杞参膏对干眼症SD大鼠血清Mig、IP-10影响随机平行对照研究
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摘要
[目的]观测杞参膏对干眼症SD大鼠血清Mig、IP-10影响。[方法]使用随机平行对照方法,30只SD大鼠喂养7d,称重后按照随机数字法分为3组,空白对照组、模型对照组、杞参膏组,10只/组。适应喂养7d后,暴露和皮下注射东莨菪碱法复制干眼症大鼠模型,连续28d。灌胃干预,模型对照组:生理盐水,1次/d;杞参膏组:杞参膏汤剂,1次/d;均连续56d。观测角膜病理、血清Mig、IP-10含量。[结果]空白对照组大鼠角膜未见异常。模型对照组大鼠角膜上皮层细胞增多,层数增加,局部扁平细胞偶见不规则的脱失;基质层可见增厚表现,偶见水肿,大量炎性细胞浸润。杞参膏组大鼠角膜各层尚为完整,上皮层光滑度尚可,未见扁平细胞脱失现象。与空白对照组比较,模型对照组大鼠血清Mig、IP-10含量显著升高(P<0.01);与模型对照组比较,杞参膏组大鼠血清Mig、IP-10含量显著降低(P<0.01,P<0.05)。[结论]杞参膏可减轻干眼症模型大鼠角膜病理损伤,降低干眼症模型大鼠血清Mig、IP-10含量,可能是杞参膏抗炎作用的机制之一。
[Objective] To observe the Qi-shen ointment for dry eyes SD rats serum Mig, IP-10. [Methods] Using random parallel control method, 30 SD rats acclimate for 7 d, divided into three groups according to random number method, blank control group, model control group, Qi-shen ointment group, 10 rats/group. After acclimatefor 7d, Adopt the method of exposure and subcutaneous injection of Scopolamine for dry eyes rats, continuous 28 d. The methods of gavage, model control group: normal saline, once a day; Qi-shenointment group: Qi-shen ointment, once a day; continuous 56 d. to Observe the pathological change of cornea, Mig, IP-10 levels in serum. [Results] The pathological change of the blank control group rat cornea didn't show abnormal. model control group rats: the cortical cells increases, the occasional irregular depigmentation local squamous cells; Thickening of corneal stroma visible performance, a large number of inflammatory cells infiltration. the pathological change of Qi-shen ointment group rat cornea is complete, theepithelial layer is smooth, and did not see pinacocytes deletions phenomenon. Compared with blank control group, model control group rats serum Mig, IP-10 content significantly increased(P<0.01); Compared with model control group, Qi-shencream group rats serum Mig, IP-10 levels decreased significantly(P<0.01, P<0.05). [Conclusion] Qi-shen cream can alleviate the pathological change of the model rat cornea, reduce dry eyes model rats serum Mig, IP-10 levels, may be one of Qi-shen ointment paste the mechanism of anti-inflammatory effects.
[Objective] To observe the Qi-shen ointment for dry eyes SD rats serum Mig, IP-10. [Methods] Using random parallel control method, 30 SD rats acclimate for 7 d, divided into three groups according to random number method, blank control group, model control group, Qi-shen ointment group, 10 rats/group. After acclimatefor 7d, Adopt the method of exposure and subcutaneous injection of Scopolamine for dry eyes rats, continuous 28 d. The methods of gavage, model control group: normal saline, once a day; Qi-shenointment group: Qi-shen ointment, once a day; continuous 56 d. to Observe the pathological change of cornea, Mig, IP-10 levels in serum. [Results] The pathological change of the blank control group rat cornea didn't show abnormal. model control group rats: the cortical cells increases, the occasional irregular depigmentation local squamous cells; Thickening of corneal stroma visible performance, a large number of inflammatory cells infiltration. the pathological change of Qi-shen ointment group rat cornea is complete, theepithelial layer is smooth, and did not see pinacocytes deletions phenomenon. Compared with blank control group, model control group rats serum Mig, IP-10 content significantly increased(P<0.01); Compared with model control group, Qi-shencream group rats serum Mig, IP-10 levels decreased significantly(P<0.01, P<0.05). [Conclusion] Qi-shen cream can alleviate the pathological change of the model rat cornea, reduce dry eyes model rats serum Mig, IP-10 levels, may be one of Qi-shen ointment paste the mechanism of anti-inflammatory effects.
引文
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[14]彭清华,姚小磊,彭俊,等.密蒙花提取物对干眼症雄兔泪腺局部炎症反应影响的研究[J].中华中医药学刊,2010(7):1351-1356.
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[2]宿梦苍,郝晓琳,张仲臣.干眼症眼表损害炎症机制[J].国际眼科杂志,2015(5):821-824.
[3]张翠红,张琳琳,张丹,等.电针对实验性干眼症泪腺和眼表组织形态学的影响[J].Journal of Acupuncture and Tuina Science,2016(5):320-327.
[4]陈星如,高卫萍.针刺对东莨菪碱诱导的兔干眼模型的作用及机制探讨[J].中国中医眼科杂志,2016(5):281-284.
[5]杨雯,田琪,马贤德,等.中西医结合疗法对暴露性干眼症大鼠的干预研究[J].湖南中医杂志,2014(3):126-129.
[6]赵晓慧,侯平.大鼠衰老与血浆中T3、T4、TSH含量变化相关性随机平行对照研究[J].实用中医内科杂志,2015,29(7):131-133.
[7]张慧芹,李颖,许永根.实验性干眼大鼠的角结膜上皮组织学改变[J].中国比较医学杂志,2016(12):45-50.
[8]夏羽佳,田德安,曾丹,等.CXCL9对单个核细胞的趋化作用及其机制探讨[J].中国组织化学与细胞化学杂志,2012(4):319-322.
[9]叶鳞泓,叶蕾,唐丽媛,等.槲寄生滴眼液治疗去势雄兔干眼症实验研究[J].眼科新进展,2016(10):915-918;922.
[10]蔡伟浩,彭玲,陆晓和,等.omega-3治疗大鼠干眼的实验研究[J].眼科新进展,2016(9):801-805.
[11]田明霞,王传富.暴露性干眼症大鼠模型角膜上皮细胞凋亡相关基因的表达[J].眼科新进展,2006(10):747-750.
[12]马晓昀,殷莲华.炎症与干眼症的研究进展[J].复旦学报(医学版),2009(4):495-498.
[13]侯乐,谢立科,张明明.炎症和抗炎治疗与干眼的关系[J].中国中医眼科杂志,2010(5):307-309.
[14]彭清华,姚小磊,彭俊,等.密蒙花提取物对干眼症雄兔泪腺局部炎症反应影响的研究[J].中华中医药学刊,2010(7):1351-1356.
[15]董西园.医级临证综合[M].北京:中国中医药出版社,2015.
[16]钱乙.小儿药证直诀[M].天津:天津科学技术出版社,2005.
[17]高晓山.中药药性论[M].北京:人民卫生出版社,1992.