针刺耳神门穴对脑血栓大鼠脑血流的影响
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摘要
目的运用激光多普勒技术观察耳神门穴埋针24小时对脑缺血的治疗效果。方法用50%FeCl_3化学诱导制造大脑中动脉血栓闭塞模型,耳针组采用锨针针刺耳神门,假针组将祛除针的锨针片胶布固定在神门穴上,药物组阿司匹林灌胃60 mg/kg一次,利用经颅激光多普勒检测缺血区脑血流,24小时后采用行为学评分测定神经运动功能,TTC技术测定脑梗死体积。结果50%FeCl_3贴敷后,脑血流迅速下降,20分钟下降至22%,脑血栓闭塞模型制作成功。与模型组比较,针刺耳神门穴20分钟可显著提高脑血流量(174%),改善脑缺血后神经运动功能,减少脑梗死体积(28.3%),P<0.01。阿司匹林和假针亦有效,假针效果最差。结论针刺耳神门穴治疗急性脑卒中,能迅速提高脑缺血后脑血流量,从而改善神经运动功能,减少梗死体积,效果优于药物和假针。
Objective To observe the effect of needle embedment on auricular Shenmen( TF4) 24 hours on cerebral blood flow and Infarction area by laser doppler technique. Methods 50% FeCl_3 was used to induce the thrombosis occlusion model of middle cerebral artery. Needle embedment( 24 hours)was used to auricular Shenmen( TF4) in auricular needle group. Circle slice without needle was fixed on TF4 in sham-auricular needle group. Aspirin( 60 mg/kg) was intragastric administration in drug group once a day. Regional cerebral blood flow( r CBF) was measured with Laser Doppler flowmeter. Nerve motor function was measured with Neurological deficit scores. Infarction area was measured by 2,3,5-triphenyltetrazolium chloride staining method. Results After 50% FeCl_3 application,the cerebral blood flow decreased rapidly and decreased to 22% by 20 minutes. The model of cerebral thrombosis occlusion was successful. Compared with the model group,the r CBF in auricular needle group was raised to 174% in 20 min. Needle embedment on TF4 can improve the nerve motor function and reduce the infarction volume( 34%),P < 0. 01. Aspirin and sham-auricular were also effective. The effect of sham-auricular was the worst. Conclusion Needle embedment on auricular TF4 can rapidly improve the cerebral blood flow after cerebral ischemia,thereby improving the nerve motor function and reducing the infarct volume,and the effect is better than oral aspirin and sham-auricular needle.
Objective To observe the effect of needle embedment on auricular Shenmen( TF4) 24 hours on cerebral blood flow and Infarction area by laser doppler technique. Methods 50% FeCl_3 was used to induce the thrombosis occlusion model of middle cerebral artery. Needle embedment( 24 hours)was used to auricular Shenmen( TF4) in auricular needle group. Circle slice without needle was fixed on TF4 in sham-auricular needle group. Aspirin( 60 mg/kg) was intragastric administration in drug group once a day. Regional cerebral blood flow( r CBF) was measured with Laser Doppler flowmeter. Nerve motor function was measured with Neurological deficit scores. Infarction area was measured by 2,3,5-triphenyltetrazolium chloride staining method. Results After 50% FeCl_3 application,the cerebral blood flow decreased rapidly and decreased to 22% by 20 minutes. The model of cerebral thrombosis occlusion was successful. Compared with the model group,the r CBF in auricular needle group was raised to 174% in 20 min. Needle embedment on TF4 can improve the nerve motor function and reduce the infarction volume( 34%),P < 0. 01. Aspirin and sham-auricular were also effective. The effect of sham-auricular was the worst. Conclusion Needle embedment on auricular TF4 can rapidly improve the cerebral blood flow after cerebral ischemia,thereby improving the nerve motor function and reducing the infarct volume,and the effect is better than oral aspirin and sham-auricular needle.
引文
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[13]陈峰,杨易平,盛燮荪,等.针刺耳神门穴治疗脑动脉硬化症的临床研究[J].中医杂志,1999,40(10):344-356.
[14]雷茹,王颖.缺血性中风的针灸疗法研究进展[J].黑龙江中医药,2015,44(1):63-64.
[15]陈峰,戴晴,杨易平,等.耳神门穴对脑动脉硬化症患者椎一基底动脉血流的即时作用观察[J].中国针灸,1997,17(12):717-718.
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[18]Karatas H,Erdener SE,Gursoy-Ozdemir Y,et al.Thrombotic distal middle cerebral artery occlusion produced by topical Fe Cl3application:a novel model suitable for intravital microscopy and thrombolysis studies[J].Journal of Cerebral Blood Flow&Metabolism,J Cereb Blood Flow Metab,2011,31(6):1452-1460
[2]杨卉.耳针疗法作用机理的研究进展[J].湖北中医药大学学报,2011,13(2):65-67.
[3]张颖清.全息生物学-驳邹承鲁院士-爱国主义与诺贝尔奖[J].太原师范学院学报(社会科学版),2007,6(6):15-19.
[4]邢宏义,关新民.针刺治疗脑缺血的机理研究述评[J].中国针灸,1996,16(5):56-58.
[5]赵百孝,林岷瑜.法国耳穴疗法[C]//2011中国针灸学会年会论文集(摘要),2011:2.
[6]卢雨微,付宗英.耳针对血管性痴呆大鼠行为学及大脑皮质STAT1表达的影响[J].内蒙古中医药,2014,33(10):100-101.
[7]舒遵华,王国强,黎明全,等.耳针治疗椎-基底动脉供血不足疗效观察[J].中国针灸,2014,34(12):1161-1164.
[8]孙晶.针灸治疗脑缺血再灌注损伤炎症反应的研究进展[C]//浙江大学,嘉兴学院.2012年浙江省针灸学会年会暨学术交流会论文汇编,2012:6.
[9]史敏.针灸对脑缺血保护作用机制综述[J].内蒙古中医药,2014,33(10):105.
[10]朱靓贤,王彩虹.耳穴贴压应用于脑卒中可干预危险因素的临床研究进展[J].上海针灸杂志,2010,29(2):127-130.
[11]叶明柱,冯禾昌.试论耳穴“神门”[J].中国针灸,2002,22(增刊):208-210.
[12]包玉龙,朱竟赫,范英兰,等.线栓法与改良三氯化铁法致大鼠大脑中动脉闭塞模型的对比研究[J].动物医学进展,2014,35(5):65-70.
[13]陈峰,杨易平,盛燮荪,等.针刺耳神门穴治疗脑动脉硬化症的临床研究[J].中医杂志,1999,40(10):344-356.
[14]雷茹,王颖.缺血性中风的针灸疗法研究进展[J].黑龙江中医药,2015,44(1):63-64.
[15]陈峰,戴晴,杨易平,等.耳神门穴对脑动脉硬化症患者椎一基底动脉血流的即时作用观察[J].中国针灸,1997,17(12):717-718.
[16]窦如海.动物的耳廓与耳针穴位[M].济南:实验动物与动物实验技术,1992:81-84.
[17]陶静,邹愉龙,洪江从,等.脑卒中大鼠模型的行为学评测方法概述[J].亚太传统医药,2013,9(3):73-74.
[18]Karatas H,Erdener SE,Gursoy-Ozdemir Y,et al.Thrombotic distal middle cerebral artery occlusion produced by topical Fe Cl3application:a novel model suitable for intravital microscopy and thrombolysis studies[J].Journal of Cerebral Blood Flow&Metabolism,J Cereb Blood Flow Metab,2011,31(6):1452-1460