摘要
以离子液体为提取剂,采用离子液体超声波辅助提取黑豆皮花青素,考察了提取温度、提取时间、离子液体浓度、料液比对黑豆皮花青素得率的影响,采用响应曲面法对提取工艺进行优化,同时对花青素的体外抗氧化活性进行研究。结果表明,最佳提取条件:离子液体浓度0.9 mol/L,料液比1∶53(g∶m L)、提取温度43℃、提取时间45 min。在此条件下黑豆皮花青素的得率4.12 mg/g。花青素对羟自由基的清除效果在一定范围内优于维生素C。
Using ionic liquid as extraction solvent, anthocyanin was extracted from black soybean peel by ultrasonic assisted extraction. The effects of extraction temperature, time, ionic liquid concentration, material-liquid ratio on anthocyanin yield were investigated. The extraction technology was optimized by response surface methodology. The in vitro antioxidant activity of anthocyanin from black soybean peel was researched. The results showed that the optimum conditions were as follows: ionic liquid concentration 0.9 mol/L, material-liquid ratio 1∶53(g∶ml), extraction temperature 43 ℃,time 45 min. Under the optimal conditions, the anthocyanin yield from the black soybean peel was 4.12 mg/g. The scavenging effect of anthocyanin on hydroxyl radicals was better than that of Vitamin C in certain range.
引文
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