人工老化对老芒麦种子生理生化特性及醇溶蛋白组成的影响
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摘要
以老芒麦(Elymus sibiricus)种子为材料,采用高温(45℃)、高湿(100%RH)的方法处理供试种子,测定电导率、丙二醛、可溶性糖、可溶性蛋白的含量,SOD和POD活性等相关生理生化指标,并采用酸性聚丙烯酰氨凝胶电泳(A-PAGE)技术对不同程度老化种子的醇溶蛋白组成进行分析,研究人工老化对老芒麦种子生理生化特性及醇溶蛋白组成的影响。结果表明:随着人工老化程度的加重,种子浸出液电导率和MDA含量都呈现逐渐增加的趋势;可溶性蛋白含量呈现先上升后下降的趋势,老化3 d后,可溶性蛋白含量急剧下降,老化7 d后,可溶性蛋白含量与对照相比下降了11.89 mg/g;老化处理7 d后,可溶性糖含量与对照相比增加,增幅为79.05%;随着老化程度的加剧,SOD、POD活性都逐渐下降,老化7 d后,SOD和POD活性依次下降44.49%、32.62%;SOD活性下降大于POD活性下降。种子在老化过程中醇溶蛋白发生了一系列的变化,醇溶蛋白图谱颜色在处理的第3 d开始变浅,且随着老化时间的延长,α,β和ω区醇溶蛋白出现丢失的现象。
Elymus sibiricus seeds treated with high temperature(45℃) and high humidity(100% RH) were used as materials.The changes of physiological and biochemical indexes(electrical conductivity,malondialdehyde content,soluble sugar content,soluble protein content,SOD activity and POD activity) were measured and the constitute of gliadin was analyzed by acid-soluble polyacrylamide gel electrophoresis(A-PAGE) technique.The results showed that the conductivity and MDA content of seed showed a gradual increase trend with the increase of artificial aging degree;The soluble protein content showed a trend of increasing first and then decreasing and decreased sharply after aging for 3 days.Compared with the control,the content of soluble protein decreased by 11.89 mg/g after aging for 7 days.Compared with the control,the content of soluble sugar increased by 79.05% after aging treatment for 7 days;The activity of SOD and POD decreased gradually with the increase of artificial aging degree.The activity of SOD and POD decreased by 44.49% and 32.62% after aging for 7 days,respectively.The degree of SOD activity decreased was greater than that of POD activity(44.49%>32.62%) after aging treatment for the same time.During the aging process,the constitute of gliadin was changed.The color of gliadin band lightened on the third day and some gliadin bands disappeared in α,β,ω region with the increase of artificial aging degree.
Elymus sibiricus seeds treated with high temperature(45℃) and high humidity(100% RH) were used as materials.The changes of physiological and biochemical indexes(electrical conductivity,malondialdehyde content,soluble sugar content,soluble protein content,SOD activity and POD activity) were measured and the constitute of gliadin was analyzed by acid-soluble polyacrylamide gel electrophoresis(A-PAGE) technique.The results showed that the conductivity and MDA content of seed showed a gradual increase trend with the increase of artificial aging degree;The soluble protein content showed a trend of increasing first and then decreasing and decreased sharply after aging for 3 days.Compared with the control,the content of soluble protein decreased by 11.89 mg/g after aging for 7 days.Compared with the control,the content of soluble sugar increased by 79.05% after aging treatment for 7 days;The activity of SOD and POD decreased gradually with the increase of artificial aging degree.The activity of SOD and POD decreased by 44.49% and 32.62% after aging for 7 days,respectively.The degree of SOD activity decreased was greater than that of POD activity(44.49%>32.62%) after aging treatment for the same time.During the aging process,the constitute of gliadin was changed.The color of gliadin band lightened on the third day and some gliadin bands disappeared in α,β,ω region with the increase of artificial aging degree.
引文
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[22] 吕燕燕.牧草种子劣变的机理及活力检测方法研究[D].兰州:兰州大学,2018.
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[27] 万里强,李向林,石永红,等.PEG 胁迫下4个黑麦草品种生理生化指标响应与比较研究[J].草业学报,2010,19(1):83-88.
[28] Jeng T L,Sung J M.Hydration effect on lipid peroxidation and peroxide scavenging enzyme activity ofartificiallyaged peanut seed[J].Seed Sci Techno,1994(22):531-539.
[29] Zhang M,Nakamaru Y,Tsuda S,et al.Enzymatic conver-sation of volatile metadolites in dry seeds during storage[J].Plant Cell Physio,1995,36(1):157-164.
[30] 史威威.七个品种燕麦种子发芽指标的主成分分析及良种选择[J].现代牧业,2018,2(3):17-20.
[31] Kalpanar,Madhava R K V.Lipid changes during accelerated aging of seeds of pigeon pea cultivars[J].Seed Science and Tech-nology,1996,24:275-285.
[32] 浦心春,韩建国,王培,等.高羊茅种子生活力丧失过程中遗传物质的降解[J].草地学报,1996,4(3):180-185.
[33] 孔治有,刘叶菊,覃鹏.40℃加速老化处理对小麦种子生理生化特性的影响[J].安徽农业科学,2010,38(12):6155-6157.
[34] 齐冰洁,刘景辉,高聚林,等.燕麦种质资源醇溶蛋白遗传多样性研究[J].麦类作物学报,2010,30(3):427-430.
[35] Woychick J H,Boundy J A,DimlerR J.Starch gel electrophoresis of wheat gluten proteins with concentric area[J].Arch Biochem Biophy,1961,94(3):477-482.
[36] 谭富娟,范传珠,马缘生,等.燕麦种子贮存后遗传完整性研究[J].种子,1997,19(5):9-12.
[2] 郭本兆,王世金,李健华.大麦属的属间亲缘和属下分类的研究[J].西北植物学报,1986,6(1):23-31.
[3] Megulre,Dvorak J.High salt tolerance potential in wheat grasses [J].Crop Science,1981,21:102-105.
[4] 吴勤.老芒麦改良干草原草地的效果分析[J].宁夏农林科技,1992(4):35-36.
[5] 王欣欣,卢萍,李鸿雁,等.种子老化影响老芒麦种子醇溶蛋白遗传完整性的研究[J].种子,2016,35(5):13-17.
[6] 韩亚琼,靳慧卿,贾振宇,等.种子老化对农牧老芒麦生理生化特性的影响[J].内蒙古农业大学学报(自然科学版),2017,38(5):22-29.
[7] H ampton J G,Tekrony D M.Handbook of vigour test methods [M].Handbook of vigour teat methods,1995.
[8] 刘霞,刘菲.蓝萼香茶菜种子人工老化过程中生理生化特性的研究[J].种子,2012,31(4):27-30.
[9] 孙春青,杨伟,戴忠良,等.人工老化处理对结球甘蓝种子生理生化特性的影响[J].西北植物学报,2012,32(8):1615-1620.
[10] 曾钦薇,谢永俊.人工老化诱导的油菜种子活力和抗氧化酶活性变化的研究[J].云南农业科技,2012(2):11-13.
[11] 吴浩,周青平,颜红波,等.垂穗披碱草种子老化过程中生理生化特性的研究[J].青海大学学报(自然科学版),2014(3):6-10.
[12] 吴浩.三种披碱草种子的生理生化特性及基因组对老化过程和响应[D].西宁:青海大学,2014.
[13] 周国栋.种子老化对老芒麦种质生理特性及遗传完整性变化的影响[D].北京:中国农业科学院,2012.
[14] 张自阳,马景周,王智煜,等.人工老化对小麦种子活力及醇溶蛋白组成的影响[J].种子,2017,36(8):42-47.
[15] 杨剑平,刘春辉,王海涛,等.人工老化处理对小麦种胚及幼苗生长的影响[J].北京农学院学报,1995(1):17-21.
[16] 梁海荣,邢侗姬,周小梅.不同活力的小麦种子生理生化及其修复的研究[J].山西大学学报(自然科学版),2010,33(2):286-290.
[17] 马跃青,张雷,吴卫国,等.人工加速老化对茶叶籽储藏特性的影响[J].中国粮油学报,2012,28(6):61-65.
[18] 王芳,卢新雄,马晓岗,等.基于醇溶蛋白的20份大麦种质资源遗传完整性分析[J].麦类作物学报,2007,27(4):607-612.
[19] 任守杰,张志娥,陈晓玲,等.基于醇溶蛋白的20 份小麦种质遗传完整性分析[J].植物遗传资源学报,2006,7(1):44-48.
[20] 李合生.植物生理生化实验原理与技术[M].北京:高等教育出版社,2002:192-194.
[21] 王彦荣,刘友良,沈益新.种子劣变的生理学研究进展综述[J].草地学报,2001,9(3):159-164.
[22] 吕燕燕.牧草种子劣变的机理及活力检测方法研究[D].兰州:兰州大学,2018.
[23] 周小梅,王自霞,乔燕祥.人工老化处理对芝麻种子生理生化特性的影响[J].中国油料作物学报,2008,30(4):460-463.
[24] 曹威.大麦醇溶蛋白的提取、性质及成膜研究[D].武汉:武汉轻工大学,2015.
[25] ISTA.国际种子检验规程[M].北京:中国农业出版社,1996:187-188.
[26] 顾炳朝,岳绪国,杨军,等.油菜种子老化处理后的生理生化变化[J].福建农业学报,2013,28(4):339-342.
[27] 万里强,李向林,石永红,等.PEG 胁迫下4个黑麦草品种生理生化指标响应与比较研究[J].草业学报,2010,19(1):83-88.
[28] Jeng T L,Sung J M.Hydration effect on lipid peroxidation and peroxide scavenging enzyme activity ofartificiallyaged peanut seed[J].Seed Sci Techno,1994(22):531-539.
[29] Zhang M,Nakamaru Y,Tsuda S,et al.Enzymatic conver-sation of volatile metadolites in dry seeds during storage[J].Plant Cell Physio,1995,36(1):157-164.
[30] 史威威.七个品种燕麦种子发芽指标的主成分分析及良种选择[J].现代牧业,2018,2(3):17-20.
[31] Kalpanar,Madhava R K V.Lipid changes during accelerated aging of seeds of pigeon pea cultivars[J].Seed Science and Tech-nology,1996,24:275-285.
[32] 浦心春,韩建国,王培,等.高羊茅种子生活力丧失过程中遗传物质的降解[J].草地学报,1996,4(3):180-185.
[33] 孔治有,刘叶菊,覃鹏.40℃加速老化处理对小麦种子生理生化特性的影响[J].安徽农业科学,2010,38(12):6155-6157.
[34] 齐冰洁,刘景辉,高聚林,等.燕麦种质资源醇溶蛋白遗传多样性研究[J].麦类作物学报,2010,30(3):427-430.
[35] Woychick J H,Boundy J A,DimlerR J.Starch gel electrophoresis of wheat gluten proteins with concentric area[J].Arch Biochem Biophy,1961,94(3):477-482.
[36] 谭富娟,范传珠,马缘生,等.燕麦种子贮存后遗传完整性研究[J].种子,1997,19(5):9-12.