摘要
目的运用激光多普勒技术观察耳神门穴埋针24小时对脑缺血的治疗效果。方法用50%FeCl_3化学诱导制造大脑中动脉血栓闭塞模型,耳针组采用锨针针刺耳神门,假针组将祛除针的锨针片胶布固定在神门穴上,药物组阿司匹林灌胃60 mg/kg一次,利用经颅激光多普勒检测缺血区脑血流,24小时后采用行为学评分测定神经运动功能,TTC技术测定脑梗死体积。结果50%FeCl_3贴敷后,脑血流迅速下降,20分钟下降至22%,脑血栓闭塞模型制作成功。与模型组比较,针刺耳神门穴20分钟可显著提高脑血流量(174%),改善脑缺血后神经运动功能,减少脑梗死体积(28.3%),P<0.01。阿司匹林和假针亦有效,假针效果最差。结论针刺耳神门穴治疗急性脑卒中,能迅速提高脑缺血后脑血流量,从而改善神经运动功能,减少梗死体积,效果优于药物和假针。
Objective To observe the effect of needle embedment on auricular Shenmen( TF4) 24 hours on cerebral blood flow and Infarction area by laser doppler technique. Methods 50% FeCl_3 was used to induce the thrombosis occlusion model of middle cerebral artery. Needle embedment( 24 hours)was used to auricular Shenmen( TF4) in auricular needle group. Circle slice without needle was fixed on TF4 in sham-auricular needle group. Aspirin( 60 mg/kg) was intragastric administration in drug group once a day. Regional cerebral blood flow( r CBF) was measured with Laser Doppler flowmeter. Nerve motor function was measured with Neurological deficit scores. Infarction area was measured by 2,3,5-triphenyltetrazolium chloride staining method. Results After 50% FeCl_3 application,the cerebral blood flow decreased rapidly and decreased to 22% by 20 minutes. The model of cerebral thrombosis occlusion was successful. Compared with the model group,the r CBF in auricular needle group was raised to 174% in 20 min. Needle embedment on TF4 can improve the nerve motor function and reduce the infarction volume( 34%),P < 0. 01. Aspirin and sham-auricular were also effective. The effect of sham-auricular was the worst. Conclusion Needle embedment on auricular TF4 can rapidly improve the cerebral blood flow after cerebral ischemia,thereby improving the nerve motor function and reducing the infarct volume,and the effect is better than oral aspirin and sham-auricular needle.
引文
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