利用SNP芯片解析油菜杂交种丰油10号的遗传基础
|
摘要
为解析高产稳产油菜品种丰油10号的分子遗传学基础,利用油菜60K SNP芯片对丰油10号及包括其2个亲本在内的20份骨干亲本系进行了基因型分析。结果表明:20份骨干亲本系间的遗传距离变幅为0.123～0.463,平均为0.348。丰油10号母本22A和父本P087-2间遗传距离为0.375,高于平均遗传距离。聚类分析将20份骨干亲本系划分为4个类群,其中母本22A归在类群Ⅰ,父本P087-2归在类群Ⅳ。基因组比较发现杂交种丰油10号与母本22A的相似性比例为64.77%,与父本P087-2的相似性比例为62.89%,其中有30.78%的基因组杂合区段来源于双亲不同基因型间的组合。同时发现丰油10号与父母本在19条染色体上的多态性比例存在比较大的差异。该研究为后续油菜品种的选育提供理论指导。
In order to analyze the molecular genetic basis of rape hybrid Fengyou No.10,Fengyou No.10 and 20 elite inbred lines including its parents were genotyped using the Illumina 60 K SNP chip technology.The results showed that the genetic distance between pairs of 20 elite inbred lines ranged from 0.123 to 0.463,with an average of 0.348.The genetic distance between the female parent 22 A and male parent P087-2 of Fengyou No.10 was 0.375,which was higher than the average value of 20 elite inbred lines.All these inbred lines were divided into four groups using cluster analysis,in which the female parent 22 A and male parent P087-2 belong to the first group and the fourth group,respectively.Comparative genome analysis found that the hybrid Fengyou No.10 had the similarity of 64.77% and62.89% with its female parent 22 A and male parent P087-2,respectively.The 30.78% loci of Fengyou No.10 were heterozygous.At the same time,the great differences of the polymorphism rate in 19 chromosomes were observed between Fengyou No.10 and the parents.Our study would provide a theoretical guide for subsequent variety breeding in Brassica napus.
In order to analyze the molecular genetic basis of rape hybrid Fengyou No.10,Fengyou No.10 and 20 elite inbred lines including its parents were genotyped using the Illumina 60 K SNP chip technology.The results showed that the genetic distance between pairs of 20 elite inbred lines ranged from 0.123 to 0.463,with an average of 0.348.The genetic distance between the female parent 22 A and male parent P087-2 of Fengyou No.10 was 0.375,which was higher than the average value of 20 elite inbred lines.All these inbred lines were divided into four groups using cluster analysis,in which the female parent 22 A and male parent P087-2 belong to the first group and the fourth group,respectively.Comparative genome analysis found that the hybrid Fengyou No.10 had the similarity of 64.77% and62.89% with its female parent 22 A and male parent P087-2,respectively.The 30.78% loci of Fengyou No.10 were heterozygous.At the same time,the great differences of the polymorphism rate in 19 chromosomes were observed between Fengyou No.10 and the parents.Our study would provide a theoretical guide for subsequent variety breeding in Brassica napus.
引文
[1]Liu S,Fan C C,Li J N,et al.A genome-wide association study reveals novel elite allelic variations in seed oil content of Brassica napus.Theoretical and Applied Genetics,2016,129(6):1203-1215.
[2]农业部种植业管理司.全国大宗油料作物生产发展规划(2016~2020年).中国农业信息,2017(1):6-15.
[3]张毅.我国油菜生产及油料需求分析.农业技术与装备,2009(13):14-16.
[4]郭海洋.基于HRM技术的水稻全基因组SNP标记开发及其初步应用.广州:华南农业大学,2016.
[5]Yan J B,Yang X H,Shah T,et al.High-throughput SNP genotyping with the GoldenGate assay in maize.Molecular Breeding,2010,25(3):441-451.
[6]Khan M A,Han Y,Zhao Y F,et al.A high-throughput apple SNPgenotyping platform using the GoldenGateTM assay.Gene,2012,494(2):196-201.
[7]Wang N,Li F,Chen B,et al.Genome-wide investigation of genetic changes during modern breeding of Brassica napus.Theoretical and Applied Genetics,2014,127(8):1817-1829.
[8]桑世飞,王会,梅德圣,等.利用全基因组SNP芯片分析油菜遗传距离与杂种优势的关系.中国农业科学,2015,48(12):2469-2478.
[9]于海至.中国油菜强优势杂交种骨干亲本的遗传分析.武汉:华中农业大学,2015.
[10]Liu L Z,Qu C M,Wittkop B J,et al.A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L..PLoSONE,2013,8(12):e83052.
[11]Wang X,Yu K,Li H,et al.High-density SNP map construction and QTL identification for the apetalous character in Brassica napus L..Frontiers in Plant Science,2015,6(105):1164-1178.
[12]卢坤,王腾岳,徐新福,等.甘蓝型油菜结角高度与荚层厚度的全基因组关联分析.作物学报,2016,42(3):344-352.
[13]Tan M,Liao F,Hou L,et al.Genome-wide association analysis of seed germination percentage and germination index in Brassica napus L.under salt and drought stresses.Euphytica,2017,213(2):40-54.
[14]张书芬.国审高产双低油菜杂交种丰油10号.种业导刊,2005(6):44.
[15]朱家成,张书芬,王建平,等.高产优质甘蓝型油菜杂交种丰油10号的选育.中国种业,2011(11):56-57.
[16]曹金华,朱家成,张书芬,等.覆盖对土壤温度及甘蓝型油菜丰油10号抗寒性和产量的影响.中国油料作物学报,2014,36(2):213-218.
[17]王子敏,乐明凯.丰油9号和丰油10号迟播冬发高产技术.中国种业,2008(11):56.
[18]Liu K J,Muse S V.PowerMarker:an integrated analysis environment for genetic marker analysis.Bioinformatics,2005,21(9):2128-2129.
[19]Kumar S,Stecher G,Tamura K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets.Molecular Biology and Evolution,2016,33:1870-1874.
[20]Wickham H.ggplot2:Elegant Graphics for Data Analysis.SpringerVerlag New York,2016.
[21]路明,张志军,郑淑波,等.基于SNP标记分析玉米品种吉单50的遗传基础.玉米科学,2016,24(6):1-7.
[22]Riaz A,Li G,Quresh Z,et al.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance.Plant Breeding,2001,120:411-415.
[23]沈金雄,陆光远,傅廷栋,等.甘蓝型油菜遗传多样性及其与杂种表现的关系.作物学报,2002,28(5):622-627.
[24]徐小栋,陈飞,倪西源,等.甘蓝型油菜分子距离与杂种优势和产量表型的相关分析.浙江大学学报(农业与生命科学版),2010,36(6):641-649.
[25]王凯华,张文,王会,等.国内外甘蓝型油菜种质SSR标记遗传多样性分析.中国农学通报,2011,27(19):144-149.
[26]宋立红,智文良,李玮,等.我国近年审定的甘蓝型油菜品种的遗传多样性分析.西南农林科技大学学报,2011,39(11):110-118.
[27]何俊平,张书芬,王建平,等.甘蓝型油菜育种亲本材料遗传多样性的SSR分析.西南农业学报,2015,28(6):2374-2380.
[2]农业部种植业管理司.全国大宗油料作物生产发展规划(2016~2020年).中国农业信息,2017(1):6-15.
[3]张毅.我国油菜生产及油料需求分析.农业技术与装备,2009(13):14-16.
[4]郭海洋.基于HRM技术的水稻全基因组SNP标记开发及其初步应用.广州:华南农业大学,2016.
[5]Yan J B,Yang X H,Shah T,et al.High-throughput SNP genotyping with the GoldenGate assay in maize.Molecular Breeding,2010,25(3):441-451.
[6]Khan M A,Han Y,Zhao Y F,et al.A high-throughput apple SNPgenotyping platform using the GoldenGateTM assay.Gene,2012,494(2):196-201.
[7]Wang N,Li F,Chen B,et al.Genome-wide investigation of genetic changes during modern breeding of Brassica napus.Theoretical and Applied Genetics,2014,127(8):1817-1829.
[8]桑世飞,王会,梅德圣,等.利用全基因组SNP芯片分析油菜遗传距离与杂种优势的关系.中国农业科学,2015,48(12):2469-2478.
[9]于海至.中国油菜强优势杂交种骨干亲本的遗传分析.武汉:华中农业大学,2015.
[10]Liu L Z,Qu C M,Wittkop B J,et al.A high-density SNP map for accurate mapping of seed fibre QTL in Brassica napus L..PLoSONE,2013,8(12):e83052.
[11]Wang X,Yu K,Li H,et al.High-density SNP map construction and QTL identification for the apetalous character in Brassica napus L..Frontiers in Plant Science,2015,6(105):1164-1178.
[12]卢坤,王腾岳,徐新福,等.甘蓝型油菜结角高度与荚层厚度的全基因组关联分析.作物学报,2016,42(3):344-352.
[13]Tan M,Liao F,Hou L,et al.Genome-wide association analysis of seed germination percentage and germination index in Brassica napus L.under salt and drought stresses.Euphytica,2017,213(2):40-54.
[14]张书芬.国审高产双低油菜杂交种丰油10号.种业导刊,2005(6):44.
[15]朱家成,张书芬,王建平,等.高产优质甘蓝型油菜杂交种丰油10号的选育.中国种业,2011(11):56-57.
[16]曹金华,朱家成,张书芬,等.覆盖对土壤温度及甘蓝型油菜丰油10号抗寒性和产量的影响.中国油料作物学报,2014,36(2):213-218.
[17]王子敏,乐明凯.丰油9号和丰油10号迟播冬发高产技术.中国种业,2008(11):56.
[18]Liu K J,Muse S V.PowerMarker:an integrated analysis environment for genetic marker analysis.Bioinformatics,2005,21(9):2128-2129.
[19]Kumar S,Stecher G,Tamura K.MEGA7:Molecular evolutionary genetics analysis version 7.0 for bigger datasets.Molecular Biology and Evolution,2016,33:1870-1874.
[20]Wickham H.ggplot2:Elegant Graphics for Data Analysis.SpringerVerlag New York,2016.
[21]路明,张志军,郑淑波,等.基于SNP标记分析玉米品种吉单50的遗传基础.玉米科学,2016,24(6):1-7.
[22]Riaz A,Li G,Quresh Z,et al.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance.Plant Breeding,2001,120:411-415.
[23]沈金雄,陆光远,傅廷栋,等.甘蓝型油菜遗传多样性及其与杂种表现的关系.作物学报,2002,28(5):622-627.
[24]徐小栋,陈飞,倪西源,等.甘蓝型油菜分子距离与杂种优势和产量表型的相关分析.浙江大学学报(农业与生命科学版),2010,36(6):641-649.
[25]王凯华,张文,王会,等.国内外甘蓝型油菜种质SSR标记遗传多样性分析.中国农学通报,2011,27(19):144-149.
[26]宋立红,智文良,李玮,等.我国近年审定的甘蓝型油菜品种的遗传多样性分析.西南农林科技大学学报,2011,39(11):110-118.
[27]何俊平,张书芬,王建平,等.甘蓝型油菜育种亲本材料遗传多样性的SSR分析.西南农业学报,2015,28(6):2374-2380.