火炬树根水浸提液对黑心菊土壤微生物碳源利用的影响
|
摘要
为研究火炬树根对黑心菊根际土壤微生物的影响,采用Biolog-ECO微平板培养技术,探讨黑心菊土壤微生物碳源利用,及对不同质量浓度火炬树根水浸提液的响应。结果表明:C处理(火炬树根水浸提液质量浓度为0.3 g·mL~(-1))的土壤有机碳质量分数、总氮质量分数、土壤p H及电导率最高,但土壤含水率无显著差异;在不同质量浓度火炬树根水浸提液处理下,单孔颜色平均值均随着培养时间的增加而逐渐上升,在处理156 h时均达到最大值;C处理碳源利用最大,D处理(火炬树根水浸提液质量浓度为0.4 g·mL~(-1))碳源利用最小。不同质量浓度的火炬树根水浸提液均促进了黑心菊土壤微生物对碳水化合物、羧酸的利用,抑制了对多聚物、氨基酸和胺类物质的利用,其中C处理对酚酸类利用率最大。土壤微生物碳源利用的Shannon-Wiener多样性指数、Pielou均匀度指数及Gini多样性指数均存在显著差异(P<0.05)。冗余分析表明有机碳是影响土壤微生物碳源利用的主要因素,C处理碳源利用最强,A处理(火炬树根水浸提液质量浓度为0.1 g·mL~(-1))碳源利用最差。
In order to study the influence of Rhus typhina roots on the rhizosphere soil microorganism of Rudbeckia hirta,the Biolog-ECO micro plate culture technology was adopted to explore the use of microbial carbon source utilization in the soil of Rudbeckia hirta,and to response to different aqueous extract contents of the roots of R. typhina. The soil organic carbon content,total nitrogen content,soil pH and electrical conductivity were the highest in C treatment( the content of R. typhina aqueous extract was 0.3 g·mL~(-1)). However,there was no significant difference in soil moisture content. Under different contents of aqueous extracts,the average well color development gradually increased with the culture time,and reached the maximum value at 156 h. The carbon source utilization of the soil microorganisms was the highest in C treatment,D treatment( the content of R. typhina aqueous extract was 0.4 g·mL~(-1)) was the lowest. Different contents of aqueous extract of the roots of R. typhina promoted the utilization of carbohydrate and carboxylic acid of soil microorganism of R. hirta.But the utilization of polymers,amino acids and amines were inhibited,and the most use of phenolic acids was C treatment. There were significant differences in the diversity index of Shannon-Wiener,Pielou index and Gini index of soil microbial carbon sources utilization( P<0.05). By redundancy analysis,organic carbon was the main factor influencing the utilization of soil microbial carbon source. According to the main content sequence,carbon source utilized by C treatment was the strongest,and carbon source utilized by A treatment(the concentration of Rhus typhina aqueous extract was 0.1 g·mL~(-1)) was the worst.
In order to study the influence of Rhus typhina roots on the rhizosphere soil microorganism of Rudbeckia hirta,the Biolog-ECO micro plate culture technology was adopted to explore the use of microbial carbon source utilization in the soil of Rudbeckia hirta,and to response to different aqueous extract contents of the roots of R. typhina. The soil organic carbon content,total nitrogen content,soil pH and electrical conductivity were the highest in C treatment( the content of R. typhina aqueous extract was 0.3 g·mL~(-1)). However,there was no significant difference in soil moisture content. Under different contents of aqueous extracts,the average well color development gradually increased with the culture time,and reached the maximum value at 156 h. The carbon source utilization of the soil microorganisms was the highest in C treatment,D treatment( the content of R. typhina aqueous extract was 0.4 g·mL~(-1)) was the lowest. Different contents of aqueous extract of the roots of R. typhina promoted the utilization of carbohydrate and carboxylic acid of soil microorganism of R. hirta.But the utilization of polymers,amino acids and amines were inhibited,and the most use of phenolic acids was C treatment. There were significant differences in the diversity index of Shannon-Wiener,Pielou index and Gini index of soil microbial carbon sources utilization( P<0.05). By redundancy analysis,organic carbon was the main factor influencing the utilization of soil microbial carbon source. According to the main content sequence,carbon source utilized by C treatment was the strongest,and carbon source utilized by A treatment(the concentration of Rhus typhina aqueous extract was 0.1 g·mL~(-1)) was the worst.
引文
[1]陈颖,李晨光,徐学华,等.铁尾矿废弃地火炬树林水土保持功能[J].水土保持学报,2015,29(3):106-111.
[2]章岳涛,谭飞理,李广德,等.片麻岩石质山地火炬树幼林集水保墒措施优化[J].东北林业大学学报,2013,41(5):51-55.
[3]赵艳军.干旱地区火炬树耐旱性测定[J].青海农林科技,2006(2):19-20.
[4]段艳芳,赵辉,樊巍.太行山南端外来树种火炬树群落的水保效应[J].中国农学通报,2012,28(25):43-46.
[5]陈印平,刘俊华.盐胁迫下火炬树水浸液对小麦种子萌发的化感作用[J].滨州学院学报,2017,33(4):59-64.
[6]贾波,曹帮华,庞丙亮,等.不同盐碱胁迫对火炬树种子萌发的影响[J].山东科学,2009,22(4):19-23.
[7]陆秀君,刁诚轩,邹永田,等.壳聚糖辅助下火炬树修复铅污染土壤潜力及其生长[J].北方园艺,2010(14):34-38.
[8]林瑛,王策,信思宇.火炬树幼苗镉含量及分布特征的研究[J].辽宁林业科技,2011(4):19-21.
[9]王大成.火炬树果穗的次生代谢产物及其生物活性研究[D].长春:吉林大学,2014.
[10]张恒庆,穆晓红,贾鑫,等.大连地区外来物种火炬树的调查研究[J].吉林师范大学学报(自然科学版),2018,39(2):104-107.
[11]黄乔乔,许慧,范志伟,等.火炬树入侵黑松幼林过程中对土壤化学性质的影响[J].生态环境学报,2013,22(7):1119-1123.
[12]卜庆梅,侯玉平,房洪坤,等.外来树种火炬树入侵的生理生态特性:与同属本地种盐肤木的比较[J].林业科学,2017,53(5):1-7.
[13]樊巍,高喜荣,赵东,等.太行山退化山地火炬树群落物种多样性与土壤特性变化的研究[J].河南农业大学学报,2008,42(3):299-302.
[14]曲同宝,李怡莹,马文育.火炬树水浸提液对波斯菊种子萌发和幼苗生长的影响[J].东北林业大学学报,2017,45(11):26-31.
[15]许桂芳,许明录,刘明久.火炬树叶片腐解物浸提液对上海青种苗生长的影响[J].生态环境学报,2010,19(10):2313-2317.
[16]闫兴富,杜茜,方苏,等.火炬树水浸提液对玉米种子萌发的化感效应[J].种子,2010,29(3):15-18.
[17]白丽荣,时丽冉,徐振华,等.火炬树浸提液对几种农作物的化感作用[J].种子,2010,29(6):91-93.
[18]闫兴福,周云峰,杜茜.火炬树根和凋落叶水浸提液对小麦种子萌发的化感作用[J].种子,2011,30(5):17-20.
[19]闫兴富,方苏,杜茜,等.火炬树果穗水浸提液对小麦种子萌发的化感效应[J].作物杂志,2009(6):38-41.
[20] MA J,BU R C,LIU M,et al. Ecosystem carbon storage distribution between plant and soil in different forest types in northeastern china[J]. Ecological Engineering,2015,81:353-362.
[21] YANG D W,ZHANG M K. Effects of land-use conversion from paddy field to orchard farm on soil microbial genetic diversity and community structure[J]. European Journal of Soil Biology,2014,64:30-39.
[22]侯玉平,柳林,王信,等.外来植物火炬树水浸液对土壤微生态系统的化感作用[J].生态学报,2013,33(13):4041-4049.
[23]廖亚丽.入侵植物加拿大一枝黄花凋落叶的化感作用及机理研究[D].镇江:江苏大学,2017.
[24]杨琼,梁羽,杨剑,等.入侵植物薇甘菊的根际土壤微生物特征[J].生态科学,2015,34(2):148-155.
[25]林兴生,林占熺,林冬梅,等.荒坡地种植巨菌草对土壤微生物群落功能多样性及土壤肥力的影响[J].生态学报,2014,34(15):4304-4312.
[26] ROGERS B F,TATE R L. Temporal analysis of the soil microbial community along a top sequence in Pineland soils[J]. Soil Biology and Biochemistry,2001,33(10):1389-1401.
[27]鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社,2008.
[28]安韶山,李国辉,陈利顶.宁南山区典型植物根际与非根际土壤微生物功能多样性[J].生态学报,2011,31(18):5225-5234.
[29]张明莉,常宏磊,马淼.基于Biolog技术的外来种意大利苍耳与本地种苍耳根际土壤微生物功能多样性的比较[J].草业学报,2017,26(10):179-187.
[30] LI W H,ZHANG C B,JIANG H B,et al. Changes in soil microbial community associated with invasion of the exotic weed,Mikania micrantha HBK[J]. Plant and Soil,2006,281(1/2):309-324.
[31] LIAO C Z,LUO Y Q,JIANG L F,et al. Invasion of Spartina alterniflora enhanced ecosystem carbon and nitrogen stocks in the Yangtze Estuary,China[J]. Ecosystems,2007,10(8):1351-1361.
[32] ALLISON S D,VITOUSEK P M. Rapid nutrient cycling in leaf litter from invasive plants in Hawai’I[J]. Oecologia,2004,141(4):612-619.
[33] DUDA J J,FREEMAN D C,EMLEN J M,et al. Differences in native soil ecology associated with invasion of the exotic annual chenopod,Halogeton glomeratus[J]. Biology and Fertility of soils,2003,38(2):72-77.
[34]陈学林,张丽娜,王桔红,等.红毛草入侵程度对根际土壤微生物的影响[J].西北农林科技大学学报(自然科学版),2017,45(4):1-8.
[35]牛红榜,刘万学,万方浩.紫茎泽兰(Ageratinaad adenophora)对土壤微生物群落和理化性质的影响[J].生态学报,2007,27(7):3051-3060.
[36]侯玉平,柳林,初航,等.外来植物火炬树(Rhus typhina L.)入侵对不同林型土壤性质的影响[J].生态学报,2015,35(16):5325-5331.
[37]胡可,王利宾.BIOLOG微平板技术在土壤微生态研究中的应用[J].土壤通报,2007,38(4):819-821.
[38]李鑫,张会慧,岳冰冰,等.高羊茅草对盐碱地原油污染土壤微生物的影响[J].应用生态学报,2012,23(12):3414-3420.
[39]马玲,马琨,汤梦洁,等.间作与接种AMF对连作土壤微生物群落结构与功能的影响[J].生态环境学报,2013,22(8):1341-1347.
[40]张静,温仲明,李鸣雷,等.外来物种刺槐对土壤微生物功能多样性的影响[J].生态学报,2018,38(14):1-10.
[41]刘秉儒,张秀珍,胡天华,等.贺兰山不同海拔典型植被带土壤微生物多样性[J].生态学报,2013,33(22):7211-7220.
[42] CARACCIOLO A B,BUSTAMANTE M A,NOGUES I,et al.Changes in microbial community structure and functioning of a semiarid soil due to the use of anaerobic digestate derived composts and rosemary plants[J]. Geoderma,2015,245/246:89-97.
[2]章岳涛,谭飞理,李广德,等.片麻岩石质山地火炬树幼林集水保墒措施优化[J].东北林业大学学报,2013,41(5):51-55.
[3]赵艳军.干旱地区火炬树耐旱性测定[J].青海农林科技,2006(2):19-20.
[4]段艳芳,赵辉,樊巍.太行山南端外来树种火炬树群落的水保效应[J].中国农学通报,2012,28(25):43-46.
[5]陈印平,刘俊华.盐胁迫下火炬树水浸液对小麦种子萌发的化感作用[J].滨州学院学报,2017,33(4):59-64.
[6]贾波,曹帮华,庞丙亮,等.不同盐碱胁迫对火炬树种子萌发的影响[J].山东科学,2009,22(4):19-23.
[7]陆秀君,刁诚轩,邹永田,等.壳聚糖辅助下火炬树修复铅污染土壤潜力及其生长[J].北方园艺,2010(14):34-38.
[8]林瑛,王策,信思宇.火炬树幼苗镉含量及分布特征的研究[J].辽宁林业科技,2011(4):19-21.
[9]王大成.火炬树果穗的次生代谢产物及其生物活性研究[D].长春:吉林大学,2014.
[10]张恒庆,穆晓红,贾鑫,等.大连地区外来物种火炬树的调查研究[J].吉林师范大学学报(自然科学版),2018,39(2):104-107.
[11]黄乔乔,许慧,范志伟,等.火炬树入侵黑松幼林过程中对土壤化学性质的影响[J].生态环境学报,2013,22(7):1119-1123.
[12]卜庆梅,侯玉平,房洪坤,等.外来树种火炬树入侵的生理生态特性:与同属本地种盐肤木的比较[J].林业科学,2017,53(5):1-7.
[13]樊巍,高喜荣,赵东,等.太行山退化山地火炬树群落物种多样性与土壤特性变化的研究[J].河南农业大学学报,2008,42(3):299-302.
[14]曲同宝,李怡莹,马文育.火炬树水浸提液对波斯菊种子萌发和幼苗生长的影响[J].东北林业大学学报,2017,45(11):26-31.
[15]许桂芳,许明录,刘明久.火炬树叶片腐解物浸提液对上海青种苗生长的影响[J].生态环境学报,2010,19(10):2313-2317.
[16]闫兴富,杜茜,方苏,等.火炬树水浸提液对玉米种子萌发的化感效应[J].种子,2010,29(3):15-18.
[17]白丽荣,时丽冉,徐振华,等.火炬树浸提液对几种农作物的化感作用[J].种子,2010,29(6):91-93.
[18]闫兴福,周云峰,杜茜.火炬树根和凋落叶水浸提液对小麦种子萌发的化感作用[J].种子,2011,30(5):17-20.
[19]闫兴富,方苏,杜茜,等.火炬树果穗水浸提液对小麦种子萌发的化感效应[J].作物杂志,2009(6):38-41.
[20] MA J,BU R C,LIU M,et al. Ecosystem carbon storage distribution between plant and soil in different forest types in northeastern china[J]. Ecological Engineering,2015,81:353-362.
[21] YANG D W,ZHANG M K. Effects of land-use conversion from paddy field to orchard farm on soil microbial genetic diversity and community structure[J]. European Journal of Soil Biology,2014,64:30-39.
[22]侯玉平,柳林,王信,等.外来植物火炬树水浸液对土壤微生态系统的化感作用[J].生态学报,2013,33(13):4041-4049.
[23]廖亚丽.入侵植物加拿大一枝黄花凋落叶的化感作用及机理研究[D].镇江:江苏大学,2017.
[24]杨琼,梁羽,杨剑,等.入侵植物薇甘菊的根际土壤微生物特征[J].生态科学,2015,34(2):148-155.
[25]林兴生,林占熺,林冬梅,等.荒坡地种植巨菌草对土壤微生物群落功能多样性及土壤肥力的影响[J].生态学报,2014,34(15):4304-4312.
[26] ROGERS B F,TATE R L. Temporal analysis of the soil microbial community along a top sequence in Pineland soils[J]. Soil Biology and Biochemistry,2001,33(10):1389-1401.
[27]鲍士旦.土壤农化分析[M]. 3版.北京:中国农业出版社,2008.
[28]安韶山,李国辉,陈利顶.宁南山区典型植物根际与非根际土壤微生物功能多样性[J].生态学报,2011,31(18):5225-5234.
[29]张明莉,常宏磊,马淼.基于Biolog技术的外来种意大利苍耳与本地种苍耳根际土壤微生物功能多样性的比较[J].草业学报,2017,26(10):179-187.
[30] LI W H,ZHANG C B,JIANG H B,et al. Changes in soil microbial community associated with invasion of the exotic weed,Mikania micrantha HBK[J]. Plant and Soil,2006,281(1/2):309-324.
[31] LIAO C Z,LUO Y Q,JIANG L F,et al. Invasion of Spartina alterniflora enhanced ecosystem carbon and nitrogen stocks in the Yangtze Estuary,China[J]. Ecosystems,2007,10(8):1351-1361.
[32] ALLISON S D,VITOUSEK P M. Rapid nutrient cycling in leaf litter from invasive plants in Hawai’I[J]. Oecologia,2004,141(4):612-619.
[33] DUDA J J,FREEMAN D C,EMLEN J M,et al. Differences in native soil ecology associated with invasion of the exotic annual chenopod,Halogeton glomeratus[J]. Biology and Fertility of soils,2003,38(2):72-77.
[34]陈学林,张丽娜,王桔红,等.红毛草入侵程度对根际土壤微生物的影响[J].西北农林科技大学学报(自然科学版),2017,45(4):1-8.
[35]牛红榜,刘万学,万方浩.紫茎泽兰(Ageratinaad adenophora)对土壤微生物群落和理化性质的影响[J].生态学报,2007,27(7):3051-3060.
[36]侯玉平,柳林,初航,等.外来植物火炬树(Rhus typhina L.)入侵对不同林型土壤性质的影响[J].生态学报,2015,35(16):5325-5331.
[37]胡可,王利宾.BIOLOG微平板技术在土壤微生态研究中的应用[J].土壤通报,2007,38(4):819-821.
[38]李鑫,张会慧,岳冰冰,等.高羊茅草对盐碱地原油污染土壤微生物的影响[J].应用生态学报,2012,23(12):3414-3420.
[39]马玲,马琨,汤梦洁,等.间作与接种AMF对连作土壤微生物群落结构与功能的影响[J].生态环境学报,2013,22(8):1341-1347.
[40]张静,温仲明,李鸣雷,等.外来物种刺槐对土壤微生物功能多样性的影响[J].生态学报,2018,38(14):1-10.
[41]刘秉儒,张秀珍,胡天华,等.贺兰山不同海拔典型植被带土壤微生物多样性[J].生态学报,2013,33(22):7211-7220.
[42] CARACCIOLO A B,BUSTAMANTE M A,NOGUES I,et al.Changes in microbial community structure and functioning of a semiarid soil due to the use of anaerobic digestate derived composts and rosemary plants[J]. Geoderma,2015,245/246:89-97.