辣椒素对浸水-束缚应激大鼠胃动力作用及机制研究
|
摘要
目的:研究辣椒素(CAP)对浸水束缚应激(模型)导致的大鼠胃动力障碍的作用及其机制。方法:选取雄性SPF级SD大鼠40只随机均分为对照组、模型组、模型组+CAP组和CAP组4组,每组10只。采用浸水束缚应激法建立胃动力障碍大鼠模型,胃管注入CAP 1 mg/g,连续4周。实验末测定大鼠胃酚红排空率,并取胃窦组织用RT-PCR方法检测c-kit m RNA、SCF m RNA的转录水平。结果:1胃酚红排空率:CAP组胃酚红排空率显著高于对照组和模型组(P<0.05),模型组+CAP组、对照组胃酚红排空率显著高于模型组(P<0.05);2c-kit m RNA、SCF m RNA的转录水平:模型组c-kit m RNA转录水平显著低于对照组,CAP组c-kit m RNA转录水平显著高于模型组(P<0.05);模型+CAP组、CAP组SCF m RNA转录水平显著高于对照组和模型组(P<0.05),对照组SCF m RNA转录水平显著高于模型组(P<0.05)。结论:小剂量CAP 4周可促进正常大鼠及模型大鼠胃动力,其机制可能与CAP调节SCF的表达有关,但与c-kit、Cajal间质细胞的相关性尚不明确。
Objective: To investigate the effects and mechanisms of capsaicin(CAP) on gastric motility in water immersion restraint stress rats. Methods: Forty male SD rats were randomly divided into 4 groups(n=10):normal control group, model group, model +CAP group, and CAP group. The model of water immersion restraint stress was created by injecting capsaicin 1mg/g for 4 consecutive weeks. At the end of the experiment, gastric emptying rate of phenol red was tested and the expression of c-kit and SCF m RNAs were evaluated in the gastric antrum tissues. Results: 1 The gastric emptying rate in CAP group was significantly higher than that in the control and model groups( P < 0.05), and the gastric emptying rate in the model+CAP and control groups were significantly higher than that in the model group( P < 0.05). 2 The expression of c-kit m RNA in model group was significantly lower than that in the control group(P < 0.05), and the expression of c-kit m RNA in CAP group was significantly higher than that in the model group( P < 0.05). The expression of SCF m RNA in model+CAP and CAP groups was significantly higher than that in the control and model groups(P < 0.05), and the expression of SCF m RNA in control group was significantly higher than that in the model group( P < 0.05). Conclusion:The CAP fed for 4 weeks could improve the gastric emptying rate in the normal and WIRS rats. The effect of CAP on gastric motility may come from the altered expression of SCF, but have no obvious relationship with interstitial cells of Cajal and the expression of c-kit.
Objective: To investigate the effects and mechanisms of capsaicin(CAP) on gastric motility in water immersion restraint stress rats. Methods: Forty male SD rats were randomly divided into 4 groups(n=10):normal control group, model group, model +CAP group, and CAP group. The model of water immersion restraint stress was created by injecting capsaicin 1mg/g for 4 consecutive weeks. At the end of the experiment, gastric emptying rate of phenol red was tested and the expression of c-kit and SCF m RNAs were evaluated in the gastric antrum tissues. Results: 1 The gastric emptying rate in CAP group was significantly higher than that in the control and model groups( P < 0.05), and the gastric emptying rate in the model+CAP and control groups were significantly higher than that in the model group( P < 0.05). 2 The expression of c-kit m RNA in model group was significantly lower than that in the control group(P < 0.05), and the expression of c-kit m RNA in CAP group was significantly higher than that in the model group( P < 0.05). The expression of SCF m RNA in model+CAP and CAP groups was significantly higher than that in the control and model groups(P < 0.05), and the expression of SCF m RNA in control group was significantly higher than that in the model group( P < 0.05). Conclusion:The CAP fed for 4 weeks could improve the gastric emptying rate in the normal and WIRS rats. The effect of CAP on gastric motility may come from the altered expression of SCF, but have no obvious relationship with interstitial cells of Cajal and the expression of c-kit.
引文
1.Mozsik G.Capsaicin as new orally applicable gastroprotective and therapeutic drug alone or in combination with nonsteroidal anti-inflammatory drugs in healthy human[J].Prog Drog Res,2014,68:209-258.
2 .Debreceni A,Abdel-Salam OM,Figler M,et al.Capsaicin increases gastric emptying rate in healthy human subjects measured by 13C-labeled octanoic acid breath test[J].J Physiol Paris,1999,93(5):455-460.
3 .Matsumoto K,Kurosawa E,Terui H,et al.Localization of TRPV1 and contractile effect of capsaicin in mouse large intestine:high abundance and sensitivity in rectum and distal colon[J].Am J Physiol Gastrointest Liver Physiol,2009,297(2):G348-360.
4 .Bartho L,Lenard L,Patacchini R,et al.Tachykinin receptors are involved in the"local efferent"motor response to capsaicin in the guinea-pig small intestine and oesophagus[J].Neuroscience,1999,90(1):221-228.
5 .Bartho L,Benko R,Patacchini R,et al.Effects of capsaicin on visceral smooth muscle:a valuable tool for sensory neurotransmitter identification[J].Eur J Pharmacol,2004,500(1-3):143-157.
6 .李毅,齐清会.胃肠道神经-Cajal间质细胞-平滑肌网络研究进展[J].国际消化病杂志2007,27(3):225-227.
7 .Lammers WJ,Stephen B.Origin and propagation of individual slow waves along the intact feline small intestine[J].Exp Physiol,2008,93(3):334-346.
8 .Apoznanski W,Koleda P,Wozniak Z,et al.The distribution of interstitial cells of Cajal in congenital ureteropelvic junction obstruction[J].Int Urol Nephrol,2013,45(3):6077-612.
9 .张虹玺.养荣润肠舒合剂对慢传输型便秘模型大鼠结肠中c-Kit、SP与VIP的影响[J].中国药房,2015,26(25):3476-3478.
10 .袁成代,高庆剑.温阳健脾法治疗便秘50例[J].河南中医,2015,35(7):1619-1620.
11 .Huizinga JD,Zarate N,Farrugia G.Physiology,injury,and recovery of interstitial cells of Cajal:basic and clinical science[J].Gastroenterology,2009,137(5):1548-1556.
12 .Sanders KM,Koh SD,Ward SM.Interstitial cells of cajal as pacemakers in the gastrointestinal tract[J].Annu Rev Physiol,2006,68:307-343.
13 .Hennig GW,Spencer NJ,Jokela-Willis,et al.ICC-MY coordinate smooth muscle electrical and mechanical activity in the murine small intestine[J].Neurogastroenterol Motil,2010,22(5):e138-151.
14 .Klein S,Seidler B,Kettenberger A,et al.Interstitial cells of Cajal integrate excitatory and inhibitory neurotransmission with intestinal slow-wave activity[J].Nat Commun,2013,4:1630.
15 .Chen J,Du L,Xiao YT,et al.Disruption of interstitial cells of Cajal networks after massive small bowel resection[J].World J Gastroenterol,2013,19(22):3415-6422.
16 .Villanacci V,Annese V,Cuttitta A,et al.An immunohistochemical study of the myenteric plexus in idiopathic achalasia[J].J Clin Gastroenterol,2010,44(6):407-410.
17 .Bassotti G,Villanacci V,Maurer CA,et al.The role of glial cells and apoptosis of enteric neurones in the neuropathology of intractable slow transit constipation[J].Gut,2006,55(1):41-46.
18 .Rolle U,Piaseczna-Piotrowska A,Puri P.Interstitial cells of Cajal in the normal gut and in intestinal motility disorders of childhood[J].Pediatr Surg Int,2007,23(12):1139-1152.
19 .Choi KM,Gibbons SJ,Roeder JL,et al.Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide[J].Neurogastroenterol Motil,2007,19(7):585-595.
2 .Debreceni A,Abdel-Salam OM,Figler M,et al.Capsaicin increases gastric emptying rate in healthy human subjects measured by 13C-labeled octanoic acid breath test[J].J Physiol Paris,1999,93(5):455-460.
3 .Matsumoto K,Kurosawa E,Terui H,et al.Localization of TRPV1 and contractile effect of capsaicin in mouse large intestine:high abundance and sensitivity in rectum and distal colon[J].Am J Physiol Gastrointest Liver Physiol,2009,297(2):G348-360.
4 .Bartho L,Lenard L,Patacchini R,et al.Tachykinin receptors are involved in the"local efferent"motor response to capsaicin in the guinea-pig small intestine and oesophagus[J].Neuroscience,1999,90(1):221-228.
5 .Bartho L,Benko R,Patacchini R,et al.Effects of capsaicin on visceral smooth muscle:a valuable tool for sensory neurotransmitter identification[J].Eur J Pharmacol,2004,500(1-3):143-157.
6 .李毅,齐清会.胃肠道神经-Cajal间质细胞-平滑肌网络研究进展[J].国际消化病杂志2007,27(3):225-227.
7 .Lammers WJ,Stephen B.Origin and propagation of individual slow waves along the intact feline small intestine[J].Exp Physiol,2008,93(3):334-346.
8 .Apoznanski W,Koleda P,Wozniak Z,et al.The distribution of interstitial cells of Cajal in congenital ureteropelvic junction obstruction[J].Int Urol Nephrol,2013,45(3):6077-612.
9 .张虹玺.养荣润肠舒合剂对慢传输型便秘模型大鼠结肠中c-Kit、SP与VIP的影响[J].中国药房,2015,26(25):3476-3478.
10 .袁成代,高庆剑.温阳健脾法治疗便秘50例[J].河南中医,2015,35(7):1619-1620.
11 .Huizinga JD,Zarate N,Farrugia G.Physiology,injury,and recovery of interstitial cells of Cajal:basic and clinical science[J].Gastroenterology,2009,137(5):1548-1556.
12 .Sanders KM,Koh SD,Ward SM.Interstitial cells of cajal as pacemakers in the gastrointestinal tract[J].Annu Rev Physiol,2006,68:307-343.
13 .Hennig GW,Spencer NJ,Jokela-Willis,et al.ICC-MY coordinate smooth muscle electrical and mechanical activity in the murine small intestine[J].Neurogastroenterol Motil,2010,22(5):e138-151.
14 .Klein S,Seidler B,Kettenberger A,et al.Interstitial cells of Cajal integrate excitatory and inhibitory neurotransmission with intestinal slow-wave activity[J].Nat Commun,2013,4:1630.
15 .Chen J,Du L,Xiao YT,et al.Disruption of interstitial cells of Cajal networks after massive small bowel resection[J].World J Gastroenterol,2013,19(22):3415-6422.
16 .Villanacci V,Annese V,Cuttitta A,et al.An immunohistochemical study of the myenteric plexus in idiopathic achalasia[J].J Clin Gastroenterol,2010,44(6):407-410.
17 .Bassotti G,Villanacci V,Maurer CA,et al.The role of glial cells and apoptosis of enteric neurones in the neuropathology of intractable slow transit constipation[J].Gut,2006,55(1):41-46.
18 .Rolle U,Piaseczna-Piotrowska A,Puri P.Interstitial cells of Cajal in the normal gut and in intestinal motility disorders of childhood[J].Pediatr Surg Int,2007,23(12):1139-1152.
19 .Choi KM,Gibbons SJ,Roeder JL,et al.Regulation of interstitial cells of Cajal in the mouse gastric body by neuronal nitric oxide[J].Neurogastroenterol Motil,2007,19(7):585-595.