摘要
目的建立长角血蜱野外种群对常用杀虫剂的相对敏感基线和诊断剂量。方法分别于2013年和2014年4-9月采集河南省王屋山区未接触过任何杀虫剂的长角血蜱野外种群。采用药膜法测定长角血蜱成蜱对杀虫剂的敏感性。采用DPS数据处理软件进行机值分析,拟合毒力回归方程,确定半数致死剂量、99%致死剂量,计算诊断剂量。结果建立了对敌敌畏、双硫磷、仲丁威、氯菊酯、溴氰菊酯和高效氯氰菊酯6种杀虫剂的相对敏感基线,分别为y=1.549 9+1.359 4x、y=2.343 3+1.225 6x、y=-6.850 7+5.213 9x、y=0.866 1+1.571 1x、y=0.411 6+2.277 8x和y=2.551 8+1.018 9x;相应的诊断剂量分别为35.505 6、10.764 7、1.047 4、25.872 9、2.171 4和97.063 2 mg/L。结论建立了长角血蜱野外种群对常用杀虫剂的相对敏感基线和诊断剂量,可作为长角血蜱抗药性监测的参考。
Objective To establish the relative susceptibility baselines and diagnostic doses of the wild population of Haemaphysalis longicornis to common insecticides. Methods The wild population of H. longicornis without exposure to any pesticides was collected at Mount Wangwu in Henan, China from April to September in both 2013 and 2014. The residual film method was used to determine the susceptibility of adult H. longicornis to insecticides. DPS data processing software was used for probit analysis, and the toxicity regression equation was fitted to determine the median lethal dose and99% lethal dose and then to calculate the diagnostic dose. Results The susceptibility baselines of H. longicornis to dichlorvos,temephos, bassa, permethrin, deltamethrin, and beta-cypermethrin were y= 1.549 9+ 1.359 4 x, y=2.343 3+ 1.225 6 x,y=-6.850 7 + 5.213 9 x, y=0.866 1 + 1.571 1 x, y=0.411 6 + 2.277 8 x, and y=2.551 8+ 1.018 9 x, respectively; the diagnostic doses were 35.505 6, 10.764 7, 1.047 4, 25.872 9, 2.171 4, and 97.063 2 mg/L, respectively. Conclusion The relative susceptibility baselines of the wild population of H. longicornis to the six insecticides, as well as the diagnostic doses, have been established. The results can be used as a reference for monitoring the resistance of H. longicornis to pesticides.
引文
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