水体动荡胁迫对大黄鱼血清生化指标的影响
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摘要
对大黄鱼进行模拟台风所致水体动荡胁迫,检测胁迫前(0 h)及胁迫后0.5、1、2、4、8、24 h时血清生化指标的变化.结果表明:大黄鱼血清总蛋白(TP)和总胆固醇(CHOL)在胁迫后0.5~24 h期间浓度均持续降低;谷丙转氨酶(ALT)活性在胁迫后0.5~1 h期间显著升高,之后显著降低,浓度仅为胁迫前的一半,并在2~24 h期间始终处于这一低水平;谷草转氨酶(AST)活性在胁迫后持续显著升高,胁迫后24 h时的活性为胁迫前的6.5倍;肌酐(CREA)在胁迫后0.5 h时即显著升高并达到最大值,1~8 h期间基本降至正常水平,但在24 h时显著降低,仅为胁迫前的1/4;乳酸脱氢酶(LDH)活性在胁迫后2~24 h期间均显著高于正常水平;碱性磷酸酶(ALP)活性在胁迫后2 h时显著升高,并在2~24 h期间都处于较高水平;血清Na~+、C1~-、K~+和Ca~(2+)浓度在胁迫后均出现了显著性升高.血清生化指标的变化提示水体动荡胁迫引起的应激反应可能导致大黄鱼肝脏、心脏受损以及免疫降低.
In this study,stress was introduced to large yellow croaker(Larimichthys crocea) using a typhoon-induced water unrest simulator.The serum biochemical parameters were detected at 0.5,1,2,4,8 and 24 h following stress,and the untreated samples were used as control(0 h).The results showed that serum total protein(TP) and cholesterol(CHOL) decreased consecutively from 0.5 h to 24 h following stress.The activity of alanine transaminase(ALT) increased significantly during 0.5-1 h and decreased significantly afterwards.The activities during 2-24 h were only half those of the control.The activity of aspartate transaminase(AST) increased significantly from 0.5 h to 24 h,and the activity at 24 h increased 6.5 times compared to that of 0 h.The peak value of creatinine(CREA) activity appeared at 0.5 h.During the period of 1-8 h,the activities were recovered to the level corresponding to the control group;whereas at 24 h the activity decreased significantly to 25% of the control.During 2-24 h,the activities of lactic dehydrogenase(LDH) were significantly higher than that of 0 h.The activity of alkaline phosphatase(ALP) had a significant increase at 2 h,and maintained relatively high level during 2-24 h.And the concentrations of Na~+、C1~-、K~+ and Ca~(2+) in serum all increased significantly after water unrest stress.The changes of serum biochemical parameters of P.crocea indicated that the stress of water unrest could lower immunity as well as impair liver and heart.
In this study,stress was introduced to large yellow croaker(Larimichthys crocea) using a typhoon-induced water unrest simulator.The serum biochemical parameters were detected at 0.5,1,2,4,8 and 24 h following stress,and the untreated samples were used as control(0 h).The results showed that serum total protein(TP) and cholesterol(CHOL) decreased consecutively from 0.5 h to 24 h following stress.The activity of alanine transaminase(ALT) increased significantly during 0.5-1 h and decreased significantly afterwards.The activities during 2-24 h were only half those of the control.The activity of aspartate transaminase(AST) increased significantly from 0.5 h to 24 h,and the activity at 24 h increased 6.5 times compared to that of 0 h.The peak value of creatinine(CREA) activity appeared at 0.5 h.During the period of 1-8 h,the activities were recovered to the level corresponding to the control group;whereas at 24 h the activity decreased significantly to 25% of the control.During 2-24 h,the activities of lactic dehydrogenase(LDH) were significantly higher than that of 0 h.The activity of alkaline phosphatase(ALP) had a significant increase at 2 h,and maintained relatively high level during 2-24 h.And the concentrations of Na~+、C1~-、K~+ and Ca~(2+) in serum all increased significantly after water unrest stress.The changes of serum biochemical parameters of P.crocea indicated that the stress of water unrest could lower immunity as well as impair liver and heart.
引文
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[19]张卫东.鱼用抗应激饲料添加剂的研究与应用[J].河南水产,2007,72(3):45-46.
[20]潘孝毅,张琴,李俊,等.饲料中添加甘氨酸可提高大黄鱼(Larimichthys crocea)的抗氧化和抗应激能力[J].渔业科学进展,2017,38(2):91-98.
[21]涂振波,王曙,李明云,等.添加Vc、甜菜碱饲料养殖大黄鱼的效果试验[J].科学养鱼,2012(9):72-73.
[22]张达云,申屠基康.中草药在水生动物机体调节和抗应激中的应用[J].现代农业科技,2016(10):256-257.
[2]陈昌福.应激与鱼类应激性疾病[J].水利渔业,1998(5):7-9.
[3]Xu J L,Yan X J,Chen D Y,et al.Metabolite change of cage-farming Pseudosciaena crocea during recovery process after typhoon[J].Chinese Journal of Analytical Chemistry,2010,38(9):1249-1255.
[4]李明云,王曙,苗亮,等.一种巨浪模拟器:中国,ZL201020550153.0[P].2011-04-27.
[5]王曙,李明云,苗亮,等.台风引起网箱内水体动荡模拟装置的制作与应用[J].宁波大学学报(理工版),2012,25(2):26-28.
[6]周玉,郭文场,杨振国,等.鱼类血液学指标研究的进展[J].上海水产大学学报,2001,10(2):163-165.
[7]洪磊,张秀梅.环境胁迫对鱼类生理机能的影响[J].海洋科学进展,2004,22(1):114-121.
[8]冀德伟,李明云,王天柱,等.不同低温胁迫时间对大黄鱼血清生化指标的影响[J].水产科学,2009,28(1):1-4.
[9]张晓丽,胡玉珍,李明云,等.养殖大黄鱼在自然海区降温不同阶段抗氧化水平及血清酶活性的变化[J].海洋科学,2013,37(11):27-34.
[10]管标,温海深,刘群,等.急性温度胁迫对虹鳟血细胞和血清生化组分的影响[J].海洋与湖沼,2014,28(3):64-69.
[11]常玉梅,曹鼎臣,孙效文,等.低温胁迫对鲤鱼血清生化指标的影响[J].水产学杂志,2006,19(2):71-75.
[12]张磊,樊启学,赵志刚,等.慢性拥挤胁迫对鲤生长及血液生化指标的影响[J].大连水产学院学报,2008,22(6):465-469.
[13]王文博,汪建国,李爱华,等.拥挤胁迫后鲫鱼血液皮质醇和溶菌酶水平的变化及对病原的敏感性[J].中国水产,2004,1(5):408-412.
[14]徐力文,苏游禄,刘广峰,等.急性盐度胁迫下军曹鱼稚鱼应激反应的血清学指标[J].华南农业大学学报,2007,28(2):91-94.
[15]徐力文,冯娟,郭志勋,等.盐度对军曹鱼稚鱼血液生理生化及鳃Na+,K+-ATPase活性的影响[J].海洋环境科学,2009,27(6):602-606.
[16]王涛,苗亮,李明云,等.突降盐度胁迫对大黄鱼血清生理生化及鳃丝Na+,K+-ATPase酶活性的影响[J].海洋与湖沼,2013,44(2):422-426.
[17]Dabrowska H,Dabrowski K,Meyer-Burgdorff K,et al.The effect of large doses of vitamin C and magnesium on stress responses in common carp,Cyprinus carpio[J].Comparative Biochemistry&Physiology A Comparative Physiology,1991,99(4):681-685.
[18]周传朋,刘波,谢骏,等.抗应激制剂对运输团头鲂血清生化和肝脏抗氧化指标的影响[J].水产科学,2014,33(1):8-14.
[19]张卫东.鱼用抗应激饲料添加剂的研究与应用[J].河南水产,2007,72(3):45-46.
[20]潘孝毅,张琴,李俊,等.饲料中添加甘氨酸可提高大黄鱼(Larimichthys crocea)的抗氧化和抗应激能力[J].渔业科学进展,2017,38(2):91-98.
[21]涂振波,王曙,李明云,等.添加Vc、甜菜碱饲料养殖大黄鱼的效果试验[J].科学养鱼,2012(9):72-73.
[22]张达云,申屠基康.中草药在水生动物机体调节和抗应激中的应用[J].现代农业科技,2016(10):256-257.