基于电磁力检测细胞粘附力的方法与检测仪器
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摘要
开发一套测量细胞间、细胞与基质分子间粘附力的仪器。根据磁场力学的原理,由DA(数模)转换模块控制电磁铁电流的大小从而改变磁场,结合麦克斯韦简化公式,磁场对处理过的膜内含有纳米磁粉的细胞具有吸引力的作用,当细胞的粘附力和磁场的作用力达到平衡的时候,通过测量磁场的吸引力就能间接测出细胞间的粘附力大小。结合电子电路软硬件的设计完成了电磁力吸引测量细胞粘附力仪器的开发,并通过Maxwell软件对仪器核心部件——吸盘式电磁铁进行磁场的仿真分析,得到磁场仿真数据,与仪器测试数据进行对照分析,发现这两组数据在所通电流大小和磁力大小的关系趋势上非常吻合。开发了一种细胞粘附力检测仪器,为测量粘附力提供了一种新的方法,通过改变电流大小从而改变磁场大小,得到相应的细胞粘附力大小,具有无接触式测量、不会对细胞造成损伤等优点,该技术具有一定的实用性。
To develop an instrument for measuring the adhesion between cells or cell and matrix molecules. According to the principle of magnetic field mechanics,the DA(digital-to-analog)conversion module controls the current of the magnitude through the electromagnet to change the magnetic field. Combined with the simplified formula of Maxwell,the magnetic field has an attractive effect on the cells containing the nano-magnetic powder in the treated membrane. When the adhesion and the force of the magnetic field reach equilibrium,the adhesion between the cells can be indirectly measured by measuring the attraction force of the magnetic field. In combination with the design of electronic circuit hardware and software,the development of electromagnetic attraction cell adhesion detecting instrument was completed. The simulation of the surrounding magnetic field on the core component of the apparatus was carried out by Maxwell software,and the magnetic field simulation data was obtained. Compared with the data obtained from the instrument test,it can be found that the two sets of data are very similar in the relationship between the magnitude of the current and the magnitude of the magnetic force. It has developed a cell adhesion detection instrument that provides a new method for measuring adhesion by changing the scope of the current to change the size of the magnetic field,resulting in a corresponding cell adhesion,without contact measurement and damage to cells. This technique has certain practicability.
To develop an instrument for measuring the adhesion between cells or cell and matrix molecules. According to the principle of magnetic field mechanics,the DA(digital-to-analog)conversion module controls the current of the magnitude through the electromagnet to change the magnetic field. Combined with the simplified formula of Maxwell,the magnetic field has an attractive effect on the cells containing the nano-magnetic powder in the treated membrane. When the adhesion and the force of the magnetic field reach equilibrium,the adhesion between the cells can be indirectly measured by measuring the attraction force of the magnetic field. In combination with the design of electronic circuit hardware and software,the development of electromagnetic attraction cell adhesion detecting instrument was completed. The simulation of the surrounding magnetic field on the core component of the apparatus was carried out by Maxwell software,and the magnetic field simulation data was obtained. Compared with the data obtained from the instrument test,it can be found that the two sets of data are very similar in the relationship between the magnitude of the current and the magnitude of the magnetic force. It has developed a cell adhesion detection instrument that provides a new method for measuring adhesion by changing the scope of the current to change the size of the magnetic field,resulting in a corresponding cell adhesion,without contact measurement and damage to cells. This technique has certain practicability.
引文
[1] 滕维中.几类细胞的生物力学实验研究[D].太原:太原理工大学,2005.
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[6] Lee L M,Liu A P.A Microfluidic Pipette Array for Mechanophenotyping of Cancer Cells and Mechanical Gating of Mechanosensitive Channels[J].Lab on a Chip,2015,15(1):264-273.
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[10] Friedrichs J,Helenius J,Muller D J.Quantifying Cellular Adhesion to Extracellular Matrix Components by Single-Cell Force Spectroscopy[J].Nat Protoc,2010,5(7):1353-1361.
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[12] 陈昇.基于纳米机器人的细胞粘附力的定量研究[D].哈尔滨:哈尔滨工业大学,2017.
[13] 周瑞雪,王海燕,朱德斌,等.光镊技术在生物学中的应用新进展[J].激光生物学报,2017,4(5):289-293.
[14] 李同合.电磁继电器的“组合”设计几种形式[J].物理教师,2012(4):30-30.
[15] Kawase Y,Ito S.Analysis of Attractive Force of Pull-Typesingle Phase AC Electromagnets[J].IEEE,1990,26(2):1046-1049.
[16] 宋俊,梁国星,张宏,等.平行对极电磁铁磁场分布特性实验与研究[J].科学技术与工程,2014(2):27-31.
[17] 陈华良.磁性纳米粒子的制备和包覆[J].广东化工,2016,16(3):252-254.
[18] 王晶,徐玉清,王文秀.磁性纳米粒子及其在生物医学中的应用进展[J].药物生物技术,2007,14(5):386-390.
[19] Marago O M,Jones P H,Gucciardi P G,et al.Optical Trapping and Manipulation of Nanostructures[J].Nature Nanotechnology,2013,8(11):807-819.
[20] 王巧英,祝青,周丽蓉,等.磁性氧化铁纳米粒子在生物医学应用中的研究进展[J].山西医药杂志,2016(3):272-275.
[21] 邱星屏.四氧化三铁磁性纳米粒子的合成及表征[J].厦门大学学报(自然版),1999,38(5):711-715.应梦迪(1995-),女,浙江安吉人,硕士研究生。现于杭州电子科技大学生物医学工程专业攻读硕士学位,主要研究方向为生物力学与反应器,191030965@qq.com;李宏(1966-),男,教授(高级工程师),硕士生导师。主要研究方向为组织工程与生物反应器,生物力学与医学仪器开发,lihong@hdu.edu.cn。
[2] Rebelo L M,De Sousa J S,Mendes Filho J,et al.Comparison of the Viscoelastic Properties of Cells from Different Kidney Cancer Phenotypes Measured with Atomic Force Microscopy[J].Nanotechnology,2013,5(5):1-12.
[3] 于淼.基于原子力显微镜的细胞若干力学特性的定量研究[D].哈尔滨:哈尔滨工业大学,2015.
[4] Fu Jianping,Wang Yangkao,Yang Michael,et al.Mechanical Regulation of Cell Function with Geometrically Modulated Elastomeric Substrates[J].Nature Methods,2010,7(9):733-736.
[5] Gilbert P M,Havenstrite K L,Magnusson K E G,et al.Substrate Elasticity Regulates Skeletal Muscle Stem Cell Self-Renewal in Culture[J].Science,2010,329(5995):1078-1081.
[6] Lee L M,Liu A P.A Microfluidic Pipette Array for Mechanophenotyping of Cancer Cells and Mechanical Gating of Mechanosensitive Channels[J].Lab on a Chip,2015,15(1):264-273.
[7] 徐文奎.基于压阻式悬臂梁传感器对细胞力学特性的研究[D].苏州:苏州大学,2016.
[8] 李淑萍.基于AFM的细胞力学特性实验研究[D].哈尔滨:哈尔滨工业大学,2014.
[9] Helenius J,Heisenberg C P,Gaub H E,et al.Single-Cellforce Spectroscopy[J].Journal of Cell Science,2008,121(11):1785-1791.
[10] Friedrichs J,Helenius J,Muller D J.Quantifying Cellular Adhesion to Extracellular Matrix Components by Single-Cell Force Spectroscopy[J].Nat Protoc,2010,5(7):1353-1361.
[11] Neuman K C,Nagy A.Single-Molecule Force Spectroscopy:Optical Tweezers,Magnetic Tweezers and Atomic Force Microscopy[J].Nat Methods,2008,5(6):491-505.
[12] 陈昇.基于纳米机器人的细胞粘附力的定量研究[D].哈尔滨:哈尔滨工业大学,2017.
[13] 周瑞雪,王海燕,朱德斌,等.光镊技术在生物学中的应用新进展[J].激光生物学报,2017,4(5):289-293.
[14] 李同合.电磁继电器的“组合”设计几种形式[J].物理教师,2012(4):30-30.
[15] Kawase Y,Ito S.Analysis of Attractive Force of Pull-Typesingle Phase AC Electromagnets[J].IEEE,1990,26(2):1046-1049.
[16] 宋俊,梁国星,张宏,等.平行对极电磁铁磁场分布特性实验与研究[J].科学技术与工程,2014(2):27-31.
[17] 陈华良.磁性纳米粒子的制备和包覆[J].广东化工,2016,16(3):252-254.
[18] 王晶,徐玉清,王文秀.磁性纳米粒子及其在生物医学中的应用进展[J].药物生物技术,2007,14(5):386-390.
[19] Marago O M,Jones P H,Gucciardi P G,et al.Optical Trapping and Manipulation of Nanostructures[J].Nature Nanotechnology,2013,8(11):807-819.
[20] 王巧英,祝青,周丽蓉,等.磁性氧化铁纳米粒子在生物医学应用中的研究进展[J].山西医药杂志,2016(3):272-275.
[21] 邱星屏.四氧化三铁磁性纳米粒子的合成及表征[J].厦门大学学报(自然版),1999,38(5):711-715.应梦迪(1995-),女,浙江安吉人,硕士研究生。现于杭州电子科技大学生物医学工程专业攻读硕士学位,主要研究方向为生物力学与反应器,191030965@qq.com;李宏(1966-),男,教授(高级工程师),硕士生导师。主要研究方向为组织工程与生物反应器,生物力学与医学仪器开发,lihong@hdu.edu.cn。