外源性硼酸在大豆制品——腐竹中的形态研究
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摘要
研究外源性硼酸在大豆制品腐竹中的形态分布并鉴定。制备分别添加0%,5%,10%,15%,20%,30%硼酸的腐竹,采用Sephadex G50凝胶柱将腐竹中不同形态的硼化合物进行分离,利用~(11)B NMR扫描鉴定外源性硼酸在腐竹中的形态分布。结果表明:外源性硼主要以游离态硼酸和复合态硼化合物存在于腐竹中,其中约84.7%～93.5%以游离硼酸的形式存在,约6.5%～15.3%以硼复合物的形式存在。
The forms and characterized of additional boric acid in soybean yuba were investigated in the study. Boric acid in different contents(0%, 5%, 10%, 15%, 20%, 30%) were added in yuba, and different forms of boron compounds in yuba were isolated by Sephadex G50 gel column.11 B NMR were utilized in order to identify and quantify the different forms of boron. The results show that additional boron exists in yuba with free boric acid and complex boron compound, of which about 84.7%-93.5% exists in the form of free boric acid, and about 6.5%-15.3%exists in the form of boron complex.
The forms and characterized of additional boric acid in soybean yuba were investigated in the study. Boric acid in different contents(0%, 5%, 10%, 15%, 20%, 30%) were added in yuba, and different forms of boron compounds in yuba were isolated by Sephadex G50 gel column.11 B NMR were utilized in order to identify and quantify the different forms of boron. The results show that additional boron exists in yuba with free boric acid and complex boron compound, of which about 84.7%-93.5% exists in the form of free boric acid, and about 6.5%-15.3%exists in the form of boron complex.
引文
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[19]谢丽燕,林莹,谭瑶瑶,等.正交试验优化传统腐竹制作工艺[J].食品科学,2014,35(2):36-40.
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[22]王璞,林红,朱滨,等.凝胶过滤层析实验中Sephadex的溶胀与回收保存[J].实验技术与管理,2006,23(2):24-25.
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[24]CHUDA Y,OHNISHI-KAMEYAMA M,NAGATAT.Identification of the forms of boron in seaweed by11BNMR[J].Phytochemistry,1997,46(2):209-213.
[2]汪东风.高级食品化学[M].北京:化学工业出版社,2009:163.
[3]GOLDBACH H E,WIMMER M A.Boron in plants and animals:Is there a role beyond cell-wall structure?[J].Journal of Plant Nutrition&Soil Science,2010,170(1):39-48.
[4]陈莉莉,陈江,章荣华.食品中硼本底值调查研究进展[J].浙江预防医学,2016,28(4):371-375.
[5]钟炳南.硼在生命科学中的作用及对人体健康的影响[J].世界元素医学,2005,12(1):49-50.
[6]CULVER B D,HUBBARD S A.Inorganic boron health effects in humans:An aid to risk assessment and clinical judgement[J].J Trace Elem Exp Med,1996,9(4):175-184.
[7]万洋灵,郭顺堂.食品中硼的健康风险与控制研究进展[J].食品科学,2016,37(21):265-270.
[8]李金林,刘林勇,黄丽.大豆及大豆制品中硼砂(硼酸)本底调查[J].江西食品工业,2012(2):44-45.
[9]BOLANOS L,LUKASZEWSKI K,BONILLA I,et al.Why boron?[J].Plant Physiology&Biochemistry,2004,42(11):907-912.
[10]ZITTLE C A.Reaction of borate with substances of biological interest[J].Adv Enzymol Relat Subj Biochem,1951,12:493-527.
[11]黄丽,李金林,郭雪梅,等.大豆中硼形态研究及豆制品中硼形态调查[J].广东化工,2016,43(19):51-52.
[12]曹艇,刘梦溪.食品中非法添加硼砂的危害[J].中国预防医学杂志,2003,4(3):237-238.
[13]FARFAN-GARCIA E D,CASTILLO-MENDIETA NT,CIPROS-FLORES F J,et al.Current data regarding the structure-toxicity relationship of boroncontaining compounds[J].Toxicology Letters,2016,258:115-125.
[14]SCOREI I R.Calcium fructoborate:plant-based dietary boron as potential medicine for cancer therapy[J].Frontiers in Bioscience,2011,3(3):205.
[15]DEMBITSKY V M,AL QUNTAR A A,SREBNIKM.Natural and synthetic small boron-containing molecules as potential inhibitors of bacterial and fungal quorum sensing[J].Chemical Reviews,2011,111(1):209-237.
[16]MOGOSANU G D,BITA,BEJENARU L,et al Calcium fructoborate for bone and cardiovascular health[J].Biol Trace Elem Res,2016,172(2):277-281.
[17]FAWCETT A,PRADEILLES J,WANG Y,et al Photoinduced decarboxylative borylation of carboxylic acids[J].Science,2017,357(6348):283-286.
[18]杨忠明.腐竹制作工艺[J].农业科技通讯,1995(7):29.
[19]谢丽燕,林莹,谭瑶瑶,等.正交试验优化传统腐竹制作工艺[J].食品科学,2014,35(2):36-40.
[20]山瑛.索氏抽提法检测脂肪应注意的问题[J].中国卫生检验杂志,2007,17(6):1138-1138.
[21]LOHSE G.Microanalytical azomethine-H method for boron determination in plant tissue[J].1982,13(2):127-134.
[22]王璞,林红,朱滨,等.凝胶过滤层析实验中Sephadex的溶胀与回收保存[J].实验技术与管理,2006,23(2):24-25.
[23]PFEFFER H,DANNEL F,ROMHELD V.Isolation of soluble boron complexes and their determination together with free boric acid in higher plants[J].Journal of Plant Physiology,1999,154(3):283-288.
[24]CHUDA Y,OHNISHI-KAMEYAMA M,NAGATAT.Identification of the forms of boron in seaweed by11BNMR[J].Phytochemistry,1997,46(2):209-213.