急性镉胁迫对大弹涂鱼血清铁代谢指标的影响
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摘要
研究了不同浓度(0.05、0.25、0.5和5 mg/L)的急性镉胁迫(0~24 h)对大弹涂鱼血清铁含量、总铁结合力、铁蛋白含量等铁代谢指标的影响。结果显示:镉胁迫后大弹涂鱼血清铁浓度均呈先降低后升高的变化(P<0.05),并且镉浓度越高血清铁的变化幅度越大,4个浓度组血清铁的最低值均出现在胁迫后6 h时,24 h时均显著高于胁迫前(P<0.05)。总铁结合力在镉胁迫后呈先降后升的变化,但低浓度下变化差异不显著(P>0.05)。除最高浓度组外,其他浓度组24 h时的总铁结合力均已回升到或超过胁迫前的水平。浓度较低的2个镉胁迫组中血清铁蛋白浓度均未出现显著变化,而另2个浓度较高的组则在胁迫12 h后显著升高(P<0.05),并且升高幅度与镉浓度和胁迫时间均呈正相关,研究结果可为深入研究镉通过干扰铁代谢对大弹涂鱼产生毒性的机理提供参考资料。
The effects of acute cadmium stress on iron metabolism were investigated in Boleophthalmus pectinirostris. Fish was exposed to different concentration Cd~(2+) ( 0. 05,0. 25,0. 5 and 5 mg/L) for 24 h,and the concentration of serum iron,total iron-binding capacity and serum ferritin were detected. The results showed that the concentration of serum iron increased at first and then descended( P< 0. 05),and there had a positive correlation between the variation range of serum iron and Cd~(2+) concentration. In the four experimental groups,the minimal value of serum iron was all appeared at 6 h after Cd~(2+) exposed,and the value at 24 h was all significantly higher than 0 h( P < 0. 05). The total iron binding capacity showed changes of decrease to increase,while the change extent had no significant( P > 0. 05) in 0. 05 mg/L group. At 24 h,total iron binding capacities recovered to or exceed the normal levels( 0 h) except 5 mg/L group. In 0. 05 and 0. 25 mg/L groups,the concentration of serum ferritin had no significant changes during experimental period. In 0. 5 and 5 mg/L groups,the concentration of serum ferritin increased significantly after 12 h( P < 0. 05),and the increased extent was positive correlation to Cd~(2+) concentration. The results could provide reference for investigating the toxicity metabolism of cadmium to B. pectinirostris through interfering iron metabolism.
The effects of acute cadmium stress on iron metabolism were investigated in Boleophthalmus pectinirostris. Fish was exposed to different concentration Cd~(2+) ( 0. 05,0. 25,0. 5 and 5 mg/L) for 24 h,and the concentration of serum iron,total iron-binding capacity and serum ferritin were detected. The results showed that the concentration of serum iron increased at first and then descended( P< 0. 05),and there had a positive correlation between the variation range of serum iron and Cd~(2+) concentration. In the four experimental groups,the minimal value of serum iron was all appeared at 6 h after Cd~(2+) exposed,and the value at 24 h was all significantly higher than 0 h( P < 0. 05). The total iron binding capacity showed changes of decrease to increase,while the change extent had no significant( P > 0. 05) in 0. 05 mg/L group. At 24 h,total iron binding capacities recovered to or exceed the normal levels( 0 h) except 5 mg/L group. In 0. 05 and 0. 25 mg/L groups,the concentration of serum ferritin had no significant changes during experimental period. In 0. 5 and 5 mg/L groups,the concentration of serum ferritin increased significantly after 12 h( P < 0. 05),and the increased extent was positive correlation to Cd~(2+) concentration. The results could provide reference for investigating the toxicity metabolism of cadmium to B. pectinirostris through interfering iron metabolism.
引文
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[21]金慧英,胡惠民,周雍,等.急性镉中毒的肝脏损伤机制及金属硫蛋白的保护作用[J].中华劳动卫生职业病杂志,1998,16(1):43-46.
[22]候韬,徐燕平.缺铁性贫血患者检测血清铁和转铁蛋白的临床意义[J].中国现代医生,2010,48(29):155-156.
[23]陈静,赵永亮,王丹.铁蛋白研究现状[J].河南师范大学学报(自然科学版),2010,38(1):152-155.
[24]PEAD S,DURRANT E,WEBB B,et al.Metal ion binding to aop,holo,and reconstituted horse spleen ferritin[J].Inorg Biochem,1995,59(1):15-27.
[25]LI CH,LI Z,LI Y,et al.A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity[J].Plo S One,2012,7(12):e51428.
[26]初双双,张云云,苏倡,等.可口革囊星虫(Phascolosoma esculenta)重组铁蛋白富集Pb2+等重金属特性和功能研究[J].海洋与湖沼,2015,46(6):1358-1364.
[27]刘俊峰,谢涛,鞠萍,等.铁蛋白铁含量在肝脏疾病诊断中的临床意义[J].实用医技杂志,2005,12(9):2340-2342.
[28]赵花,张红.肝炎患者血清铁蛋白测定的临床意义[J].中国误诊学杂志,2008,8(28):6870.
[29]KLAUSNER R D,ROUAULT T A,HARFORD J B.Regulating the fate of mRNA:the control of cellular iron metabolism[J].Cell,1993,72(1):19-28.
[2]蔡福龙,陈英,许丕安,等.大弹涂鱼(Boleophthalmus pectinirostris)作为放射性核素指示物的研究[J].环境科学学报,1992,12(3):282-287.
[3]FENG T,GUO X Q,ZHENG W Y,et al.Detection of DNA strand breaks in the liver of Boleophthalmus pectinirostris treated with benzo(a)pyrene[J].Bulletin of Environmental Contamination and Toxicology,2003,71(2):263-269.
[4]上官旖旎,赵金龙,陈婷,等.对二甲苯对大弹涂鱼肝脏和白肌抗氧化酶活性的影响[J].水产科学,2011,30(1):6-10.
[5]ANSARI T M,MARR I L,TARIQ N.Heavy metals in marine pollution perspective-a mini review[J].Journal of Applied Sciences,2004,4(1):1-20.
[6]LUPARELLO C,LONGO A,VETRANO M.Exposure to cadmium chloride influences astrocyte-elevated gene-1(AEG-1)expression in MDA-MB231 human breast cancer cells[J].Biochimie,2012,94(1):207-213.
[7]叶帅东,李明云,章志盈.镉离子对大弹涂鱼急性攻毒试验[J].宁波大学学报(理工版),2007,20(3):320-323.
[8]黄福勇,李明云,竺俊全,等.急性镉中毒对大弹涂鱼血细胞同工酶表达的影响[J].上海水产大学学报,2004,13(4):289-292.
[9]金春华,李明云,刘伟成,等.镉胁迫对大弹涂鱼(Boleophthalmus pectinirostris)血细胞遗传损伤的研究[J].海洋与湖沼,2010,41(1):80-83.
[10]张玉平,孙振中,郝永梅.镉对大弹涂鱼肝脏超氧化物歧化酶活性的影响[J].水产科学,2008,27(2):79-81.
[11]FILIPICˇM.Mechanisms of cadmium induced genomic instability[J].Mutation Research/Fundamental and Molecular Mechanisms of Mutagenesis,2012,733(1-2):69-77.
[12]JIN Y H,CLARK A B,SLEBOS R J,et al.Cadmium is a mutagen that acts by inhibiting mismatch repair[J].Nature Genetics,2003,34(3):326-329.
[13]MOULIS J M.Cellular mechanisms of cadmium toxicity related to the homeostasis of essential metals[J].Biometals,2010,23(5):877-896.
[14]KIM D W,KIM K Y,CHOI B S,et al.Regulation of metal transporters by dietary iron,and the relationship between body iron levels and cadmium uptake[J].Arch Toxicol,2007,81(5):327-334.
[15]陈洁,熊邦喜,顾钱洪,等.内源及外援因子对鱼类血液生理生化指标的影响[J].湖北农业科学,2011,50(9):1861-1865.
[16]弭希峰,黄子成,王木成,等.非酒精性脂肪肝患者血清铁、转铁蛋白、血清铁蛋白水平测定[J].上海医药,2006,27(11):517-518.
[17]WLOSTOWSKI T,KRASOWSKA A,BONDA E.An iron-rich diet protects the liver and kindeys against cadmium-induced injury in the bank wole(Clethrionomys glareolus)[J].Ecotoxicol Environ Sar,2003,54(2):194-198.
[18]黄林.镉对南方鲇血液学指标和造血器官的影响[D].重庆:西南大学,2010.
[19]刘宗平,马卓,李文范,等.镉对绵羊的毒理学研究[J].畜牧兽医学报,1996,27(6):546-553.
[20]马振祥,姜声扬,张卫兵.实验性镉中毒致小鼠血液学指标改变及硫酸锌的保护作用[J].中国职业医学,2004,31(5):36-38.
[21]金慧英,胡惠民,周雍,等.急性镉中毒的肝脏损伤机制及金属硫蛋白的保护作用[J].中华劳动卫生职业病杂志,1998,16(1):43-46.
[22]候韬,徐燕平.缺铁性贫血患者检测血清铁和转铁蛋白的临床意义[J].中国现代医生,2010,48(29):155-156.
[23]陈静,赵永亮,王丹.铁蛋白研究现状[J].河南师范大学学报(自然科学版),2010,38(1):152-155.
[24]PEAD S,DURRANT E,WEBB B,et al.Metal ion binding to aop,holo,and reconstituted horse spleen ferritin[J].Inorg Biochem,1995,59(1):15-27.
[25]LI CH,LI Z,LI Y,et al.A ferritin from Dendrorhynchus zhejiangensis with heavy metals detoxification activity[J].Plo S One,2012,7(12):e51428.
[26]初双双,张云云,苏倡,等.可口革囊星虫(Phascolosoma esculenta)重组铁蛋白富集Pb2+等重金属特性和功能研究[J].海洋与湖沼,2015,46(6):1358-1364.
[27]刘俊峰,谢涛,鞠萍,等.铁蛋白铁含量在肝脏疾病诊断中的临床意义[J].实用医技杂志,2005,12(9):2340-2342.
[28]赵花,张红.肝炎患者血清铁蛋白测定的临床意义[J].中国误诊学杂志,2008,8(28):6870.
[29]KLAUSNER R D,ROUAULT T A,HARFORD J B.Regulating the fate of mRNA:the control of cellular iron metabolism[J].Cell,1993,72(1):19-28.