牛蒡子苷元对骨肉瘤细胞增殖和侵袭影响研究
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摘要
目的:探讨牛蒡子苷元对骨肉瘤细胞增殖和侵袭影响。方法:采用人骨肉瘤MG-63细胞为研究对象,用不同浓度(0、10、20、40、80和100μmol/L)的牛蒡子苷元干预骨肉瘤细胞。采用MTT法测定细胞增殖能力、Transwell法测定细胞侵袭能力和流式细胞术检测各组细胞凋亡情况。结果:牛蒡子苷元能够抑制骨肉瘤细胞MG-63的增殖,降低肿瘤细胞侵袭性,促进凋亡,随着药物浓度的增加效果持续增加,具有统计学差异(P<0.05)。结论:牛蒡子苷元可抑制骨肉瘤细胞增殖和侵袭,诱导细胞发生凋亡,具有良好的抗肿瘤效果。
Objective:To investigate the effect of arctigenin on proliferation and invasion of osteosarcoma cells. Methods:Human osteosarcoma MG-63 cells were used as the study object,and arctigenin with different concentrations(0,10,20,40,80 and 100μmol/L) was used to intervene osteosarcoma cells. MTT assay was used to measure cell proliferation. Transwell assay was used to test cell invasion. And flow cytometry was used to detect cell apoptosis in each groups. Results:Arctigenin could inhibit the proliferation of MG-63 cells,reduce the invasion of tumor cells and promote cell apoptosis. With the increasing of arctigenin concentration,there was a significant effect with statistical difference(P<0.05). Conclusion:Arctigenin can inhibit the proliferation and invasion of osteosarcoma,promote apoptosis,and has well anti-tumor effect.
Objective:To investigate the effect of arctigenin on proliferation and invasion of osteosarcoma cells. Methods:Human osteosarcoma MG-63 cells were used as the study object,and arctigenin with different concentrations(0,10,20,40,80 and 100μmol/L) was used to intervene osteosarcoma cells. MTT assay was used to measure cell proliferation. Transwell assay was used to test cell invasion. And flow cytometry was used to detect cell apoptosis in each groups. Results:Arctigenin could inhibit the proliferation of MG-63 cells,reduce the invasion of tumor cells and promote cell apoptosis. With the increasing of arctigenin concentration,there was a significant effect with statistical difference(P<0.05). Conclusion:Arctigenin can inhibit the proliferation and invasion of osteosarcoma,promote apoptosis,and has well anti-tumor effect.
引文
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[16] 张晓娟,张燕丽,左冬冬,等.牛蒡子的质量控制、药理作用研究进展[J].中医药信息,2013,30(6):125-128.
[2] Kang K,Lee HJ,Yoo JH,et al.Cell and nuclear enlargement of SW480 cells induced by a plant lignan,arctigenin:evaluation of cellular DNA content using fluorescence microscopy and flow cytometry [J].DNA Cell Biol,2011,30 (8):623-629.
[3] Gu Y,Scheuer C,Feng D,et al. Inhibition of angiogenesis:a novel antitumor mechanism of the herbal compound arctigenin [J].Anti-cancer Drugs,2013,24(8):781-791.
[4] Susanti S,Iwasaki H,Inafuku M,et al. Mechanism of arctigenin-mediated specific cytotoxicity against human lung adenocarcinoma cell lines[J].Phytomedicine,2013,21(1):39-46.
[5] 张德绸,王廷帆,葛建华.骨肉瘤最新治疗及研究进展[J].西南医科大学学报,2018,41(5):473-476.
[6] 白月琴,刘宇,赵晓珍.基于微环境认识中医药抗肿瘤转移的机制[J].陕西中医,2016,37(10):1438-1439.
[7] 张甘霖,杨国旺,于明薇,等.自噬——中医药抗肿瘤研究的新方向[J].中华中医药杂志,2014,29(2):507-510.
[8] 王哲,王佳贺.牛蒡子苷元药理作用的研究进展[J].中国医药导报,2018,15(32):50-53.
[9] 马天宇,陈燕平,程素盼,等.牛蒡子研究进展[J].辽宁中医药大学学报,2018,20(9):113-116.
[10] 甄妍.巧用中药牛蒡子配伍发挥降血糖及抗肿瘤功效探讨[J].中国药物与临床,2018,18(8):1392-1393.
[11] 张兴德,张彩琴,刘启迪,等.牛蒡子抗肿瘤活性成分及作用机制研究进展[J].中国现代中药,2012,14(12):12-17.
[12] 鲍雯雯,历淑芬,丛晓东,等.牛蒡子药理作用和临床应用研究进展[J].中国民族民间医药,2011,20(3):45-46.
[13] 郑国灿,王兵,黄聪华,等.牛蒡子苷元对肿瘤血管生成抑制作用的观察[J].中华肿瘤预防杂志,2013,20(3):177-180.
[14] 孙铁民,梁伟,林莉,等.牛蒡子对癌瘤作用的实验研究[J].辽宁中医学院学报,2002,4(4):310.
[15] Jin SK,Hyung JJ,Jin BJ,et al.Induction of apoptosis and acetylation of histone H3 and H4 by arctigenin in the human melanoma cell line SK-MEL-28[J].Food Nutrit Sci,2011,2(2):128-132.
[16] 张晓娟,张燕丽,左冬冬,等.牛蒡子的质量控制、药理作用研究进展[J].中医药信息,2013,30(6):125-128.