绞股蓝水提物对束缚水浸应激性胃溃疡模型大鼠的预防作用
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摘要
目的:考察绞股蓝水提物对应激性胃溃疡模型大鼠的预防作用。方法:将60只大鼠随机分为正常组、模型组、阳性组(三九胃泰颗粒,3.69 g/kg)和绞股蓝水提物高、中、低剂量组(4.0、2.0、1.0 g/kg,以生药计),每组10只,ig给药,每天1次,连续1周。给药结束2 h后,除正常组外,其余各组大鼠均采用束缚水浸法复制应激性胃溃疡模型。造模3 h后,测定溃疡指数(UI)、溃疡抑制率,测定血清中超氧化物歧化酶(SOD)、丙二醛(MDA)以及胃黏膜组织中肿瘤坏死因子α(TNF-α)、前列腺素E2(PGE2)、一氧化氮(NO)、内皮素1(ET-1)水平。结果:与正常组比较,模型组大鼠血清中SOD水平降低、MDA水平升高(P<0.05),胃黏膜组织中PGE2、NO水平降低,TNF-α、ET-1水平升高(P<0.05);与模型组比较,各给药组大鼠UI明显降低、上述指标均明显改善,且具有一定的剂量依赖性,其中绞股蓝水提物高剂量组大鼠胃黏膜组织中PGE2、ET-1改善较阳性组更明显,差异均有统计学意义(P<0.05)。结论:绞股蓝水提物可通过调控血清SOD-MDA平衡和胃黏膜组织NO-ET-1平衡、减轻胃黏膜损伤程度等方式发挥其预防胃溃疡的作用。
OBJECTIVE:To investigate the preventive effect of Gynostemma pentaphyllum water extract on model rats with stress-induced gastric ulcer. METHODS:60 rats were randomly divided into normal group,model group,positive group(Sanjiu weitai granules,3.69 g/kg)and G. pentaphyllum water extract high-dose,medium-dose,low-dose groups(4.0,2.0,1.0 g/kg,calculated by crude drug),10 in each group. All rats were intragastrically administrated,once a day,for one week. After 2 h of administration,except for normal group,rats were induced to stress gastric ulcer model by water immersion method in other groups.After 3 h of modeling,ulcer index(UI),ulcer inhibition rate were detected;superoxide dismutase(SOD)and malondialdehyde(MDA)in serum,tumor necrosis factor-α(TNF-α),prostaglandin E2(PGE2),nitric oxide(NO)and endothelin-1(ET-1)levels in gastric mucosal tissue were determined. RESULTS:Compared with normal group,SOD level in model group was decreased,while MDA level was increased in serum(P<0.05);PGE2,NO levels were decreased,TNF-α,ET-1 levels were increased in gastric mucosal tissue(P<0.05). Compared with model group,UI in administration groups were obviously decreased,above-mentioned indexes were obviously improved,which showed certain dose-dependence. And the levels of PEG2,ET-1 of gastric mucosaltissue in G. pentaphyllum water extract high-dose group improved more obviously than positive group,with statistical significances(P<0.05). CONCLUSIONS:G. pentaphyllum water extract plays a role in preventing gastric ulcer by regulating serum SOD-MDA balance,gastric mucosal tissue NO-ET-1 balance and reduce the degree of gastric mucosa injury,etc.
OBJECTIVE:To investigate the preventive effect of Gynostemma pentaphyllum water extract on model rats with stress-induced gastric ulcer. METHODS:60 rats were randomly divided into normal group,model group,positive group(Sanjiu weitai granules,3.69 g/kg)and G. pentaphyllum water extract high-dose,medium-dose,low-dose groups(4.0,2.0,1.0 g/kg,calculated by crude drug),10 in each group. All rats were intragastrically administrated,once a day,for one week. After 2 h of administration,except for normal group,rats were induced to stress gastric ulcer model by water immersion method in other groups.After 3 h of modeling,ulcer index(UI),ulcer inhibition rate were detected;superoxide dismutase(SOD)and malondialdehyde(MDA)in serum,tumor necrosis factor-α(TNF-α),prostaglandin E2(PGE2),nitric oxide(NO)and endothelin-1(ET-1)levels in gastric mucosal tissue were determined. RESULTS:Compared with normal group,SOD level in model group was decreased,while MDA level was increased in serum(P<0.05);PGE2,NO levels were decreased,TNF-α,ET-1 levels were increased in gastric mucosal tissue(P<0.05). Compared with model group,UI in administration groups were obviously decreased,above-mentioned indexes were obviously improved,which showed certain dose-dependence. And the levels of PEG2,ET-1 of gastric mucosaltissue in G. pentaphyllum water extract high-dose group improved more obviously than positive group,with statistical significances(P<0.05). CONCLUSIONS:G. pentaphyllum water extract plays a role in preventing gastric ulcer by regulating serum SOD-MDA balance,gastric mucosal tissue NO-ET-1 balance and reduce the degree of gastric mucosa injury,etc.
引文
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[11]Ye B,Zhou PY,Jia M,et al,Absence of NF-kappa B subunit p50 ameliorates cold immobilization stress-induced gastric ulcers[J].Bilchem Biophys Res Commun,2013,434(3):547-551.
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[13]Jo IG,Park D,Yung KJ,et al.Inhibitory effects of aβ-dunnione compound MB12662 on gastric secretion and ulcers[J].Lab Anim Res,2013,29(3):178-181.
[14]孙菁,房静远,盛剑秋,等.三九胃泰颗粒与奥美拉唑联用治疗功能性消化不良疗效和安全性的多中心、随机、双盲、双模拟、平行对照临床试验[J].中华消化杂志,2013,33(4):269-273.
[2]王海岭,黄洪勇,王传升,等.总丹参酮对模型大鼠疮疡的促愈合作用研究[J].中国药房,2015,26(19):2635-2637.
[3]李志坤,王福文.药物治疗应激性胃溃疡的研究进展[J].医学研究生学报,2015,28(1):110-112.
[4]黄志远.黄芪多糖对大鼠实验性胃溃疡的影响[J].中国医药导报,2015,12(15):16-19.
[5]王宏涛,叶云,肖顺林,等.绞股蓝乙酸乙酯提取物对胃溃疡大鼠胃黏膜血管活性物质的影响[J].中国中医急症,2008,17(10):1437-1438.
[6]张玉玉,祝文兴,孙一耕,等.大鼠束缚-浸水应激模型体温降低与应激性胃溃疡的关系[J].生物医学工程研究,2007,26(3):282-285.
[7]林科名,丁世兰,王强松,等.左金丸总生物碱对束缚水浸应激性胃溃疡模型大鼠神经体液调节的影响[J].中国药理学通报,2013,29(3):401-405.
[8]宋晓宁,曾万玲,靳凤云,等.戊己丸3种剂型的镇痛、止泻及抗急性胃溃疡作用比较[J].中国实验方剂学杂志,2014,20(21):158-161.
[9]高洋.不同针灸配穴防治应激性胃溃疡大鼠的差异蛋白表达研究[D].长春:长春中医药大学,2009.
[10]史李娜,王雪飞,马桂芝,等.石榴皮多酚有效部位单次给药毒性及对无水乙醇致大鼠胃溃疡的保护作用[J].中国药理学通报,2015,31(5):709-715.
[11]Ye B,Zhou PY,Jia M,et al,Absence of NF-kappa B subunit p50 ameliorates cold immobilization stress-induced gastric ulcers[J].Bilchem Biophys Res Commun,2013,434(3):547-551.
[12]Yan B,Shi J,Xiu LJ,et al.Xiaotan tongfu granules contribute to the prevention of stress ulcers[J].World J Gastroenterol,2013,19(33):5473-5484.
[13]Jo IG,Park D,Yung KJ,et al.Inhibitory effects of aβ-dunnione compound MB12662 on gastric secretion and ulcers[J].Lab Anim Res,2013,29(3):178-181.
[14]孙菁,房静远,盛剑秋,等.三九胃泰颗粒与奥美拉唑联用治疗功能性消化不良疗效和安全性的多中心、随机、双盲、双模拟、平行对照临床试验[J].中华消化杂志,2013,33(4):269-273.