艾灸预刺激对阿尔茨海默病大鼠细胞周期蛋白cyclinA、cyclinB的影响
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摘要
目的:通过观察艾灸预刺激对阿尔茨海默病(Alzheimers disease,AD)模型大鼠细胞周期蛋白cyclinA、cyclinB的影响,以探讨艾灸防治AD的作用机理。方法:将SD大鼠随机分为4组,即正常组、假手术组、模型组、预艾灸组,每组各10只,以双侧海马注入Aβ_(1-42)制作AD大鼠造模,造模前预艾灸组采用艾条温和灸"百会""肾俞"穴,采用免疫组化法测定大鼠海马区cyclinA、cyclinB蛋白的表达。结果:经过2个月的艾灸预刺激,大鼠海马区组织中cylinA、cyclinB蛋白的表达较模型组显著降低(P<0.01)。结论:艾灸预刺激可减少AD大鼠过表达的细胞周期相关蛋白,通过减少AD的细胞凋亡来缓解病理改变。
Objective:By investigating the effect of pre-stimulation with moxibustion on cells period adjustment factors-cyclinA and cyclinB in rat models of Alzheimer's disease so as to research its underlying mechanism in preventing and controlling the disease in rats. Methods:All animals were randomly divided into four groups:normal group,sham-operation group,model group,pre-moxibustion group. AD rats were induced by injecting β-Amyloid 1-42(Aβ1-42)into the double hippocampus. Meanwhile,immunohistochemistry was used to determine the expressions of rats' cyclinA and cyclinB in the hippocampus area. Result:Compared with model group, pre-moxibustion group's expressions of cyclinA and cyclin were lowered obviously in the hippocarnpal area. Conclusion:These results imply that pre-stimulation with moxibustion could reverse the expression of cell cycle-related protein in hippocampus area,consequently reduce the pathological changs of Alzheimer's disease.
Objective:By investigating the effect of pre-stimulation with moxibustion on cells period adjustment factors-cyclinA and cyclinB in rat models of Alzheimer's disease so as to research its underlying mechanism in preventing and controlling the disease in rats. Methods:All animals were randomly divided into four groups:normal group,sham-operation group,model group,pre-moxibustion group. AD rats were induced by injecting β-Amyloid 1-42(Aβ1-42)into the double hippocampus. Meanwhile,immunohistochemistry was used to determine the expressions of rats' cyclinA and cyclinB in the hippocampus area. Result:Compared with model group, pre-moxibustion group's expressions of cyclinA and cyclin were lowered obviously in the hippocarnpal area. Conclusion:These results imply that pre-stimulation with moxibustion could reverse the expression of cell cycle-related protein in hippocampus area,consequently reduce the pathological changs of Alzheimer's disease.
引文
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[16] 王占奎, 郭家奎, 赵淑华, 等. “调神通络”针法对脑梗死患者临床疗效及血流变影响的研究 [J]. 针灸临床杂志, 2011, 27(12):1-4.
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[21] Jain RK, Hong DS, Naing A, et al. Novel phase I study combining G1 phase, S phase, and G2/M phase cell cycle inhibitors in patients with advanced malignancies [J]. Cell cycle, 2015, 14 (21):3434-3440.
[22] Lin AB, McNeely SC, Beckmann RP. Achieving Precision Death with Cell-Cycle Inhibitors that Target DNA Replication and Repair [J]. Clinical Cancer Research, 2017, 23(13):3232-3240.
[23] Yamashita T, Nishimura K, Saiki R, et al. Role of polyamines at the G(1)/S boundary and G(2)/M phase of the cell cycle [J]. Int J Biochem Cell B, 2013, 45(6):1042-1050.
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[25] Akan I, Stichelen SOV, Bond MR, et al. Nutrient-driven O-GlcNAc in proteostasis and neurodegeneration [J]. J Neurochem, 2018, 144(1):7-34.
[26] Li DW, Dai CS, Yang XY, et al. GADD45a Regulates Olaquindox-Induced DNA Damage and S-Phase Arrest in Human Hepatoma G2 Cells via JNK/p38 Pathways [J]. Molecules, 2017, 22(1):124.
[27] 贾未玲, 于淼. CyclinB1与肿瘤细胞周期调控 [J]. 哈尔滨商业大学学报(自然科学版), 2016(6):659-662.
[28] Shen X, Liu L, Yin F, et al. Effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells [J]. General and comparative endocrinology, 2012, 177(1):177-186.
[29] Ingham M, Schwartz GK. Cell-Cycle Therapeutics Come of Age [J]. Journal of clinical oncology: official journal of the American Society of Clinical Oncology, 2017, 35(25):2949-2959.
[30] Frade JM, Ovejero-Benito MC. Neuronal cell cycle: the neuron itself and its circumstances [J]. Cell cycle, 2015, 14(5):712-720.
[31] 姚柏春, 袁华, 黄翔. 海马注射Aβ1～40诱导大鼠记忆减退及其脑内细胞周期蛋白A的异常表达 [J]. 郧阳医学院学报, 2004(4):193-196.
[32] Keeney JTR, Swomley AM, Harris JL, et al. Cell Cycle Proteins in Brain in Mild Cognitive Impairment: Insights into Progression to Alzheimer Disease [J]. Neurotox Res, 2012, 22(3):220-230.
[33] Yang Y, Mufson EJ, Herrup K. Neuronal cell death is preceded by cell cycle events at all stages of Alzheimer’s disease [J]. Journal Of Neuroscience, 2003, 23(7):2557-2563.
[34] 罗磊, 孙国杰, 杜艳军. “益肾调督”针灸法对阿尔茨海默病大鼠海马神经元线粒体动力学相关蛋白的影响 [J]. 针刺研究, 2015, 40(4):270-274.
[2] 李华, 蔡超群. 中药提取物治疗阿尔茨海默病的研究进展 [J]. 中国中医药现代远程教育, 2015, 13(19):158-159.
[3] 朱永洙. 老年性痴呆的辨证论治及预防 [J]. 现代医药卫生, 2010(17):2659-2660.
[4] Ballard C, Gauthier S, Corbett A, et al. Alzheimer’s disease [J]. Lancet, 2011, 377(9770):1019-1031.
[5] Yang Y, Varvel NH, Lamb BT, et al. Ectopic cell cycle events link human Alzheimer’s disease and amyloid precursor protein transgenic mouse models [J]. The Journal of neuroscience : the official journal of the Society for Neuroscience, 2006, 26(3):775-784.
[6] 张占鹏, 安红梅, 胡兵, 等. 阿尔茨海默病病理改变与细胞周期相关蛋白关系的研究进展 [J]. 中华实用中西医杂志, 2008, 21 (6):472-475.
[7] Neve RL, McPhie DL. Dysfunction of amyloid precursor protein signaling in neurons leads to DNA synthesis and apoptosis [J]. Biochimica et biophysica acta, 2007, 1772(4):430-437.
[8] Jiao W, Wang Y, Kong L, et al. CART peptide activates the Nrf2/HO-1 antioxidant pathway and protects hippocampal neurons in a rat model of Alzheimer’s disease [J]. Biochemical and biophysical research communications, 2018, 501(4):1016-1022.
[9] Wang H, Jiang TY, Li W, et al. Resveratrol attenuates oxidative damage through activating mitophagy in an in vitro model of Alzheimer’s disease [J]. Toxicol Lett, 2018, 282:100-108.
[10] 司富春, 陈瑞. 痴呆的中医证型和方药规律分析 [J]. 世界中西医结合杂志, 2014, 9(10):1119-1122.
[11] 张玉莲, 张连城, 李强, 等. 660例老年性痴呆患者中医证候学研究 [J]. 中医杂志, 2015, 56(3):235-239.
[12] 刘艺丽, 胡为民. 阿尔兹海默病的治疗进展 [J]. 中国药物与临床, 2014, 14(1):56-60.
[13] 孙璐, 潘隆盛. 阿尔兹海默病外科治疗进展 [J]. 立体定向和功能性神经外科杂志, 2014, 27(6):377-378.
[14] 罗琴琴, 杜艳军. 针灸治疗阿尔茨海默病临床研究进展 [J]. 辽宁中医杂志, 2014, 41(3):594-597.
[15] 周友龙, 韩红艳. 针灸治疗阿尔兹海默病的临床研究进展 [J]. 针灸临床杂志, 2006, 22(11):50-53.
[16] 王占奎, 郭家奎, 赵淑华, 等. “调神通络”针法对脑梗死患者临床疗效及血流变影响的研究 [J]. 针灸临床杂志, 2011, 27(12):1-4.
[17] 李霁, 董竞成, 左传涛, 等. 针刺对脑葡萄糖代谢和功能的影响[J]. 上海针灸杂志, 2001, 20(1):3-5.
[18] 丁红, 王磊. 针灸对缺氧大鼠神经保护作用机制的研究 [J]. 中华保健医学杂志, 2013, 15(3):240-241,248.
[19] Van Leeuwen LA, Hoozemans JJ. Physiological and pathophysiological functions of cell cycle proteins in post-mitotic neurons: implications for Alzheimer’s disease [J]. Acta neuropathologica, 2015, 129(4):511-525.
[20] Kozlov S, Afonin A, Evsyukov I, et al. Alzheimer’s disease: as it was in the beginning [J]. Rev Neuroscience, 2017, 28(8):825-843.
[21] Jain RK, Hong DS, Naing A, et al. Novel phase I study combining G1 phase, S phase, and G2/M phase cell cycle inhibitors in patients with advanced malignancies [J]. Cell cycle, 2015, 14 (21):3434-3440.
[22] Lin AB, McNeely SC, Beckmann RP. Achieving Precision Death with Cell-Cycle Inhibitors that Target DNA Replication and Repair [J]. Clinical Cancer Research, 2017, 23(13):3232-3240.
[23] Yamashita T, Nishimura K, Saiki R, et al. Role of polyamines at the G(1)/S boundary and G(2)/M phase of the cell cycle [J]. Int J Biochem Cell B, 2013, 45(6):1042-1050.
[24] 谢朝阳, 祝其锋. 细胞周期调控与阿尔茨海默病的研究进展 [J]. 中国老年学杂志, 2004, 24(4):379-381.
[25] Akan I, Stichelen SOV, Bond MR, et al. Nutrient-driven O-GlcNAc in proteostasis and neurodegeneration [J]. J Neurochem, 2018, 144(1):7-34.
[26] Li DW, Dai CS, Yang XY, et al. GADD45a Regulates Olaquindox-Induced DNA Damage and S-Phase Arrest in Human Hepatoma G2 Cells via JNK/p38 Pathways [J]. Molecules, 2017, 22(1):124.
[27] 贾未玲, 于淼. CyclinB1与肿瘤细胞周期调控 [J]. 哈尔滨商业大学学报(自然科学版), 2016(6):659-662.
[28] Shen X, Liu L, Yin F, et al. Effect of dehydroepiandrosterone on cell growth and mitochondrial function in TM-3 cells [J]. General and comparative endocrinology, 2012, 177(1):177-186.
[29] Ingham M, Schwartz GK. Cell-Cycle Therapeutics Come of Age [J]. Journal of clinical oncology: official journal of the American Society of Clinical Oncology, 2017, 35(25):2949-2959.
[30] Frade JM, Ovejero-Benito MC. Neuronal cell cycle: the neuron itself and its circumstances [J]. Cell cycle, 2015, 14(5):712-720.
[31] 姚柏春, 袁华, 黄翔. 海马注射Aβ1～40诱导大鼠记忆减退及其脑内细胞周期蛋白A的异常表达 [J]. 郧阳医学院学报, 2004(4):193-196.
[32] Keeney JTR, Swomley AM, Harris JL, et al. Cell Cycle Proteins in Brain in Mild Cognitive Impairment: Insights into Progression to Alzheimer Disease [J]. Neurotox Res, 2012, 22(3):220-230.
[33] Yang Y, Mufson EJ, Herrup K. Neuronal cell death is preceded by cell cycle events at all stages of Alzheimer’s disease [J]. Journal Of Neuroscience, 2003, 23(7):2557-2563.
[34] 罗磊, 孙国杰, 杜艳军. “益肾调督”针灸法对阿尔茨海默病大鼠海马神经元线粒体动力学相关蛋白的影响 [J]. 针刺研究, 2015, 40(4):270-274.