糖基化鱼蛋白对大鼠盲肠菌群及抗氧化能力的影响
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摘要
目的:探讨糖基化鱼蛋白对高脂饮食大鼠盲肠菌群和抗氧化能力的影响。方法:45%高脂饮食喂养SD大鼠4周建立高脂血症模型,然后分别给予经过改良的45%高脂饮食,即含20%鱼蛋白的鱼蛋白对照饮食(FP)、含6%糖基化鱼蛋白的饮食(L-GP)以及含12%糖基化鱼蛋白的饮食(H-GP)4周,检测血浆中转氨酶含量以及血浆和肝脏中的抗氧化能力,同时利用16s rRNA高通量测序检测对盲肠菌群的影响。结果:与FP组比较,L-GP组能降低血浆中谷丙转氨酶(ALT)和丙二醛(MDA)水平(P<0.05);L-GP和H-GP组能提高血浆和肝组织中超氧化物歧化酶(SOD)的活力及肝脏中还原性谷胱甘肽(GSH)水平(P<0.05);L-GP组增加Ruminococcaceae_UCG-014和Roseburia等的相对丰度、减少Desulfovibrio和Fusobacterium等潜在有害菌的相对丰度。结论:摄食低水平的糖基化鱼蛋白可以提高大鼠的抗氧化能力,并且对肠道健康具有一定潜在的益生作用。
Purpose: The effect of glycated fish protein on the composition of cecal microbiota and antioxidant ability in rats with high-fat diet was investigated. Methods: Sprague Dawley rats were fed with 45% high-fat for 4 weeks to be induced hyperlipidemia model. Then the rats were fed the modified45% high-fat diet with 20% fish protein(FP control diet), 6% glycated fish protein(GP)(L-GP diet) and12% GP(H-GP diet) for 4 weeks, respectively. The level of the plasma aminotransferase, plasma and liver tissue antioxidant capacity were determined. And gut microbiota in cecal contents were detected by 16 s High-throughput rRNA sequencing. Results: Compared with the FP group, L-GP group could better reduce the level of the plasma alanine transaminase(ALT) and malondialdehyde(MDA)(P<0.05).Simultaneously, the L-GP group and H-GP group could significantly increase the vitality of superoxide dismutase(SOD) in the plasma and liver tissue(P<0.05), and increase the level of glutathione(GSH) in the liver tissue(P<0.05). Furthermore, the L-GP group could increase the relative abundance of beneficial bacteria Ruminococcaceae_UCG-014 and Roseburia, and decrease harmful bacteria Desulfovibrio and Fusobacterium. Conclusion: The low-level-glycated fish protein can improve the antioxidant capacity, and has a more potential beneficial effect on gut health.
Purpose: The effect of glycated fish protein on the composition of cecal microbiota and antioxidant ability in rats with high-fat diet was investigated. Methods: Sprague Dawley rats were fed with 45% high-fat for 4 weeks to be induced hyperlipidemia model. Then the rats were fed the modified45% high-fat diet with 20% fish protein(FP control diet), 6% glycated fish protein(GP)(L-GP diet) and12% GP(H-GP diet) for 4 weeks, respectively. The level of the plasma aminotransferase, plasma and liver tissue antioxidant capacity were determined. And gut microbiota in cecal contents were detected by 16 s High-throughput rRNA sequencing. Results: Compared with the FP group, L-GP group could better reduce the level of the plasma alanine transaminase(ALT) and malondialdehyde(MDA)(P<0.05).Simultaneously, the L-GP group and H-GP group could significantly increase the vitality of superoxide dismutase(SOD) in the plasma and liver tissue(P<0.05), and increase the level of glutathione(GSH) in the liver tissue(P<0.05). Furthermore, the L-GP group could increase the relative abundance of beneficial bacteria Ruminococcaceae_UCG-014 and Roseburia, and decrease harmful bacteria Desulfovibrio and Fusobacterium. Conclusion: The low-level-glycated fish protein can improve the antioxidant capacity, and has a more potential beneficial effect on gut health.
引文
[1]方伟.高脂膳食和氧化蛋白对小鼠肠道菌群和氧化还原状态的影响[D].无锡:江南大学,2012.
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[16]Potrykus J,Mahaney B,White R L,et al.Proteomic investigation of glucose metabolism in the butyrateproducing gut anaerobe Fusobacterium varium[J].Proteomics,2007,11(7):1839-1853.
[17]Balamurugan R,Rajendiran E,George S,et al.Realtime polymerase chain reaction quantification of specific butyrate-producing bacteria,Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer[J].Journal of Gastroenterology&Hepatology,2010,23(8pt1):1298-1303.
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[20]Arora S,Kaur I P,Chopra K,et al.Efficiency of double layered microencapsulated probiotic to modulate proinflammatory molecular markers for the management of alcoholic liver disease[J].Mediators Inflamm,2014,(1):715130.
[2]Power K A,Lepp D,Zarepoor L,et al.Dietary flaxseed modulates the colonic microenvironment in healthy C57Bl/6 male mice which may alter susceptibility to gut-associated diseases[J].Journal of Nutritional Biochemistry,2016,28:61-69.
[3]Mu C,Yang Y,Luo Z,et al.The Colonic Microbiome and Epithelial Transcriptome Are Altered in Rats Fed a HighProtein Diet Compared with a Normal-Protein Diet[J].Journal of Nutrition,2016,146(3):474-483.
[4]杨宇鸿,董士远,靳卫亚,等.草鱼肌原纤维蛋白-葡萄糖糖基化产物的理化特性及乳化特性研究[J].食品工业科技,2018,39(18):77-82,142.
[5]Han K,Jin W,Mao Z,et al.Microbiome and butyrate production are altered in the gut of rats fed a glycated fish protein diet[J].Journal of Functional Foods,2018.
[6]Fan P,Liu P,Song P,et al.Moderate dietary protein restriction alters the composition of gut microbiota and improves ileal barrier function in adult pig model[J].Scientific Reports,2017,(7):43412.
[7]Delgado-Andrade C,de la Cueva S P,Peinado M J,et al.Modifications in bacterial groups and short chain fatty acid production in the gut of healthy adult rats after longterm consumption of dietary Maillard reaction products[J].Food Research International,2017,100:134-142.
[8]Qu W,Yuan X,Zhao J,et al.Dietary advanced glycation end products modify gut microbial composition and partially increase colon permeability in rats][J].Molecular Nutrition&Food Research,2017:1700118.
[9]Huiming H,Suhong C,Guiyuan L,et al.Effect of Shenshao Hypolipidemic Tablet on blood lipid levels and liver fuction of hyperlipidemia mice[J].Pharmacology&Clinics of Chinese Materia Medica,2011,27(2):113-114.
[10]孙晓泽,谭高峰,刘爱华.消渴方加减对气阴两虚夹瘀型糖尿病肾病的内皮损伤、氧化应激及生化指标的影响[J].中国实验方剂学杂志,2019,25(9):43-48.
[11]Louis P,Flint H J.Formation of propionate and butyrate by the human colonic microbiota[J].Environmental microbiology,2017,19(1):29-41.
[12]An C,Kuda T,Yazaki T,et al.Caecal fermentation,putrefaction and microbiotas in rats fed milk casein,soy protein or fish meal[J].Applied microbiology and biotechnology,2014,98(6):2779-2787.
[13]高文文,孟祥晨.人肠道产丁酸细菌及其所产丁酸的促健康作用[J/OL].食品科学:1-10.http://kns.cnki.net/kcms/detail/11.2206.TS.20181214.1415.100.html.
[14]Michiels C,Raes M,Toussaint O,et al.Importance of Se-glutathione peroxidase,catalase,and Cu/Zn-SOD for cell survival against oxidative stress[J].Free Radic Biol Med,1994,17(3):235-248.
[15]Cai W.High Levels of Dietary Advanced Glycation End Products Transform Low-Density Lipoprotein Into a Potent Redox-Sensitive Mitogen-Activated Protein Kinase Stimulant in Diabetic Patients[J].Circulation,2004,110(3):285-291.
[16]Potrykus J,Mahaney B,White R L,et al.Proteomic investigation of glucose metabolism in the butyrateproducing gut anaerobe Fusobacterium varium[J].Proteomics,2007,11(7):1839-1853.
[17]Balamurugan R,Rajendiran E,George S,et al.Realtime polymerase chain reaction quantification of specific butyrate-producing bacteria,Desulfovibrio and Enterococcus faecalis in the feces of patients with colorectal cancer[J].Journal of Gastroenterology&Hepatology,2010,23(8pt1):1298-1303.
[18]Kostic A D,Gevers D,Pedamallu C S,et al.Genomic analysis identifies association of Fusobacterium with colorectal carcinoma[J].Genome Research,2012,22(2):292-298.
[19]Jin W,Han K,Dong S,et al.Modifications in gut microbiota and fermentation metabolites in the hindgut of rats after the consumption of galactooligosaccharide glycated with a fish peptide[J].Food&Function,2018,(9).
[20]Arora S,Kaur I P,Chopra K,et al.Efficiency of double layered microencapsulated probiotic to modulate proinflammatory molecular markers for the management of alcoholic liver disease[J].Mediators Inflamm,2014,(1):715130.