mg/L SA灌根),T3(0.5%NaCl+120 mg/L SA喷叶),T4(0.5%NaCl+120 mg/L SA灌根)。通过比较不同处理间喜树幼苗的超氧化物歧化酶活性、过氧化物酶活性、可溶性蛋白含量、丙二醛含量以及干重发现:60 mg/L的水杨酸处理对喜树幼苗NaCl胁迫的缓解作用效果最佳,且喷叶处理的效果优于灌根处理。60 mg/L水杨酸喷叶处理盐胁迫下喜树幼苗叶片中的保护酶活性和渗透调节物质含量均显著增加,叶片中的丙二醛含量显著下降,喜树幼苗的干重则显著高于对照。表明外施适宜浓度的水杨酸可增强盐胁迫下喜树幼苗的抗氧化物酶活性并增加渗透调节物质含量,进而保护了植物光合膜的完整性,提高了细胞的保水能力,并最终促进了盐胁迫下喜树幼苗的生长。通过研究水杨酸缓解喜树幼苗盐害的生理机制,为喜树的抗盐栽培提供了一些理论依据和参考。
To determine the physiological mechanism of mitigative effects of salicylic acid on salt-stressed Camptotheca acuminata seedlings, under the control of 0.5% NaCl stress, 4 treatments were set up: T1(0.5% NaCl+60 mg/L SA foliar spray), T2(0.5% NaCl+60 mg/L SA root irrigation), T3(0.5% NaCl+120 mg/L SA foliar spray) and T4(0.5% NaCl+120 mg/L SA root irrigation). Through the comparison of Camptotheca acuminata seedlings′ antioxi-dant enzyme activity, soluble protein content, MDA content as well as dry weight, we found that the mitigative ef-fects of 60 mg/L SA was best, and the result of foliar spraying was better than that of root irrigating. Under the treat-ment of 60 mg/L SA foliar spraying, activity of protective enzymes in leaves of Camptotheca acuminata seedlingsand content of osmotic adjustment substances increased significantly, MDA content decreased significantly, the dryweights of seedlings were higher than those of control significantly. This showed that application of salicylic acid atsuitable concentration could enhance antioxidant enzyme activity, increase the content of osmotic adjustment sub-stances, thus protecting the integrity of the photosynthetic membrane, increasing cell water retention capacity, andultimately promoting the growth of seedlings under salt stress. By studying the physiological mechanism of mitiga-tive effects of salicylic acid on salt-stressed Camptotheca acuminata seedlings, theoretical basis was provided forsalt tolerance cultivation of Camptotheca acuminata.
To determine the physiological mechanism of mitigative effects of salicylic acid on salt-stressed Camptotheca acuminata seedlings, under the control of 0.5% NaCl stress, 4 treatments were set up: T1(0.5% NaCl+60 mg/L SA foliar spray), T2(0.5% NaCl+60 mg/L SA root irrigation), T3(0.5% NaCl+120 mg/L SA foliar spray) and T4(0.5% NaCl+120 mg/L SA root irrigation). Through the comparison of Camptotheca acuminata seedlings′ antioxi-dant enzyme activity, soluble protein content, MDA content as well as dry weight, we found that the mitigative ef-fects of 60 mg/L SA was best, and the result of foliar spraying was better than that of root irrigating. Under the treat-ment of 60 mg/L SA foliar spraying, activity of protective enzymes in leaves of Camptotheca acuminata seedlingsand content of osmotic adjustment substances increased significantly, MDA content decreased significantly, the dryweights of seedlings were higher than those of control significantly. This showed that application of salicylic acid atsuitable concentration could enhance antioxidant enzyme activity, increase the content of osmotic adjustment sub-stances, thus protecting the integrity of the photosynthetic membrane, increasing cell water retention capacity, andultimately promoting the growth of seedlings under salt stress. By studying the physiological mechanism of mitiga-tive effects of salicylic acid on salt-stressed Camptotheca acuminata seedlings, theoretical basis was provided forsalt tolerance cultivation of Camptotheca acuminata.
引文
[1]王佳丽,黄贤金,钟太洋,等.盐碱地可持续利用研究综述[J].地理学报,2011,66(5):673-684.
[2]Gao F,Huang Q,Sun X Y,et al.Study on dynamic changes of the soil salinization in the upper stream of the Tarim river based on RS and GIS[J].Procedia Environmental Sciences,2011,11(12):1135-1141.
[3]周丹丹,刘德玺,李存华,等.盐胁迫对朴树和速生白榆幼苗光合特性及叶绿素荧光参数的影响[J].西北植物学报,2016,36(5):1004-1011.
[4]王玲丽,周晓君,刘文哲.增强UV-B辐射对喜树生理指标及喜树碱含量的影响(英文)[J].西北植物学报,2016,36(5):979-986.
[5]康菊清,张建强,李智军,等.陕西省国家重点保护植物—分布新记录种—喜树(Camptotheca acuminata)[J].陕西师范大学学报(自然科学版),2017,45(2):68-70.
[6]郝峰鸽,周俊国,周秀梅.Na Cl胁迫对喜树幼苗生长和叶片生理特性的影响[J].东北林业大学学报,2010,38(1):18-19.
[7]Hayat Q,Hayat S,Irfan M,et al.Effect of exogenous salicylic acid under changing environment:a review[J].Environmental&Experimental Botany,2010,68(1):14-25.
[8]代红军,秦晨亮,丁玲.水杨酸对‘赤霞珠’葡萄总类黄酮、白藜芦醇含量及相关酶活性的影响[J].中国农业大学学报,2016,21(7):37-42.
[9]徐晓昀,郁继华,颉建明,等.水杨酸和油菜素内酯对低温胁迫下黄瓜幼苗光合作用的影响[J].应用生态学报,2016,27(9):3009-3015.
[10]Yuan S,Lin H H.Role of salicylic acid in plant abiotic stress[J].Zeitschrift Für Naturforschung C,2008,63(5-6):313-320.
[11]吴超,郭方其,陈世平,等.外源水杨酸(SA)对食用百合长期低温储藏过程中生理代谢影响[J].分子植物育种,2016,14(9):2495-2501.
[12]刘广明,李金彪,王秀萍,等.外源水杨酸对黑麦草幼苗盐胁迫的缓解效应研究[J].土壤学报,2016,53(4):995-1002.
[13]王宝增,张一名,张江丽,等.水杨酸对盐胁迫下沙打旺幼苗生长的影响[J].草业学报,2016,25(8):74-80.
[14]唐艳萍,文涛,孙歆,等.水杨酸对植物光合作用影响的研究进展[J].西北植物学报,2015,35(8):1701-1708.
[15]许力,李姣,宋文强,等.水杨酸通过提高抗氧化酶活性及基因表达缓解番茄低钾胁迫[J].华北农学报,2016,31(1):96-101.
[16]董春娟,曹宁,尚庆茂.外源水杨酸对低温胁迫下黄瓜幼苗根系脂肪酸不饱和度的影响[J].园艺学报,2017,44(7):1319-1326.
[17]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:167-169.
[18]中国科学院上海植物生理研究所.现代植物生理学实验指南[M].北京:科学出版社,1999:236-238.
[19]李天来,李淼,孙周平.钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J].应用生态学报,2009,20(3):586-590.
[20]蒋光华,母少东,李浩,等.不同植烟区土壤对中上部烟叶抗氧化系统及部分质量指标的影响[J].土壤,2014,46(6):1157-1163.
[21]杨颖丽,马海荣,李晶,等.茶多酚对盐胁迫下小麦幼苗根渗透调节作用的影响[J].西北师范大学学报(自然科学版),2017,53(5):84-90.
[22]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002,22(4):503-507.
[23]扈雪欢,宁欢欢,刘光照,等.外源SA对盐胁迫下颠茄生理生化、氮代谢及次生代谢的影响[J].草业学报,2017,26(11):147-156.
[24]张会灵,吴正景,郭大龙,等.IAA和SA对盐胁迫下萝卜幼苗生理特性的影响[J].北方园艺,2017(8):32-34.
[25]杨洪兵,李发良.盐胁迫下川荞3号和川荞4号生理特性的比较[J].吉林农业科学,2014,39(3):14-17.
[26]李才生,秦燕,宗盼.水杨酸对玉米幼苗的生长及细胞膜的影响[J].吉林农业科学,2010,35(2):5-8.
[2]Gao F,Huang Q,Sun X Y,et al.Study on dynamic changes of the soil salinization in the upper stream of the Tarim river based on RS and GIS[J].Procedia Environmental Sciences,2011,11(12):1135-1141.
[3]周丹丹,刘德玺,李存华,等.盐胁迫对朴树和速生白榆幼苗光合特性及叶绿素荧光参数的影响[J].西北植物学报,2016,36(5):1004-1011.
[4]王玲丽,周晓君,刘文哲.增强UV-B辐射对喜树生理指标及喜树碱含量的影响(英文)[J].西北植物学报,2016,36(5):979-986.
[5]康菊清,张建强,李智军,等.陕西省国家重点保护植物—分布新记录种—喜树(Camptotheca acuminata)[J].陕西师范大学学报(自然科学版),2017,45(2):68-70.
[6]郝峰鸽,周俊国,周秀梅.Na Cl胁迫对喜树幼苗生长和叶片生理特性的影响[J].东北林业大学学报,2010,38(1):18-19.
[7]Hayat Q,Hayat S,Irfan M,et al.Effect of exogenous salicylic acid under changing environment:a review[J].Environmental&Experimental Botany,2010,68(1):14-25.
[8]代红军,秦晨亮,丁玲.水杨酸对‘赤霞珠’葡萄总类黄酮、白藜芦醇含量及相关酶活性的影响[J].中国农业大学学报,2016,21(7):37-42.
[9]徐晓昀,郁继华,颉建明,等.水杨酸和油菜素内酯对低温胁迫下黄瓜幼苗光合作用的影响[J].应用生态学报,2016,27(9):3009-3015.
[10]Yuan S,Lin H H.Role of salicylic acid in plant abiotic stress[J].Zeitschrift Für Naturforschung C,2008,63(5-6):313-320.
[11]吴超,郭方其,陈世平,等.外源水杨酸(SA)对食用百合长期低温储藏过程中生理代谢影响[J].分子植物育种,2016,14(9):2495-2501.
[12]刘广明,李金彪,王秀萍,等.外源水杨酸对黑麦草幼苗盐胁迫的缓解效应研究[J].土壤学报,2016,53(4):995-1002.
[13]王宝增,张一名,张江丽,等.水杨酸对盐胁迫下沙打旺幼苗生长的影响[J].草业学报,2016,25(8):74-80.
[14]唐艳萍,文涛,孙歆,等.水杨酸对植物光合作用影响的研究进展[J].西北植物学报,2015,35(8):1701-1708.
[15]许力,李姣,宋文强,等.水杨酸通过提高抗氧化酶活性及基因表达缓解番茄低钾胁迫[J].华北农学报,2016,31(1):96-101.
[16]董春娟,曹宁,尚庆茂.外源水杨酸对低温胁迫下黄瓜幼苗根系脂肪酸不饱和度的影响[J].园艺学报,2017,44(7):1319-1326.
[17]李合生,孙群,赵世杰,等.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:167-169.
[18]中国科学院上海植物生理研究所.现代植物生理学实验指南[M].北京:科学出版社,1999:236-238.
[19]李天来,李淼,孙周平.钙和水杨酸对亚高温胁迫下番茄叶片保护酶活性的调控作用[J].应用生态学报,2009,20(3):586-590.
[20]蒋光华,母少东,李浩,等.不同植烟区土壤对中上部烟叶抗氧化系统及部分质量指标的影响[J].土壤,2014,46(6):1157-1163.
[21]杨颖丽,马海荣,李晶,等.茶多酚对盐胁迫下小麦幼苗根渗透调节作用的影响[J].西北师范大学学报(自然科学版),2017,53(5):84-90.
[22]李明,王根轩.干旱胁迫对甘草幼苗保护酶活性及脂质过氧化作用的影响[J].生态学报,2002,22(4):503-507.
[23]扈雪欢,宁欢欢,刘光照,等.外源SA对盐胁迫下颠茄生理生化、氮代谢及次生代谢的影响[J].草业学报,2017,26(11):147-156.
[24]张会灵,吴正景,郭大龙,等.IAA和SA对盐胁迫下萝卜幼苗生理特性的影响[J].北方园艺,2017(8):32-34.
[25]杨洪兵,李发良.盐胁迫下川荞3号和川荞4号生理特性的比较[J].吉林农业科学,2014,39(3):14-17.
[26]李才生,秦燕,宗盼.水杨酸对玉米幼苗的生长及细胞膜的影响[J].吉林农业科学,2010,35(2):5-8.